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Protein

Pyruvate kinase

Gene

pkiA

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gpd1), Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd2)
  2. Phosphoglycerate kinase (pgkA)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (pkiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63SubstrateBy similarity1
Metal bindingi65PotassiumBy similarity1
Metal bindingi67PotassiumBy similarity1
Metal bindingi99PotassiumBy similarity1
Metal bindingi100Potassium; via carbonyl oxygenBy similarity1
Sitei254Transition state stabilizerBy similarity1
Metal bindingi256MagnesiumSequence analysis1
Binding sitei279Substrate; via amide nitrogenBy similarity1
Metal bindingi280MagnesiumBy similarity1
Binding sitei280Substrate; via amide nitrogenBy similarity1
Binding sitei312SubstrateBy similarity1
Binding sitei351ADPSequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pkiA
OrganismiAgaricus bisporus (White button mushroom)
Taxonomic identifieri5341 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121061 – 532Pyruvate kinaseAdd BLAST532

Proteomic databases

PRIDEiO94122.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO94122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPVDGIRSQ IEWNTTLNVS DAPVPTETTK YHRKTAIIAT IGPKINTVEK
60 70 80 90 100
LTDVRLAGVN IVRMNFSHGT HEYHQSVIDN TRQMIKNDPH GRPVAIALDT
110 120 130 140 150
KGPEIRTGQT RDGNDYPIKA GDEFIVTTDP KYSDICDNKV LFVDYANLAS
160 170 180 190 200
VTAPGKLIYI DDGIISLLIL SIDGMNLHVR ALNNGTLSSR KGVNLPKTDV
210 220 230 240 250
DLPPLSEKDK DDLRFGIRNG VDMIFASFIR RGEDVRQIRE VLGPDGASIK
260 270 280 290 300
IIVKIENEQG VANFDEILKE ADGVMVARGD LGIEIPASQV FLAQKMMIAK
310 320 330 340 350
CNIVGKPVIV ATQMLESMTY NPRPTRAEVS DVANAVLDGS DCVMLSGETA
360 370 380 390 400
KGSYPVQSVL MMAETCLLAE TAICYPPLYD DLRAVQARPT ETAETVAIAA
410 420 430 440 450
VAAAAEQDAK ALLVLSTSGE TARLVSKYRP KIPIITVTRN EQTARQIHLH
460 470 480 490 500
RGCYPFWYPE PRGVQNHQWQ TDVDNRIRFG LRNALALNVI QPGASIIAVQ
510 520 530
GWKGGLGHTN TLRILTVPTD PADLELQPLG SL
Length:532
Mass (Da):58,249
Last modified:May 1, 1999 - v1
Checksum:i3D569B9A2639CDE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97579 Genomic DNA. Translation: CAA66194.1.
X91106 mRNA. Translation: CAA62560.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97579 Genomic DNA. Translation: CAA66194.1.
X91106 mRNA. Translation: CAA62560.1.

3D structure databases

ProteinModelPortaliO94122.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO94122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_AGABI
AccessioniPrimary (citable) accession number: O94122
Secondary accession number(s): Q9UVX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: October 5, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.