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O94201

- PFKA1_CANAX

UniProt

O94201 - PFKA1_CANAX

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Protein

ATP-dependent 6-phosphofructokinase subunit alpha

Gene

PFK1

Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei237 – 2371ATP; via amide nitrogenUniRule annotation
Metal bindingi331 – 3311Magnesium; catalyticUniRule annotation
Active sitei378 – 3781Proton acceptorUniRule annotation
Binding sitei413 – 4131Substrate; shared with subunit betaUniRule annotation
Binding sitei477 – 4771SubstrateUniRule annotation
Binding sitei504 – 5041Substrate; shared with subunit betaUniRule annotation
Binding sitei686 – 6861Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei781 – 7811Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
Binding sitei848 – 8481Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei874 – 8741Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
Binding sitei958 – 9581Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi300 – 3012ATPUniRule annotation
Nucleotide bindingi330 – 3334ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation (EC:2.7.1.11UniRule annotation)
Alternative name(s):
ATP-dependent 6-phosphofructokinase 1UniRule annotation
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:PFK1
OrganismiCandida albicans (Yeast)
Taxonomic identifieri5476 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 987987ATP-dependent 6-phosphofructokinase subunit alphaPRO_0000112038Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO94201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 602602N-terminal catalytic PFK domain 1Add
BLAST
Regioni376 – 3783Substrate bindingUniRule annotation
Regioni420 – 4223Substrate bindingUniRule annotation
Regioni510 – 5134Substrate bindingUniRule annotation
Regioni603 – 61614Interdomain linkerAdd
BLAST
Regioni617 – 987371C-terminal regulatory PFK domain 2Add
BLAST
Regioni743 – 7475Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni788 – 7903Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni880 – 8834Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94201-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSSSDAINR ISYISLVTSD NDKFNQTFQF YSQLGFRLTK SFSKVSSYGS
60 70 80 90 100
GLGANHPEFQ LGVSHDSLKE VWLESYPLQN VDSNGNLRPW QEMEVYDGDN
110 120 130 140 150
CERLNESTVI KVRLLGETPL KSISQKQFVF FTTQLNKIEK ILTDANVKYG
160 170 180 190 200
KVVDNVILAE DPLNNIISFS NTQNELCKTR FQSPEEYVEK TTAEILAKRK
210 220 230 240 250
KSQLGSKFGS FEEISPSEVG GGNGLRKKKI GVMTSGGDAP GMNPAVRAVV
260 270 280 290 300
RAGIYYGCDV YAVYEGYEGL VKGGDLLKKM EWSDVRSYMS LGGTSIGTAR
310 320 330 340 350
CKEFRERAGR LQGAYNMIKN GIDALVVCGG DGSLTGADLF RSEWPSLVKE
360 370 380 390 400
LVDTGKLTKE EVSPYEHLTI VGLVGSIDND MSGTDVTIGA FSALERITEM
410 420 430 440 450
VDYIGATAAS HSRAFVVEVM GRHCGWLALL SGLATGADFV FIPERPPKAG
460 470 480 490 500
LWKEQLKEVC LRHREYGRRK TTVIVAEGAI DDELNPITSE EVKQVLADLG
510 520 530 540 550
LDTRNTILGH VQRGGTAVAF DRRLATLQGV EAVKAVLEMT PDTPSPMIGI
560 570 580 590 600
LKHKIVRIPL VDAVKQTKAV AEAISNKDFD KAMSLRDNSF YDDYRYFRDI
610 620 630 640 650
SIYDDGSKQL SEDKRLNIAI VHVGAASAGL NAATRAVALY SLSRGHKLYA
660 670 680 690 700
VQDGFAGLVK GDLKNLTWMD VEGWHSLGGS EIGTNRSLPS QNIGKVAYNL
710 720 730 740 750
QKFNIQGLLI VGGFEAFTSL HELSEQKANY PIFEIPMVVV PATVSNNVPG
760 770 780 790 800
TEYSLGADTC LNQLVSYCDA VQQSASSTRR RVFVVEVQGG HSGYVASYCG
810 820 830 840 850
LITGALATYT PESNINLREL QGDIDLLQKV FATDRGEDHN GTLIVRNEQA
860 870 880 890 900
SAVYSTQLIA DILKENANKR FETRTAIPGH VQQGFTPSAN DRVMAVKFSL
910 920 930 940 950
KAMEFIETRN GCYGKHDRKF SDEEISEHSQ VVIGIHGDVV KFTCIKHLYD
960 970 980
NEANVALRKG KTVHWTDMID VANILNGKSL LKKQERY
Length:987
Mass (Da):108,598
Last modified:May 1, 1999 - v1
Checksum:i4EE5BAB6D02349FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007638 Genomic DNA. Translation: CAB38868.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007638 Genomic DNA. Translation: CAB38868.1 .

3D structure databases

ProteinModelPortali O94201.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0205.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans."
    Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J.
    Eur. J. Biochem. 260:217-226(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594.

Entry informationi

Entry nameiPFKA1_CANAX
AccessioniPrimary (citable) accession number: O94201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3