Reviewed, UniProtKB/Swiss-Prot O94142 (KO1_GIBFU)

Names and origin

Protein names

Recommended name:
    Ent-kaurene oxidase
    EC=1.14.13.78
Alternative name(s):
    Cytochrome P450-4
    Cytochrome P450 503A1

Gene names

Name:

CYP503A1

Organism

Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium moniliforme)

Taxonomic identifier

5127 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella › Gibberella fujikuroi complex

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid, a key step in gibberellin (GA) biosynthesis.
Catalytic activity	Ent-kaur-16-ene + NADPH + O2 = ent-kaur-16-en-19-ol + NADP+ + H2O. Ent-kaur-16-en-19-ol + NADPH + O2 = ent-kaur-16-en-19-al + NADP+ + 2 H2O. Ent-kaur-16-en-19-al + NADPH + O2 = ent-kaur-16-en-19-oate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Induction	By nitrogen starvation. Ref.2
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro ent-kaurene oxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Ent-kaurene oxidase		PRO_0000052208
Regions
	Transmembrane	31 – 51	21	Potential
Sites
	Metal binding	466	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94142-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: CE6DEC6788AC61B7	FASTA	525	59,951
	10 20 30 40 50 60 MSKSNSMNST SHETLFQQLV LGLDRMPLMD VHWLIYVAFG AWLCSYVIHV LSSSSTVKVP 70 80 90 100 110 120 VVGYRSVFEP TWLLRLRFVW EGGSIIGQGY NKFKDSIFQV RKLGTDIVII PPNYIDEVRK 130 140 150 160 170 180 LSQDKTRSVE PFINDFAGQY TRGMVFLQSD LQNRVIQQRL TPKLVSLTKV MKEELDYALT 190 200 210 220 230 240 KEMPDMKNDE WVEVDISSIM VRLISRISAR VFLGPEHCRN QEWLTTTAEY SESLFITGFI 250 260 270 280 290 300 LRVVPHILRP FIAPLLPSYR TLLRNVSSGR RVIGDIIRSQ QGDGNEDILS WMRDAATGEE 310 320 330 340 350 360 KQIDNIAQRM LILSLASIHT TAMTMTHAMY DLCACPEYIE PLRDEVKSVV GASGWDKTAL 370 380 390 400 410 420 NRFHKLDSFL KESQRFNPVF LLTFNRIYHQ SMTLSDGTNI PSGTRIAVPS HAMLQDSAHV 430 440 450 460 470 480 PGPTPPTEFD GFRYSKIRSD SNYAQKYLFS MTDSSNMAFG YGKYACPGRF YASNEMKLTL 490 500 510 520 AILLLQFEFK LPDGKGRPRN ITIDSDMIPD PRARLCVRKR SLRDE

References

[1]	"The P450-4 gene of Gibberella fujikuroi encodes ent-kaurene oxidase in the gibberellin biosynthesis pathway." Tudzynski B., Hedden P., Carrera E., Gaskin P. Appl. Environ. Microbiol. 67:3514-3522(2001) [PubMed: 11472927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: m567.
[2]	"AREA directly mediates nitrogen regulation of gibberellin biosynthesis in Gibberella fujikuroi, but its activity is not affected by NMR." Mihlan M., Homann V., Liu T.-W.D., Tudzynski B. Mol. Microbiol. 47:975-991(2003) [PubMed: 12581353] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17243 Genomic DNA. Translation: CAA76703.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11667.
BRENDA	1.14.13.78. 97815.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
ProtoNet	Search...

Entry information

Entry name

KO1_GIBFU

Accession

Primary (citable) accession number: O94142

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	May 1, 1999
Last modified:	March 24, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)