O94200 (K6PF2_CANAX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase subunit beta
EC=2.7.1.11

Alternative name(s):
6PF-1-K subunit beta
CaPFK2
Phosphofructokinase 2
Phosphohexokinase

Gene names

Name:

PFK2

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	6-phosphofructokinase subunit beta		PRO_0000112039
Regions
	Nucleotide binding	202 – 206	5	ATP By similarity
	Nucleotide binding	361 – 365	5	ATP By similarity
	Nucleotide binding	378 – 394	17	ATP By similarity
Sites
	Active site	334	1	Proton acceptor By similarity
	Binding site	369	1	Substrate By similarity
	Binding site	461	1	Substrate By similarity
	Binding site	467	1	Substrate By similarity
	Binding site	470	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94200 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: AEF6167A741D8672	FASTA	946	104,046
	10 20 30 40 50 60 MISIVNGTST LSLVAGSVET LNQAINFYTN ILGLSVHSEQ NDWTYLSNDD NKMIVKIQLD 70 80 90 100 110 120 TKSGLSLDQV NDRRTEIIAK LNVTDWRSLD TTSVLKVQNL VALIETLTTF NYTLQITPNE 130 140 150 160 170 180 LYPNEVYCVG PIGYIIGFTA CDEPLTLVPP LQKSHPKPGL VSNLMSKSGS QSRNIEETKA 190 200 210 220 230 240 VRRNIAVMTS GGDSQGMNAA VRAVVRATIF HGSKAFAVQE GYAGLVKGGP EYIKEMKWQD 250 260 270 280 290 300 VRGFLSEGGT NIGTARCMEF KERWGRLKGC KNLIDAGIDG LIVCGGDGSL TGADLFRHEW 310 320 330 340 350 360 PSLIQELKDK GEITNEQFER HKHLYICGMV GSIDNDMAMT DATIGGYSAL ERICRAIDYI 370 380 390 400 410 420 DATANSHSRA FVVEVMGRHC GWLALMAGIA TSADYIFIPE KPASSKDWQD QMCDIVGKHR 430 440 450 460 470 480 AQGKRKTIVI VAEGAITSDL KPITSDEVKD VLVDRLGLDT RITVLGHVQR GGTAVAFDRT 490 500 510 520 530 540 LATLQGVEAV KAILELTPDV PSPLIAIDEN KICRRPLVEA VRITKSVASA IEAKDFEKAM 550 560 570 580 590 600 SLRDHEFKEH LANFMAMNTA NHEKPTLPRE KRKKIAIINI GAPAGGMNSA VYAMATYCMS 610 620 630 640 650 660 RGHTPYAIHN GFAGLSRHES VKSIEWIDIE GWNSIGGSEI GTNRQTPEET DIGMIAHYFE 670 680 690 700 710 720 KYQFDGLIIV GGFEAFVSLE QLERSRAMYP SFRIPMVLIP ATISNNVPGT EYSLGADTCL 730 740 750 760 770 780 NSLMEYCDIV KQSASATRGT AFIIDVQGGN SGYIATFASL ISGAQASYVP EEGISLQQLE 790 800 810 820 830 840 MDINSLREAF AVEQGMTKSG KLIIKSSNAS KVLTPHTLAD IFNDECHGDF DTKTAIPGHV 850 860 870 880 890 900 QQGGLPSPID RSRGDRFAIR AVQFIEDHCD VLAPYRYELD FPIDDKKILN TAAVLGIKSS 910 920 930 940 RLRFTSIRHL FDFETELGRR MPKTIYWNTI RDISDQLVGR TRLDKP

References

[1]	"Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans." Lorberg A., Kirchrath L., Ernst J.F., Heinisch J.J. Eur. J. Biochem. 260:217-226(1999) [PubMed: 10091602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ007637 Genomic DNA. Translation: CAB38867.1.
3D structure databases
ProteinModelPortal	O94200.
ModBase	Search...
Protein-protein interaction databases
STRING	O94200.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF2_CANAX

Accession

Primary (citable) accession number: O94200

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	May 1, 1999
Last modified:	October 19, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program