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O94200

- PFKA2_CANAX

UniProt

O94200 - PFKA2_CANAX

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Protein
ATP-dependent 6-phosphofructokinase subunit beta
Gene
PFK2
Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921ATP; via amide nitrogen By similarity
Metal bindingi287 – 2871Magnesium; catalytic By similarity
Active sitei334 – 3341Proton acceptor By similarity
Binding sitei369 – 3691Substrate; shared with subunit alpha By similarity
Binding sitei433 – 4331Substrate By similarity
Binding sitei461 – 4611Substrate; shared with subunit alpha By similarity
Binding sitei644 – 6441Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei833 – 8331Allosteric activator fructose 2,6-bisphosphate; shared with subunit alpha By similarity
Binding sitei920 – 9201Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi256 – 2572ATP By similarity
Nucleotide bindingi286 – 2894ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit beta (EC:2.7.1.11)
Alternative name(s):
ATP-dependent 6-phosphofructokinase 2
Short name:
ATP-PFK 2
Short name:
Phosphofructokinase 2
Phosphohexokinase 2
Gene namesi
Name:PFK2
OrganismiCandida albicans (Yeast)
Taxonomic identifieri5476 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946ATP-dependent 6-phosphofructokinase subunit betaUniRule annotation
PRO_0000112039Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains By similarity.

Structurei

3D structure databases

ProteinModelPortaliO94200.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 559559N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni332 – 3343Substrate binding By similarity
Regioni376 – 3783Substrate binding By similarity
Regioni467 – 4704Substrate binding By similarity
Regioni560 – 57314Interdomain linkerUniRule annotation
Add
BLAST
Regioni574 – 946373C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni702 – 7065Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni747 – 7493Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni839 – 8424Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
SSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94200-1 [UniParc]FASTAAdd to Basket

« Hide

MISIVNGTST LSLVAGSVET LNQAINFYTN ILGLSVHSEQ NDWTYLSNDD    50
NKMIVKIQLD TKSGLSLDQV NDRRTEIIAK LNVTDWRSLD TTSVLKVQNL 100
VALIETLTTF NYTLQITPNE LYPNEVYCVG PIGYIIGFTA CDEPLTLVPP 150
LQKSHPKPGL VSNLMSKSGS QSRNIEETKA VRRNIAVMTS GGDSQGMNAA 200
VRAVVRATIF HGSKAFAVQE GYAGLVKGGP EYIKEMKWQD VRGFLSEGGT 250
NIGTARCMEF KERWGRLKGC KNLIDAGIDG LIVCGGDGSL TGADLFRHEW 300
PSLIQELKDK GEITNEQFER HKHLYICGMV GSIDNDMAMT DATIGGYSAL 350
ERICRAIDYI DATANSHSRA FVVEVMGRHC GWLALMAGIA TSADYIFIPE 400
KPASSKDWQD QMCDIVGKHR AQGKRKTIVI VAEGAITSDL KPITSDEVKD 450
VLVDRLGLDT RITVLGHVQR GGTAVAFDRT LATLQGVEAV KAILELTPDV 500
PSPLIAIDEN KICRRPLVEA VRITKSVASA IEAKDFEKAM SLRDHEFKEH 550
LANFMAMNTA NHEKPTLPRE KRKKIAIINI GAPAGGMNSA VYAMATYCMS 600
RGHTPYAIHN GFAGLSRHES VKSIEWIDIE GWNSIGGSEI GTNRQTPEET 650
DIGMIAHYFE KYQFDGLIIV GGFEAFVSLE QLERSRAMYP SFRIPMVLIP 700
ATISNNVPGT EYSLGADTCL NSLMEYCDIV KQSASATRGT AFIIDVQGGN 750
SGYIATFASL ISGAQASYVP EEGISLQQLE MDINSLREAF AVEQGMTKSG 800
KLIIKSSNAS KVLTPHTLAD IFNDECHGDF DTKTAIPGHV QQGGLPSPID 850
RSRGDRFAIR AVQFIEDHCD VLAPYRYELD FPIDDKKILN TAAVLGIKSS 900
RLRFTSIRHL FDFETELGRR MPKTIYWNTI RDISDQLVGR TRLDKP 946
Length:946
Mass (Da):104,046
Last modified:May 1, 1999 - v1
Checksum:iAEF6167A741D8672
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007637 Genomic DNA. Translation: CAB38867.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007637 Genomic DNA. Translation: CAB38867.1 .

3D structure databases

ProteinModelPortali O94200.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0205.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

Gene3Di 3.10.180.10. 1 hit.
HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
SSF54593. SSF54593. 1 hit.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans."
    Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J.
    Eur. J. Biochem. 260:217-226(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594.

Entry informationi

Entry nameiPFKA2_CANAX
AccessioniPrimary (citable) accession number: O94200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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