Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O94122

- KPYK_AGABI

UniProt

O94122 - KPYK_AGABI

Protein

Pyruvate kinase

Gene

pkiA

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631SubstrateBy similarity
    Metal bindingi65 – 651PotassiumBy similarity
    Metal bindingi67 – 671PotassiumBy similarity
    Metal bindingi99 – 991PotassiumBy similarity
    Metal bindingi100 – 1001Potassium; via carbonyl oxygenBy similarity
    Sitei254 – 2541Transition state stabilizerBy similarity
    Metal bindingi256 – 2561MagnesiumSequence Analysis
    Binding sitei279 – 2791Substrate; via amide nitrogenBy similarity
    Metal bindingi280 – 2801MagnesiumBy similarity
    Binding sitei280 – 2801Substrate; via amide nitrogenBy similarity
    Binding sitei312 – 3121SubstrateBy similarity
    Binding sitei351 – 3511ADPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pkiA
    OrganismiAgaricus bisporus (White button mushroom)
    Taxonomic identifieri5341 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 532532Pyruvate kinasePRO_0000112106Add
    BLAST

    Proteomic databases

    PRIDEiO94122.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO94122.
    SMRiO94122. Positions 33-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O94122-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYPVDGIRSQ IEWNTTLNVS DAPVPTETTK YHRKTAIIAT IGPKINTVEK    50
    LTDVRLAGVN IVRMNFSHGT HEYHQSVIDN TRQMIKNDPH GRPVAIALDT 100
    KGPEIRTGQT RDGNDYPIKA GDEFIVTTDP KYSDICDNKV LFVDYANLAS 150
    VTAPGKLIYI DDGIISLLIL SIDGMNLHVR ALNNGTLSSR KGVNLPKTDV 200
    DLPPLSEKDK DDLRFGIRNG VDMIFASFIR RGEDVRQIRE VLGPDGASIK 250
    IIVKIENEQG VANFDEILKE ADGVMVARGD LGIEIPASQV FLAQKMMIAK 300
    CNIVGKPVIV ATQMLESMTY NPRPTRAEVS DVANAVLDGS DCVMLSGETA 350
    KGSYPVQSVL MMAETCLLAE TAICYPPLYD DLRAVQARPT ETAETVAIAA 400
    VAAAAEQDAK ALLVLSTSGE TARLVSKYRP KIPIITVTRN EQTARQIHLH 450
    RGCYPFWYPE PRGVQNHQWQ TDVDNRIRFG LRNALALNVI QPGASIIAVQ 500
    GWKGGLGHTN TLRILTVPTD PADLELQPLG SL 532
    Length:532
    Mass (Da):58,249
    Last modified:May 1, 1999 - v1
    Checksum:i3D569B9A2639CDE6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97579 Genomic DNA. Translation: CAA66194.1.
    X91106 mRNA. Translation: CAA62560.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97579 Genomic DNA. Translation: CAA66194.1 .
    X91106 mRNA. Translation: CAA62560.1 .

    3D structure databases

    ProteinModelPortali O94122.
    SMRi O94122. Positions 33-517.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O94122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the Agaricus bisporus pkiA gene."
      Schaap P.J., Mueller Y., van Griensven L.J.L.D., Visser J.
      Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Horst H39.
    2. "Biochemical and molecular aspects of growth on fruiting of the edible mushroom Agaricus bisporus."
      De Groot P.W.J., Visser J., van Griensven L.J.L.D., Schaap P.J.
      Mycol. Res. 102:1297-1308(1998)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE OF 3-532.
      Strain: Horst U1.

    Entry informationi

    Entry nameiKPYK_AGABI
    AccessioniPrimary (citable) accession number: O94122
    Secondary accession number(s): Q9UVX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3