O94198 (ACEA_ASHGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	ICL1
Ordered Locus Names:	ADL066C

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii) [Reference proteome]

Taxonomic identifier

284811 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Eremothecium › Ashbya gossypii

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Peroxisome
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Isocitrate lyase		PRO_0000068782
Regions
	Motif	558 – 560	3	Microbody targeting signal Potential
Sites
	Active site	217	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94198 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6E9FCA3A608438AD	FASTA	560	62,585
	10 20 30 40 50 60 MSPSVRDARN DLASLQQQAA AEAEDIRRWW SQPRWAGTKR VYTAEDIVKR RGTFPVVEYP 70 80 90 100 110 120 SSVMADKLVE TLARHSRNGT VSQTFGVLDP VQMTQMVKYL DTIYVSGWQC SATASTSNEP 130 140 150 160 170 180 GPDLADYPMD TVPNKVEHLF MAQLFHDRKQ REARLSCTTQ RELDQLGPEI DYLRPIVADA 190 200 210 220 230 240 DTGHGGLTAV FKLTKMFIER GAAGIHMEDQ SSSNKKCGHM AGRCVIPVQE HISRLVTVRM 250 260 270 280 290 300 CADVMHSNLV LVARTDSEAA TLLSSNIDAR DHYYIVGASN PEVTVPLIEV LDAAQQAGAS 310 320 330 340 350 360 GDRLAQLEED WCKKAKLRLF HEAFADQVNA SPSIKDKAGV IAKFNSQIGP QTGASIREMR 370 380 390 400 410 420 KLGRELLGQD VYFDWDLPRA REGLYRYKGG TQCAIMRARA FAPYADLVWF ESNFPDFQQA 430 440 450 460 470 480 KEFAQGVREK FPNKWMAYNL SPSFNWPKAM PPKEQENYIQ RLGEIGYVWQ FITLAGLHTN 490 500 510 520 530 540 ALAIDNFSRE FSRFGMRAYA QGIQQREMDE GVDVLKHQKW AGAEYVDSIL KLAQGGVSST 550 560 ASMGKGVTEE QFGSSNGAKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isocitrate lyase of Ashbya gossypii -- transcriptional regulation and peroxisomal localization." Maeting I., Schmidt G., Sahm H., Revuelta J.L., Stierhof Y.D., Stahmann K.-P. FEBS Lett. 444:15-21(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.
[2]	"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome." Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P. Science 304:304-307(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ010727 Genomic DNA. Translation: CAB37065.1. AE016817 Genomic DNA. Translation: AAS51854.1.
RefSeq	NP_984030.1. NM_209383.1.
3D structure databases
ProteinModelPortal	O94198.
SMR	O94198. Positions 18-536.
ModBase	Search...
Protein-protein interaction databases
STRING	33169.AGOS_ADL066C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	AAS51854; AAS51854; AGOS_ADL066C.
GeneID	4620172.
KEGG	ago:AGOS_ADL066C.
Phylogenomic databases
eggNOG	COG2224.
HOGENOM	HOG000238475.
KO	K01637.
OMA	MVTQMAK.
OrthoDB	EOG4P5PJP.
PhylomeDB	O94198.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_ASHGO

Accession

Primary (citable) accession number: O94198

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program