Reviewed, UniProtKB/Swiss-Prot O94201 (K6PF1_CANAL)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase subunit alpha
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 1
    Phosphohexokinase
    6PF-1-K subunit alpha
    CaPFK1

Gene names

Name:

PFK1

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	987 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 987	987	6-phosphofructokinase subunit alpha		PRO_0000112038
Regions
	Nucleotide binding	247 – 251	5	ATP By similarity
	Nucleotide binding	405 – 409	5	ATP By similarity
	Nucleotide binding	422 – 438	17	ATP By similarity
Sites
	Active site	378	1	Proton acceptor By similarity
	Metal binding	436	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	413	1	Substrate By similarity
	Binding site	504	1	Substrate By similarity
	Binding site	510	1	Substrate By similarity
	Binding site	513	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94201-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 4EE5BAB6D02349FA	FASTA	987	108,598
	10 20 30 40 50 60 MPSSSDAINR ISYISLVTSD NDKFNQTFQF YSQLGFRLTK SFSKVSSYGS GLGANHPEFQ 70 80 90 100 110 120 LGVSHDSLKE VWLESYPLQN VDSNGNLRPW QEMEVYDGDN CERLNESTVI KVRLLGETPL 130 140 150 160 170 180 KSISQKQFVF FTTQLNKIEK ILTDANVKYG KVVDNVILAE DPLNNIISFS NTQNELCKTR 190 200 210 220 230 240 FQSPEEYVEK TTAEILAKRK KSQLGSKFGS FEEISPSEVG GGNGLRKKKI GVMTSGGDAP 250 260 270 280 290 300 GMNPAVRAVV RAGIYYGCDV YAVYEGYEGL VKGGDLLKKM EWSDVRSYMS LGGTSIGTAR 310 320 330 340 350 360 CKEFRERAGR LQGAYNMIKN GIDALVVCGG DGSLTGADLF RSEWPSLVKE LVDTGKLTKE 370 380 390 400 410 420 EVSPYEHLTI VGLVGSIDND MSGTDVTIGA FSALERITEM VDYIGATAAS HSRAFVVEVM 430 440 450 460 470 480 GRHCGWLALL SGLATGADFV FIPERPPKAG LWKEQLKEVC LRHREYGRRK TTVIVAEGAI 490 500 510 520 530 540 DDELNPITSE EVKQVLADLG LDTRNTILGH VQRGGTAVAF DRRLATLQGV EAVKAVLEMT 550 560 570 580 590 600 PDTPSPMIGI LKHKIVRIPL VDAVKQTKAV AEAISNKDFD KAMSLRDNSF YDDYRYFRDI 610 620 630 640 650 660 SIYDDGSKQL SEDKRLNIAI VHVGAASAGL NAATRAVALY SLSRGHKLYA VQDGFAGLVK 670 680 690 700 710 720 GDLKNLTWMD VEGWHSLGGS EIGTNRSLPS QNIGKVAYNL QKFNIQGLLI VGGFEAFTSL 730 740 750 760 770 780 HELSEQKANY PIFEIPMVVV PATVSNNVPG TEYSLGADTC LNQLVSYCDA VQQSASSTRR 790 800 810 820 830 840 RVFVVEVQGG HSGYVASYCG LITGALATYT PESNINLREL QGDIDLLQKV FATDRGEDHN 850 860 870 880 890 900 GTLIVRNEQA SAVYSTQLIA DILKENANKR FETRTAIPGH VQQGFTPSAN DRVMAVKFSL 910 920 930 940 950 960 KAMEFIETRN GCYGKHDRKF SDEEISEHSQ VVIGIHGDVV KFTCIKHLYD NEANVALRKG 970 980 KTVHWTDMID VANILNGKSL LKKQERY

References

[1]

"Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans." Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J. Eur. J. Biochem. 260:217-226(1999) [PubMed: 10091602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 10231 / CBS 6431 / DSM 1386 / IFO 1594.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ007638 Genomic DNA. Translation: CAB38868.1.
3D structure databases
HSSP	HSSP built from PDB template 1MTO based on UniProtKB P00512.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.11. 1124.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_CANAL

Accession

Primary (citable) accession number: O94201

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)