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O94200

- PFKA2_CANAX

UniProt

O94200 - PFKA2_CANAX

Protein

ATP-dependent 6-phosphofructokinase subunit beta

Gene

PFK2

Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei192 – 1921ATP; via amide nitrogenUniRule annotation
    Metal bindingi287 – 2871Magnesium; catalyticUniRule annotation
    Active sitei334 – 3341Proton acceptorUniRule annotation
    Binding sitei369 – 3691Substrate; shared with subunit alphaUniRule annotation
    Binding sitei433 – 4331SubstrateUniRule annotation
    Binding sitei461 – 4611Substrate; shared with subunit alphaUniRule annotation
    Binding sitei644 – 6441Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei833 – 8331Allosteric activator fructose 2,6-bisphosphate; shared with subunit alphaUniRule annotation
    Binding sitei920 – 9201Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi256 – 2572ATPUniRule annotation
    Nucleotide bindingi286 – 2894ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase subunit betaUniRule annotation (EC:2.7.1.11UniRule annotation)
    Alternative name(s):
    ATP-dependent 6-phosphofructokinase 2UniRule annotation
    Short name:
    ATP-PFK 2UniRule annotation
    Short name:
    Phosphofructokinase 2UniRule annotation
    Phosphohexokinase 2UniRule annotation
    Gene namesi
    Name:PFK2
    OrganismiCandida albicans (Yeast)
    Taxonomic identifieri5476 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 946946ATP-dependent 6-phosphofructokinase subunit betaPRO_0000112039Add
    BLAST

    Interactioni

    Subunit structurei

    Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliO94200.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 559559N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni332 – 3343Substrate bindingUniRule annotation
    Regioni376 – 3783Substrate bindingUniRule annotation
    Regioni467 – 4704Substrate bindingUniRule annotation
    Regioni560 – 57314Interdomain linkerAdd
    BLAST
    Regioni574 – 946373C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni702 – 7065Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni747 – 7493Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni839 – 8424Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.

    Family and domain databases

    Gene3Di3.10.180.10. 1 hit.
    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    SSF54593. SSF54593. 1 hit.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94200-1 [UniParc]FASTAAdd to Basket

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    MISIVNGTST LSLVAGSVET LNQAINFYTN ILGLSVHSEQ NDWTYLSNDD    50
    NKMIVKIQLD TKSGLSLDQV NDRRTEIIAK LNVTDWRSLD TTSVLKVQNL 100
    VALIETLTTF NYTLQITPNE LYPNEVYCVG PIGYIIGFTA CDEPLTLVPP 150
    LQKSHPKPGL VSNLMSKSGS QSRNIEETKA VRRNIAVMTS GGDSQGMNAA 200
    VRAVVRATIF HGSKAFAVQE GYAGLVKGGP EYIKEMKWQD VRGFLSEGGT 250
    NIGTARCMEF KERWGRLKGC KNLIDAGIDG LIVCGGDGSL TGADLFRHEW 300
    PSLIQELKDK GEITNEQFER HKHLYICGMV GSIDNDMAMT DATIGGYSAL 350
    ERICRAIDYI DATANSHSRA FVVEVMGRHC GWLALMAGIA TSADYIFIPE 400
    KPASSKDWQD QMCDIVGKHR AQGKRKTIVI VAEGAITSDL KPITSDEVKD 450
    VLVDRLGLDT RITVLGHVQR GGTAVAFDRT LATLQGVEAV KAILELTPDV 500
    PSPLIAIDEN KICRRPLVEA VRITKSVASA IEAKDFEKAM SLRDHEFKEH 550
    LANFMAMNTA NHEKPTLPRE KRKKIAIINI GAPAGGMNSA VYAMATYCMS 600
    RGHTPYAIHN GFAGLSRHES VKSIEWIDIE GWNSIGGSEI GTNRQTPEET 650
    DIGMIAHYFE KYQFDGLIIV GGFEAFVSLE QLERSRAMYP SFRIPMVLIP 700
    ATISNNVPGT EYSLGADTCL NSLMEYCDIV KQSASATRGT AFIIDVQGGN 750
    SGYIATFASL ISGAQASYVP EEGISLQQLE MDINSLREAF AVEQGMTKSG 800
    KLIIKSSNAS KVLTPHTLAD IFNDECHGDF DTKTAIPGHV QQGGLPSPID 850
    RSRGDRFAIR AVQFIEDHCD VLAPYRYELD FPIDDKKILN TAAVLGIKSS 900
    RLRFTSIRHL FDFETELGRR MPKTIYWNTI RDISDQLVGR TRLDKP 946
    Length:946
    Mass (Da):104,046
    Last modified:May 1, 1999 - v1
    Checksum:iAEF6167A741D8672
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ007637 Genomic DNA. Translation: CAB38867.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ007637 Genomic DNA. Translation: CAB38867.1 .

    3D structure databases

    ProteinModelPortali O94200.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0205.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    Gene3Di 3.10.180.10. 1 hit.
    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    SSF54593. SSF54593. 1 hit.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans."
      Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J.
      Eur. J. Biochem. 260:217-226(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594.

    Entry informationi

    Entry nameiPFKA2_CANAX
    AccessioniPrimary (citable) accession number: O94200
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3