O93988 (ACL1_SORMK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-citrate synthase subunit 1
EC=2.3.3.8

Alternative name(s):
ATP-citrate (pro-S-)-lyase 1
Citrate cleavage enzyme subunit 1

Gene names

Name:	ACL1
Synonyms:	ACL
ORF Names:	SMAC_06775

Organism

Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) [Complete proteome]

Taxonomic identifier

771870 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Sordaria

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of cytosolic acetyl-CoA, which is mainly used for the biosynthesis of fatty acids and sterols.
Catalytic activity	ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.
Subunit structure	Composed of two subunits.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the succinate/malate CoA ligase alpha subunit family.

Ontologies

Keywords
Biological process	Lipid synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP citrate synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate-CoA ligase (ADP-forming) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	ATP-citrate synthase subunit 1		PRO_0000102787
Regions
	Nucleotide binding	261 – 281	21	ATP By similarity
	Nucleotide binding	312 – 338	27	ATP By similarity
	Region	339 – 349	11	CoA-binding Potential
Sites
	Active site	320	1	Tele-phosphohistidine intermediate By similarity
	Metal binding	278	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	O93988 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 7E75CF2D415E4A61	FASTA	674	72,985
	10 20 30 40 50 60 MPSATSTNGA NGNGNGNGAS ASPAPGNLSA NDNIRRFAAP SRPLSPLPAH ALFNEKTRCF 70 80 90 100 110 120 VYGLQPRAVQ GMLDFDFICK RSTPSVAGII YTFGGQFVSK MYWGTSETLL PVYQEVQKAI 130 140 150 160 170 180 AKHPDVDVVV NFASSRSVYS STMELMEHPQ IKTIAIIAEG VPERRAREIA YVAKKKGITI 190 200 210 220 230 240 IGPATVGGIK PGCFKIGNTG GMMDNIVASK LYRKGSVGYV SKSGGMSNEL NNIISQTTDG 250 260 270 280 290 300 VYEGVAIGGD RYPGTTFIDH LLRYQADPAC KILVLLGEVG GVEEYKVIEA VKQGIITKPI 310 320 330 340 350 360 VAWAIGTCAS MFKTEVQFGH AGAFANSQLE TAATKNKSMR EAGFYVPDTF EDMPALLKQV 370 380 390 400 410 420 YDKLVADGTI VPAPEPVVPK IPIDYSWAQE LGLIRKPAAF ISTISDDRGQ ELLYAGMPIS 430 440 450 460 470 480 DVFREEIGIG GVMSLLWFRR RLPDYAAKFL EMVLMLTADH GPAVSGAMNT IITTRAGKDL 490 500 510 520 530 540 ISSLVAGLLT IGSRFGGALD GAAEEFTKAF DKGLSPREFV DTMRKQNKLI PGIGHRVKSR 550 560 570 580 590 600 NNPDLRVELV KEYVKAKFPS SKLLDYALAV ETVTTSKKDN LILNVDGCIA VCFVDLLRNC 610 620 630 640 650 660 GAFSTEEAED YLSMGVLNGL FVLGRSIGLI AHYLDQKRLR TGLYRHPWDD ITYLLPSLQQ 670 PGPPGTEGRV EVQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cell differentiation during sexual development of the fungus Sordaria macrospora requires ATP citrate lyase activity." Nowrousian M., Masloff S., Poeggeler S., Kueck U. Mol. Cell. Biol. 19:450-460(1999) [PubMed: 9858569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC MYA-333 / DSM 997 / K(L3346) / K-hell.
[2]	"The fungal acl1 and acl2 genes encode two polypeptides with homology to the N- and C-terminal parts of the animal ATP citrate lyase polypeptide." Nowrousian M., Kueck U., Loser K., Weltring K.-M. Curr. Genet. 37:189-193(2000) [PubMed: 10794176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC MYA-333 / DSM 997 / K(L3346) / K-hell.
[3]	"De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis." Nowrousian M., Stajich J.E., Chu M., Engh I., Espagne E., Halliday K., Kamerewerd J., Kempken F., Knab B., Kuo H.-C., Osiewacz H.D., Poeggeler S., Read N.D., Seiler S., Smith K.M., Zickler D., Kueck U., Freitag M. PLoS Genet. 6:E1000891-E1000891(2010) [PubMed: 20386741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-333 / DSM 997 / K(L3346) / K-hell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224922 Genomic DNA. Translation: CAA12224.1. AJ243817 Genomic DNA. Translation: CAB76165.1. CABT02000003 Genomic DNA. Translation: CCC07573.1.
RefSeq	XP_003344998.1. XM_003344950.1.
3D structure databases
ProteinModelPortal	O93988.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10802343.
KEGG	smp:SMAC_06775.
Family and domain databases
InterPro	IPR017440. Cit_synth/succinyl-CoA_lig_AS. IPR016142. Citrate_synth-like_lrg_a-sub. IPR016143. Citrate_synth-like_sm_a-sub. IPR002020. Citrate_synthase-like. IPR016141. Citrate_synthase-like_core. IPR003781. CoA-bd. IPR005810. CoA_lig_alpha. IPR005811. CoA_ligase. IPR016040. NAD(P)-bd_dom. IPR017866. Succ-CoA_synthase_bsu_CS. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
Gene3D	G3DSA:1.10.580.10. Citrate_synthase_lrg_a-sub. 1 hit. G3DSA:1.10.230.10. Citrate_synthase_sm_a-sub. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.261. Succinyl-CoA_synth-like. 1 hit.
KO	K01648.
Pfam	PF00285. Citrate_synt. 1 hit. PF02629. CoA_binding. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PRINTS	PR01798. SCOASYNTHASE.
SUPFAM	SSF48256. Citrate_synthase_core. 1 hit.
PROSITE	PS01216. SUCCINYL_COA_LIG_1. 1 hit. PS00399. SUCCINYL_COA_LIG_2. 1 hit. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACL1_SORMK

Accession

Primary (citable) accession number: O93988
Secondary accession number(s): D1ZRT9, F7VPZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program