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O94122

- KPYK_AGABI

UniProt

O94122 - KPYK_AGABI

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Protein

Pyruvate kinase

Gene

pkiA

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.By similarity
Potassium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631SubstrateBy similarity
Metal bindingi65 – 651PotassiumBy similarity
Metal bindingi67 – 671PotassiumBy similarity
Metal bindingi99 – 991PotassiumBy similarity
Metal bindingi100 – 1001Potassium; via carbonyl oxygenBy similarity
Sitei254 – 2541Transition state stabilizerBy similarity
Metal bindingi256 – 2561MagnesiumSequence Analysis
Binding sitei279 – 2791Substrate; via amide nitrogenBy similarity
Metal bindingi280 – 2801MagnesiumBy similarity
Binding sitei280 – 2801Substrate; via amide nitrogenBy similarity
Binding sitei312 – 3121SubstrateBy similarity
Binding sitei351 – 3511ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pkiA
OrganismiAgaricus bisporus (White button mushroom)
Taxonomic identifieri5341 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Pyruvate kinasePRO_0000112106Add
BLAST

Proteomic databases

PRIDEiO94122.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO94122.
SMRiO94122. Positions 33-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O94122-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYPVDGIRSQ IEWNTTLNVS DAPVPTETTK YHRKTAIIAT IGPKINTVEK
60 70 80 90 100
LTDVRLAGVN IVRMNFSHGT HEYHQSVIDN TRQMIKNDPH GRPVAIALDT
110 120 130 140 150
KGPEIRTGQT RDGNDYPIKA GDEFIVTTDP KYSDICDNKV LFVDYANLAS
160 170 180 190 200
VTAPGKLIYI DDGIISLLIL SIDGMNLHVR ALNNGTLSSR KGVNLPKTDV
210 220 230 240 250
DLPPLSEKDK DDLRFGIRNG VDMIFASFIR RGEDVRQIRE VLGPDGASIK
260 270 280 290 300
IIVKIENEQG VANFDEILKE ADGVMVARGD LGIEIPASQV FLAQKMMIAK
310 320 330 340 350
CNIVGKPVIV ATQMLESMTY NPRPTRAEVS DVANAVLDGS DCVMLSGETA
360 370 380 390 400
KGSYPVQSVL MMAETCLLAE TAICYPPLYD DLRAVQARPT ETAETVAIAA
410 420 430 440 450
VAAAAEQDAK ALLVLSTSGE TARLVSKYRP KIPIITVTRN EQTARQIHLH
460 470 480 490 500
RGCYPFWYPE PRGVQNHQWQ TDVDNRIRFG LRNALALNVI QPGASIIAVQ
510 520 530
GWKGGLGHTN TLRILTVPTD PADLELQPLG SL
Length:532
Mass (Da):58,249
Last modified:May 1, 1999 - v1
Checksum:i3D569B9A2639CDE6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97579 Genomic DNA. Translation: CAA66194.1.
X91106 mRNA. Translation: CAA62560.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97579 Genomic DNA. Translation: CAA66194.1 .
X91106 mRNA. Translation: CAA62560.1 .

3D structure databases

ProteinModelPortali O94122.
SMRi O94122. Positions 33-517.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O94122.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the Agaricus bisporus pkiA gene."
    Schaap P.J., Mueller Y., van Griensven L.J.L.D., Visser J.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Horst H39.
  2. "Biochemical and molecular aspects of growth on fruiting of the edible mushroom Agaricus bisporus."
    De Groot P.W.J., Visser J., van Griensven L.J.L.D., Schaap P.J.
    Mycol. Res. 102:1297-1308(1998)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE OF 3-532.
    Strain: Horst U1.

Entry informationi

Entry nameiKPYK_AGABI
AccessioniPrimary (citable) accession number: O94122
Secondary accession number(s): Q9UVX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: October 1, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3