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O94201

- PFKA1_CANAX

UniProt

O94201 - PFKA1_CANAX

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Protein
ATP-dependent 6-phosphofructokinase subunit alpha
Gene
PFK1
Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei237 – 2371ATP; via amide nitrogen By similarity
Metal bindingi331 – 3311Magnesium; catalytic By similarity
Active sitei378 – 3781Proton acceptor By similarity
Binding sitei413 – 4131Substrate; shared with subunit beta By similarity
Binding sitei477 – 4771Substrate By similarity
Binding sitei504 – 5041Substrate; shared with subunit beta By similarity
Binding sitei686 – 6861Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei781 – 7811Allosteric activator fructose 2,6-bisphosphate; shared with subunit beta By similarity
Binding sitei848 – 8481Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei874 – 8741Allosteric activator fructose 2,6-bisphosphate; shared with subunit beta By similarity
Binding sitei958 – 9581Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi300 – 3012ATP By similarity
Nucleotide bindingi330 – 3334ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit alpha (EC:2.7.1.11)
Alternative name(s):
ATP-dependent 6-phosphofructokinase 1
Short name:
ATP-PFK 1
Short name:
Phosphofructokinase 1
Phosphohexokinase 1
Gene namesi
Name:PFK1
OrganismiCandida albicans (Yeast)
Taxonomic identifieri5476 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 987987ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation
PRO_0000112038Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains By similarity.

Structurei

3D structure databases

ProteinModelPortaliO94201.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 602602N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni376 – 3783Substrate binding By similarity
Regioni420 – 4223Substrate binding By similarity
Regioni510 – 5134Substrate binding By similarity
Regioni603 – 61614Interdomain linkerUniRule annotation
Add
BLAST
Regioni617 – 987371C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni743 – 7475Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni788 – 7903Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni880 – 8834Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94201-1 [UniParc]FASTAAdd to Basket

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MPSSSDAINR ISYISLVTSD NDKFNQTFQF YSQLGFRLTK SFSKVSSYGS    50
GLGANHPEFQ LGVSHDSLKE VWLESYPLQN VDSNGNLRPW QEMEVYDGDN 100
CERLNESTVI KVRLLGETPL KSISQKQFVF FTTQLNKIEK ILTDANVKYG 150
KVVDNVILAE DPLNNIISFS NTQNELCKTR FQSPEEYVEK TTAEILAKRK 200
KSQLGSKFGS FEEISPSEVG GGNGLRKKKI GVMTSGGDAP GMNPAVRAVV 250
RAGIYYGCDV YAVYEGYEGL VKGGDLLKKM EWSDVRSYMS LGGTSIGTAR 300
CKEFRERAGR LQGAYNMIKN GIDALVVCGG DGSLTGADLF RSEWPSLVKE 350
LVDTGKLTKE EVSPYEHLTI VGLVGSIDND MSGTDVTIGA FSALERITEM 400
VDYIGATAAS HSRAFVVEVM GRHCGWLALL SGLATGADFV FIPERPPKAG 450
LWKEQLKEVC LRHREYGRRK TTVIVAEGAI DDELNPITSE EVKQVLADLG 500
LDTRNTILGH VQRGGTAVAF DRRLATLQGV EAVKAVLEMT PDTPSPMIGI 550
LKHKIVRIPL VDAVKQTKAV AEAISNKDFD KAMSLRDNSF YDDYRYFRDI 600
SIYDDGSKQL SEDKRLNIAI VHVGAASAGL NAATRAVALY SLSRGHKLYA 650
VQDGFAGLVK GDLKNLTWMD VEGWHSLGGS EIGTNRSLPS QNIGKVAYNL 700
QKFNIQGLLI VGGFEAFTSL HELSEQKANY PIFEIPMVVV PATVSNNVPG 750
TEYSLGADTC LNQLVSYCDA VQQSASSTRR RVFVVEVQGG HSGYVASYCG 800
LITGALATYT PESNINLREL QGDIDLLQKV FATDRGEDHN GTLIVRNEQA 850
SAVYSTQLIA DILKENANKR FETRTAIPGH VQQGFTPSAN DRVMAVKFSL 900
KAMEFIETRN GCYGKHDRKF SDEEISEHSQ VVIGIHGDVV KFTCIKHLYD 950
NEANVALRKG KTVHWTDMID VANILNGKSL LKKQERY 987
Length:987
Mass (Da):108,598
Last modified:May 1, 1999 - v1
Checksum:i4EE5BAB6D02349FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007638 Genomic DNA. Translation: CAB38868.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007638 Genomic DNA. Translation: CAB38868.1 .

3D structure databases

ProteinModelPortali O94201.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0205.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans."
    Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J.
    Eur. J. Biochem. 260:217-226(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594.

Entry informationi

Entry nameiPFKA1_CANAX
AccessioniPrimary (citable) accession number: O94201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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