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O94200

- PFKA2_CANAX

UniProt

O94200 - PFKA2_CANAX

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Protein

ATP-dependent 6-phosphofructokinase subunit beta

Gene

PFK2

Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921ATP; via amide nitrogenUniRule annotation
Metal bindingi287 – 2871Magnesium; catalyticUniRule annotation
Active sitei334 – 3341Proton acceptorUniRule annotation
Binding sitei369 – 3691Substrate; shared with subunit alphaUniRule annotation
Binding sitei433 – 4331SubstrateUniRule annotation
Binding sitei461 – 4611Substrate; shared with subunit alphaUniRule annotation
Binding sitei644 – 6441Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei833 – 8331Allosteric activator fructose 2,6-bisphosphate; shared with subunit alphaUniRule annotation
Binding sitei920 – 9201Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi256 – 2572ATPUniRule annotation
Nucleotide bindingi286 – 2894ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit betaUniRule annotation (EC:2.7.1.11UniRule annotation)
Alternative name(s):
ATP-dependent 6-phosphofructokinase 2UniRule annotation
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:PFK2
OrganismiCandida albicans (Yeast)
Taxonomic identifieri5476 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946ATP-dependent 6-phosphofructokinase subunit betaPRO_0000112039Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO94200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 559559N-terminal catalytic PFK domain 1Add
BLAST
Regioni332 – 3343Substrate bindingUniRule annotation
Regioni376 – 3783Substrate bindingUniRule annotation
Regioni467 – 4704Substrate bindingUniRule annotation
Regioni560 – 57314Interdomain linkerAdd
BLAST
Regioni574 – 946373C-terminal regulatory PFK domain 2Add
BLAST
Regioni702 – 7065Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni747 – 7493Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni839 – 8424Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
SSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94200-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISIVNGTST LSLVAGSVET LNQAINFYTN ILGLSVHSEQ NDWTYLSNDD
60 70 80 90 100
NKMIVKIQLD TKSGLSLDQV NDRRTEIIAK LNVTDWRSLD TTSVLKVQNL
110 120 130 140 150
VALIETLTTF NYTLQITPNE LYPNEVYCVG PIGYIIGFTA CDEPLTLVPP
160 170 180 190 200
LQKSHPKPGL VSNLMSKSGS QSRNIEETKA VRRNIAVMTS GGDSQGMNAA
210 220 230 240 250
VRAVVRATIF HGSKAFAVQE GYAGLVKGGP EYIKEMKWQD VRGFLSEGGT
260 270 280 290 300
NIGTARCMEF KERWGRLKGC KNLIDAGIDG LIVCGGDGSL TGADLFRHEW
310 320 330 340 350
PSLIQELKDK GEITNEQFER HKHLYICGMV GSIDNDMAMT DATIGGYSAL
360 370 380 390 400
ERICRAIDYI DATANSHSRA FVVEVMGRHC GWLALMAGIA TSADYIFIPE
410 420 430 440 450
KPASSKDWQD QMCDIVGKHR AQGKRKTIVI VAEGAITSDL KPITSDEVKD
460 470 480 490 500
VLVDRLGLDT RITVLGHVQR GGTAVAFDRT LATLQGVEAV KAILELTPDV
510 520 530 540 550
PSPLIAIDEN KICRRPLVEA VRITKSVASA IEAKDFEKAM SLRDHEFKEH
560 570 580 590 600
LANFMAMNTA NHEKPTLPRE KRKKIAIINI GAPAGGMNSA VYAMATYCMS
610 620 630 640 650
RGHTPYAIHN GFAGLSRHES VKSIEWIDIE GWNSIGGSEI GTNRQTPEET
660 670 680 690 700
DIGMIAHYFE KYQFDGLIIV GGFEAFVSLE QLERSRAMYP SFRIPMVLIP
710 720 730 740 750
ATISNNVPGT EYSLGADTCL NSLMEYCDIV KQSASATRGT AFIIDVQGGN
760 770 780 790 800
SGYIATFASL ISGAQASYVP EEGISLQQLE MDINSLREAF AVEQGMTKSG
810 820 830 840 850
KLIIKSSNAS KVLTPHTLAD IFNDECHGDF DTKTAIPGHV QQGGLPSPID
860 870 880 890 900
RSRGDRFAIR AVQFIEDHCD VLAPYRYELD FPIDDKKILN TAAVLGIKSS
910 920 930 940
RLRFTSIRHL FDFETELGRR MPKTIYWNTI RDISDQLVGR TRLDKP
Length:946
Mass (Da):104,046
Last modified:May 1, 1999 - v1
Checksum:iAEF6167A741D8672
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007637 Genomic DNA. Translation: CAB38867.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007637 Genomic DNA. Translation: CAB38867.1 .

3D structure databases

ProteinModelPortali O94200.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0205.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

Gene3Di 3.10.180.10. 1 hit.
HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
SSF54593. SSF54593. 1 hit.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans."
    Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J.
    Eur. J. Biochem. 260:217-226(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594.

Entry informationi

Entry nameiPFKA2_CANAX
AccessioniPrimary (citable) accession number: O94200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3