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O94163

- XYNF1_ASPOR

UniProt

O94163 - XYNF1_ASPOR

Protein

Endo-1,4-beta-xylanase F1

Gene

xynF1

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

    pH dependencei

    Optimum pH is 5.0.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei155 – 1551Proton donorBy similarity
    Active sitei263 – 2631NucleophileBy similarity

    GO - Molecular functioni

    1. endo-1,4-beta-xylanase activity Source: UniProtKB

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiGH10. Glycoside Hydrolase Family 10.
    mycoCLAPiXYN10F_ASPOR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase F1 (EC:3.2.1.8)
    Short name:
    Xylanase F1
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase F1
    Gene namesi
    Name:xynF1
    Synonyms:xlnF1
    ORF Names:AO090103000423
    OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
    Taxonomic identifieri510516 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006564: Chromosome 8

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: ASPGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 327308Endo-1,4-beta-xylanase F1PRO_0000393191Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi281 ↔ 287By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi5062.CADAORAP00010097.

    Structurei

    3D structure databases

    ProteinModelPortaliO94163.
    SMRiO94163. Positions 28-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3693.
    HOGENOMiHOG000019847.
    OrthoDBiEOG7GJ6PM.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00331. Glyco_hydro_10. 1 hit.
    [Graphical view]
    PRINTSiPR00134. GLHYDRLASE10.
    SMARTiSM00633. Glyco_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O94163-1 [UniParc]FASTAAdd to Basket

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    MVHLKALASG TLFASLASSA VISRQAAASI NDAFVAHGKK YFGTCSDQAL    50
    LQNSQNEAIV RADFGQLTPE NSMKWDALEP SQGSFSFAGA DFLADYAKTN 100
    NKLVRGHTLV WHSQLPSWVQ GITDKDTLTE VIKNHITTIM QRYKGQIYAW 150
    DVVNEIFDED GTLRDSVFSQ VLGEDFVRIA FETAREADPN AKLYINDYNL 200
    DSADYAKTKG MVSYVKKWLD AGVPIDGIGS QSHYSANGFP VSGAKGALTA 250
    LASTGVSEVA VTELDIEGAS SESYLEVVNA CLDVSSCVGI TVWGVSDKDS 300
    WRSSTSPLLF DSNYQAKDAY NAIIDAL 327
    Length:327
    Mass (Da):35,402
    Last modified:May 1, 1999 - v1
    Checksum:i38B2F6374BAD3D0A
    GO

    Sequence cautioni

    The sequence BAE65852.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti190 – 1901N → D in BAE65852. (PubMed:16372010)Curated
    Sequence conflicti209 – 2091K → Q in BAE65852. (PubMed:16372010)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011212 Genomic DNA. Translation: BAA75475.1.
    AP007174 Genomic DNA. Translation: BAE65852.1. Sequence problems.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011212 Genomic DNA. Translation: BAA75475.1 .
    AP007174 Genomic DNA. Translation: BAE65852.1 . Sequence problems.

    3D structure databases

    ProteinModelPortali O94163.
    SMRi O94163. Positions 28-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5062.CADAORAP00010097.

    Protein family/group databases

    CAZyi GH10. Glycoside Hydrolase Family 10.
    mycoCLAPi XYN10F_ASPOR.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG3693.
    HOGENOMi HOG000019847.
    OrthoDBi EOG7GJ6PM.

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001000. Glyco_hydro_10.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00331. Glyco_hydro_10. 1 hit.
    [Graphical view ]
    PRINTSi PR00134. GLHYDRLASE10.
    SMARTi SM00633. Glyco_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Repression of the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae by introduction of multiple copies of the xynF1 promoter."
      Kitamoto N., Yoshino S., Ito M., Kimura T., Ohmiya K., Tsukagoshi N.
      Appl. Microbiol. Biotechnol. 50:558-563(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KBN616.
    2. "Genome sequencing and analysis of Aspergillus oryzae."
      Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
      , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
      Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.
    3. "Purification and characterization of the overexpressed Aspergillus oryzae xylanase, XynF1."
      Kitamoto N., Yoshino S., Ohmiya K., Tsukagoshi N.
      Biosci. Biotechnol. Biochem. 63:1791-1794(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiXYNF1_ASPOR
    AccessioniPrimary (citable) accession number: O94163
    Secondary accession number(s): Q2TY21
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 23, 2010
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3