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O94201

- PFKA1_CANAX

UniProt

O94201 - PFKA1_CANAX

Protein

ATP-dependent 6-phosphofructokinase subunit alpha

Gene

PFK1

Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei237 – 2371ATP; via amide nitrogenUniRule annotation
    Metal bindingi331 – 3311Magnesium; catalyticUniRule annotation
    Active sitei378 – 3781Proton acceptorUniRule annotation
    Binding sitei413 – 4131Substrate; shared with subunit betaUniRule annotation
    Binding sitei477 – 4771SubstrateUniRule annotation
    Binding sitei504 – 5041Substrate; shared with subunit betaUniRule annotation
    Binding sitei686 – 6861Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei781 – 7811Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
    Binding sitei848 – 8481Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei874 – 8741Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
    Binding sitei958 – 9581Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi300 – 3012ATPUniRule annotation
    Nucleotide bindingi330 – 3334ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation (EC:2.7.1.11UniRule annotation)
    Alternative name(s):
    ATP-dependent 6-phosphofructokinase 1UniRule annotation
    Short name:
    ATP-PFK 1UniRule annotation
    Short name:
    Phosphofructokinase 1UniRule annotation
    Phosphohexokinase 1UniRule annotation
    Gene namesi
    Name:PFK1
    OrganismiCandida albicans (Yeast)
    Taxonomic identifieri5476 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 987987ATP-dependent 6-phosphofructokinase subunit alphaPRO_0000112038Add
    BLAST

    Interactioni

    Subunit structurei

    Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliO94201.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 602602N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni376 – 3783Substrate bindingUniRule annotation
    Regioni420 – 4223Substrate bindingUniRule annotation
    Regioni510 – 5134Substrate bindingUniRule annotation
    Regioni603 – 61614Interdomain linkerAdd
    BLAST
    Regioni617 – 987371C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni743 – 7475Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni788 – 7903Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni880 – 8834Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94201-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSSSDAINR ISYISLVTSD NDKFNQTFQF YSQLGFRLTK SFSKVSSYGS    50
    GLGANHPEFQ LGVSHDSLKE VWLESYPLQN VDSNGNLRPW QEMEVYDGDN 100
    CERLNESTVI KVRLLGETPL KSISQKQFVF FTTQLNKIEK ILTDANVKYG 150
    KVVDNVILAE DPLNNIISFS NTQNELCKTR FQSPEEYVEK TTAEILAKRK 200
    KSQLGSKFGS FEEISPSEVG GGNGLRKKKI GVMTSGGDAP GMNPAVRAVV 250
    RAGIYYGCDV YAVYEGYEGL VKGGDLLKKM EWSDVRSYMS LGGTSIGTAR 300
    CKEFRERAGR LQGAYNMIKN GIDALVVCGG DGSLTGADLF RSEWPSLVKE 350
    LVDTGKLTKE EVSPYEHLTI VGLVGSIDND MSGTDVTIGA FSALERITEM 400
    VDYIGATAAS HSRAFVVEVM GRHCGWLALL SGLATGADFV FIPERPPKAG 450
    LWKEQLKEVC LRHREYGRRK TTVIVAEGAI DDELNPITSE EVKQVLADLG 500
    LDTRNTILGH VQRGGTAVAF DRRLATLQGV EAVKAVLEMT PDTPSPMIGI 550
    LKHKIVRIPL VDAVKQTKAV AEAISNKDFD KAMSLRDNSF YDDYRYFRDI 600
    SIYDDGSKQL SEDKRLNIAI VHVGAASAGL NAATRAVALY SLSRGHKLYA 650
    VQDGFAGLVK GDLKNLTWMD VEGWHSLGGS EIGTNRSLPS QNIGKVAYNL 700
    QKFNIQGLLI VGGFEAFTSL HELSEQKANY PIFEIPMVVV PATVSNNVPG 750
    TEYSLGADTC LNQLVSYCDA VQQSASSTRR RVFVVEVQGG HSGYVASYCG 800
    LITGALATYT PESNINLREL QGDIDLLQKV FATDRGEDHN GTLIVRNEQA 850
    SAVYSTQLIA DILKENANKR FETRTAIPGH VQQGFTPSAN DRVMAVKFSL 900
    KAMEFIETRN GCYGKHDRKF SDEEISEHSQ VVIGIHGDVV KFTCIKHLYD 950
    NEANVALRKG KTVHWTDMID VANILNGKSL LKKQERY 987
    Length:987
    Mass (Da):108,598
    Last modified:May 1, 1999 - v1
    Checksum:i4EE5BAB6D02349FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ007638 Genomic DNA. Translation: CAB38868.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ007638 Genomic DNA. Translation: CAB38868.1 .

    3D structure databases

    ProteinModelPortali O94201.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0205.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans."
      Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J.
      Eur. J. Biochem. 260:217-226(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594.

    Entry informationi

    Entry nameiPFKA1_CANAX
    AccessioniPrimary (citable) accession number: O94201
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3