ID   KO1_GIBFU               Reviewed;         525 AA.
AC   O94142;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   24-MAR-2009, entry version 46.
DE   RecName: Full=Ent-kaurene oxidase;
DE            EC=1.14.13.78;
DE   AltName: Full=Cytochrome P450-4;
DE   AltName: Full=Cytochrome P450 503A1;
GN   Name=CYP503A1;
OS   Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium
OS   moniliforme).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae;
OC   Gibberella; Gibberella fujikuroi complex.
OX   NCBI_TaxID=5127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=m567;
RX   PubMed=11472927; DOI=10.1128/AEM.67.8.3514-3522.2001;
RA   Tudzynski B., Hedden P., Carrera E., Gaskin P.;
RT   "The P450-4 gene of Gibberella fujikuroi encodes ent-kaurene oxidase
RT   in the gibberellin biosynthesis pathway.";
RL   Appl. Environ. Microbiol. 67:3514-3522(2001).
RN   [2]
RP   INDUCTION.
RX   PubMed=12581353; DOI=10.1046/j.1365-2958.2003.03326.x;
RA   Mihlan M., Homann V., Liu T.-W.D., Tudzynski B.;
RT   "AREA directly mediates nitrogen regulation of gibberellin
RT   biosynthesis in Gibberella fujikuroi, but its activity is not affected
RT   by NMR.";
RL   Mol. Microbiol. 47:975-991(2003).
CC   -!- FUNCTION: Catalyzes three successive oxidations of the 4-methyl
CC       group of ent-kaurene giving kaurenoic acid, a key step in
CC       gibberellin (GA) biosynthesis.
CC   -!- CATALYTIC ACTIVITY: Ent-kaur-16-ene + NADPH + O(2) = ent-kaur-16-
CC       en-19-ol + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: Ent-kaur-16-en-19-ol + NADPH + O(2) = ent-
CC       kaur-16-en-19-al + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: Ent-kaur-16-en-19-al + NADPH + O(2) = ent-
CC       kaur-16-en-19-oate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- INDUCTION: By nitrogen starvation.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; Y17243; CAA76703.1; -; Genomic_DNA.
DR   BioCyc; MetaCyc:MON-11667; -.
DR   BRENDA; 1.14.13.78; 97815.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0010241; F:ent-kaurene oxidase activity; IEA:EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006950; P:response to stress; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
PE   1: Evidence at protein level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP;
KW   Oxidoreductase; Stress response; Transmembrane.
FT   CHAIN         1    525       Ent-kaurene oxidase.
FT                                /FTId=PRO_0000052208.
FT   TRANSMEM     31     51       Potential.
FT   METAL       466    466       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   525 AA;  59951 MW;  CE6DEC6788AC61B7 CRC64;
     MSKSNSMNST SHETLFQQLV LGLDRMPLMD VHWLIYVAFG AWLCSYVIHV LSSSSTVKVP
     VVGYRSVFEP TWLLRLRFVW EGGSIIGQGY NKFKDSIFQV RKLGTDIVII PPNYIDEVRK
     LSQDKTRSVE PFINDFAGQY TRGMVFLQSD LQNRVIQQRL TPKLVSLTKV MKEELDYALT
     KEMPDMKNDE WVEVDISSIM VRLISRISAR VFLGPEHCRN QEWLTTTAEY SESLFITGFI
     LRVVPHILRP FIAPLLPSYR TLLRNVSSGR RVIGDIIRSQ QGDGNEDILS WMRDAATGEE
     KQIDNIAQRM LILSLASIHT TAMTMTHAMY DLCACPEYIE PLRDEVKSVV GASGWDKTAL
     NRFHKLDSFL KESQRFNPVF LLTFNRIYHQ SMTLSDGTNI PSGTRIAVPS HAMLQDSAHV
     PGPTPPTEFD GFRYSKIRSD SNYAQKYLFS MTDSSNMAFG YGKYACPGRF YASNEMKLTL
     AILLLQFEFK LPDGKGRPRN ITIDSDMIPD PRARLCVRKR SLRDE
//