Reviewed, UniProtKB/Swiss-Prot O94123 (PGK_AGABI)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3

Gene names

Name:	pgkA
Synonyms:	pgk

Organism

Agaricus bisporus (Common mushroom)

Taxonomic identifier

5341 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Agaricaceae › Agaricus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Phosphoglycerate kinase		PRO_0000145873
Regions
	Nucleotide binding	372 – 375	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	63 – 66	4	Substrate binding By similarity
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	219	1	ATP By similarity
	Binding site	312	1	ATP; via carbonyl oxygen By similarity
	Binding site	343	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O94123-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 655B4F35C2C622D4	FASTA	416	44,719
	10 20 30 40 50 60 MSLSNKLSIT DLALTGKRVL IRVDFNSPIQ DGKITNPARI NAALPTIKYA LDNGASKVIL 70 80 90 100 110 120 MSHLGRPDGK AISKYSLKPV ASELEKLLKK PVFFLHDCVG PDIEKAVLEA PEGAVVLLEN 130 140 150 160 170 180 LRFHIEEEGS AKNEEGKKIK ADPSKVTQFR EQLTRLGDVY VNDAFGTAHR AHSSMVGIKL 190 200 210 220 230 240 PRRASGFLVK KELDYFAKAL ENPERPFLAI LGGAKVSDKI QLIENMLDKV NCLVIGGGMA 250 260 270 280 290 300 FTFKKTMQNV AIGNSLFDKP GSEKVAGIVE KAKKNNVEIV FPVDYVVGDK FDANANHKIV 310 320 330 340 350 360 TDDEGVPDGW MGLDVGPKSN ELFREAVLKA KTILWNGPPG VFEFPAFAKG SKVLLDATVE 370 380 390 400 410 AVQKGATVIV GGGDTATVVA NHDAEEKLSH VSTGGGASLE LLEGKTLPGV AELSNK

References

[1]	"Sequence of the Agaricus bisporus pgkA gene." Schaap P.J., Mueller Y., van Griensven L.J.L.D., Visser J. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Horst H39.
[2]	"Biochemical and molecular aspects of growth on fruiting of the edible mushroom Agaricus bisporus." De Groot P.W.J., Visser J., van Griensven L.J.L.D., Schaap P.J. Mycol. Res. 102:1297-1308(1998) [Agricola: IND21972038] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Horst U1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97580 Genomic DNA. Translation: CAA66195.1. X91105 mRNA. Translation: CAA62559.1.
3D structure databases
SMR	O94123. Positions 2-415.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.3. 984.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_AGABI

Accession

Primary (citable) accession number: O94123

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)