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O94122 (KPYK_AGABI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40
Gene names
Name:pkiA
OrganismAgaricus bisporus (White button mushroom)
Taxonomic identifier5341 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetidae › Agaricales › Agaricaceae › Agaricus

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 532532Pyruvate kinase
PRO_0000112106

Sites

Metal binding651Potassium By similarity
Metal binding671Potassium By similarity
Metal binding991Potassium By similarity
Metal binding1001Potassium; via carbonyl oxygen By similarity
Metal binding2561Magnesium Potential
Metal binding2801Magnesium By similarity
Binding site631Substrate By similarity
Binding site2791Substrate; via amide nitrogen By similarity
Binding site2801Substrate; via amide nitrogen By similarity
Binding site3121Substrate By similarity
Binding site3511ADP Potential
Site2541Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
O94122 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 3D569B9A2639CDE6

FASTA53258,249
        10         20         30         40         50         60 
MYPVDGIRSQ IEWNTTLNVS DAPVPTETTK YHRKTAIIAT IGPKINTVEK LTDVRLAGVN 

        70         80         90        100        110        120 
IVRMNFSHGT HEYHQSVIDN TRQMIKNDPH GRPVAIALDT KGPEIRTGQT RDGNDYPIKA 

       130        140        150        160        170        180 
GDEFIVTTDP KYSDICDNKV LFVDYANLAS VTAPGKLIYI DDGIISLLIL SIDGMNLHVR 

       190        200        210        220        230        240 
ALNNGTLSSR KGVNLPKTDV DLPPLSEKDK DDLRFGIRNG VDMIFASFIR RGEDVRQIRE 

       250        260        270        280        290        300 
VLGPDGASIK IIVKIENEQG VANFDEILKE ADGVMVARGD LGIEIPASQV FLAQKMMIAK 

       310        320        330        340        350        360 
CNIVGKPVIV ATQMLESMTY NPRPTRAEVS DVANAVLDGS DCVMLSGETA KGSYPVQSVL 

       370        380        390        400        410        420 
MMAETCLLAE TAICYPPLYD DLRAVQARPT ETAETVAIAA VAAAAEQDAK ALLVLSTSGE 

       430        440        450        460        470        480 
TARLVSKYRP KIPIITVTRN EQTARQIHLH RGCYPFWYPE PRGVQNHQWQ TDVDNRIRFG 

       490        500        510        520        530 
LRNALALNVI QPGASIIAVQ GWKGGLGHTN TLRILTVPTD PADLELQPLG SL

« Hide

References

[1]"Sequence of the Agaricus bisporus pkiA gene."
Schaap P.J., Mueller Y., van Griensven L.J.L.D., Visser J.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Horst H39.
[2]"Biochemical and molecular aspects of growth on fruiting of the edible mushroom Agaricus bisporus."
De Groot P.W.J., Visser J., van Griensven L.J.L.D., Schaap P.J.
Mycol. Res. 102:1297-1308(1998) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE OF 3-532.
Strain: Horst U1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X97579 Genomic DNA. Translation: CAA66194.1.
X91106 mRNA. Translation: CAA62560.1.

3D structure databases

ProteinModelPortalO94122.
SMRO94122. Positions 33-517.
ModBaseSearch...

Proteomic databases

PRIDEO94122.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. PK_B_barrel_like. 1 hit.
SSF52935. Pyruvate_kinase. 1 hit.
SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_AGABI
AccessionPrimary (citable) accession number: O94122
Secondary accession number(s): Q9UVX2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: March 6, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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