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O94163

- XYNF1_ASPOR

UniProt

O94163 - XYNF1_ASPOR

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Protein
Endo-1,4-beta-xylanase F1
Gene
xynF1, xlnF1, AO090103000423
Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

pH dependencei

Optimum pH is 5.0.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551Proton donor By similarity
Active sitei263 – 2631Nucleophile By similarity

GO - Molecular functioni

  1. endo-1,4-beta-xylanase activity Source: UniProtKB

GO - Biological processi

  1. xylan catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiGH10. Glycoside Hydrolase Family 10.
mycoCLAPiXYN10F_ASPOR.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase F1 (EC:3.2.1.8)
Short name:
Xylanase F1
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase F1
Gene namesi
Name:xynF1
Synonyms:xlnF1
ORF Names:AO090103000423
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564: Chromosome 8

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: ASPGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 327308Endo-1,4-beta-xylanase F1
PRO_0000393191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi281 ↔ 287 By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi5062.CADAORAP00010097.

Structurei

3D structure databases

ProteinModelPortaliO94163.
SMRiO94163. Positions 28-327.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3693.
HOGENOMiHOG000019847.
OrthoDBiEOG7GJ6PM.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O94163-1 [UniParc]FASTAAdd to Basket

« Hide

MVHLKALASG TLFASLASSA VISRQAAASI NDAFVAHGKK YFGTCSDQAL    50
LQNSQNEAIV RADFGQLTPE NSMKWDALEP SQGSFSFAGA DFLADYAKTN 100
NKLVRGHTLV WHSQLPSWVQ GITDKDTLTE VIKNHITTIM QRYKGQIYAW 150
DVVNEIFDED GTLRDSVFSQ VLGEDFVRIA FETAREADPN AKLYINDYNL 200
DSADYAKTKG MVSYVKKWLD AGVPIDGIGS QSHYSANGFP VSGAKGALTA 250
LASTGVSEVA VTELDIEGAS SESYLEVVNA CLDVSSCVGI TVWGVSDKDS 300
WRSSTSPLLF DSNYQAKDAY NAIIDAL 327
Length:327
Mass (Da):35,402
Last modified:May 1, 1999 - v1
Checksum:i38B2F6374BAD3D0A
GO

Sequence cautioni

The sequence BAE65852.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901N → D in BAE65852. 1 Publication
Sequence conflicti209 – 2091K → Q in BAE65852. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011212 Genomic DNA. Translation: BAA75475.1.
AP007174 Genomic DNA. Translation: BAE65852.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011212 Genomic DNA. Translation: BAA75475.1 .
AP007174 Genomic DNA. Translation: BAE65852.1 . Sequence problems.

3D structure databases

ProteinModelPortali O94163.
SMRi O94163. Positions 28-327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5062.CADAORAP00010097.

Protein family/group databases

CAZyi GH10. Glycoside Hydrolase Family 10.
mycoCLAPi XYN10F_ASPOR.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3693.
HOGENOMi HOG000019847.
OrthoDBi EOG7GJ6PM.

Enzyme and pathway databases

UniPathwayi UPA00114 .

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00331. Glyco_hydro_10. 1 hit.
[Graphical view ]
PRINTSi PR00134. GLHYDRLASE10.
SMARTi SM00633. Glyco_10. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Repression of the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae by introduction of multiple copies of the xynF1 promoter."
    Kitamoto N., Yoshino S., Ito M., Kimura T., Ohmiya K., Tsukagoshi N.
    Appl. Microbiol. Biotechnol. 50:558-563(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KBN616.
  2. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.
  3. "Purification and characterization of the overexpressed Aspergillus oryzae xylanase, XynF1."
    Kitamoto N., Yoshino S., Ohmiya K., Tsukagoshi N.
    Biosci. Biotechnol. Biochem. 63:1791-1794(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiXYNF1_ASPOR
AccessioniPrimary (citable) accession number: O94163
Secondary accession number(s): Q2TY21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: May 1, 1999
Last modified: February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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