Reviewed, UniProtKB/Swiss-Prot O93938 (AQY2_YEAST)

Names and origin

Protein names

Recommended name:
    Aquaporin-2

Gene names

Name:	AQY2
Ordered Locus Names:	YLL052C/YLL053C
ORF Names:	L0587/L0590

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Water channel required to facilitate the transport of water across membranes. Involved in freeze tolerance, osmotolerance and cell flocculation in liquid cultures. Is nonfunctional in most laboratory strains. Ref.3 Ref.5 Ref.6 Ref.8
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Induction	During exponential phase in rich medium and repressed in minimum medium, hyper-osmolar medium or in sporulating conditions. Ref.5
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Polymorphism	In strain S288c / AB972, a natural 11 bp deletion in position 109 changes the frame and leads to a premature stop codon. This leads to a non-functional protein.
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Endoplasmic reticulum Membrane
Domain	Repeat Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-31518,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Aquaporin-2		PRO_0000227681
Regions
	Topological domain	1 – 47	47	Cytoplasmic Potential
	Transmembrane	48 – 68	21	1 Potential
	Topological domain	69 – 90	22	Extracellular Potential
	Transmembrane	91 – 111	21	2 Potential
	Topological domain	112 – 135	24	Cytoplasmic Potential
	Transmembrane	136 – 156	21	3 Potential
	Topological domain	157 – 175	19	Extracellular Potential
	Transmembrane	176 – 196	21	4 Potential
	Topological domain	197 – 202	6	Cytoplasmic Potential
	Transmembrane	203 – 223	21	5 Potential
	Topological domain	224 – 247	24	Extracellular Potential
	Transmembrane	248 – 268	21	6 Potential
	Topological domain	269 – 289	21	Cytoplasmic Potential
	Motif	117 – 119	3	NPA 1
	Motif	229 – 231	3	NPA 2
Natural variations
	Natural variant	109 – 289	181	AGVSG…QKKED → WWGFEPSRFSLFVFGQSHLT SQMCSDVVSSDHCWDGCWWC R in strain: S288c / AB972.
	Natural variant	141	1	P → S in strain: Chevalieri / ATCC 10604. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O93938-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2CA048D683B6D918	FASTA	289	31,284
	10 20 30 40 50 60 MSNESNDLEK NISHLDPTGV DNAYIPPEQP ETKHSRFNID RDTLRNHFIA AVGEFCGTFM 70 80 90 100 110 120 FLWCAYVICN VANHDVALTT EPEGSHPGQL IMIALGFGFS VMFSIWCFAG VSGGALNPAV 130 140 150 160 170 180 SLSLCLARAI SPARCVVMWF PQIIAGMAAG GAASAMTPGK VLFTNALGLG CSRSRGLFLE 190 200 210 220 230 240 MFGTAVLCLT VLMTAVEKRE TNFMAALPIG ISLFMAHMAL TGYTGTGVNP ARSLGAAVAA 250 260 270 280 RYFPHYHWIY WIGPLLGAFL AWSVWQLLQI LDYTTYVNAE KAAGQKKED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Polymorphism of Saccharomyces cerevisiae aquaporins." Laize V., Tacnet F., Ripoche P., Hohmann S. Yeast 16:897-903(2000) [PubMed: 10870101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sigma 1278B.
[2]	"Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains." Bonhivers M., Carbrey J.M., Gould S.J., Agre P. J. Biol. Chem. 273:27565-27572(1998) [PubMed: 9765289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sigma 1278B.
[3]	"Aquaporins in Saccharomyces: characterization of a second functional water channel protein." Carbrey J.M., Bonhivers M., Boeke J.D., Agre P. Proc. Natl. Acad. Sci. U.S.A. 98:1000-1005(2001) [PubMed: 11158584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-141, FUNCTION. Strain: Chevalieri / ATCC 10604 / CBS 405 / IFO 0258 / NRRL Y-1546.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Existence of a tightly regulated water channel in Saccharomyces cerevisiae." Meyrial V., Laize V., Gobin R., Ripoche P., Hohmann S., Tacnet F. Eur. J. Biochem. 268:334-343(2001) [PubMed: 11168368] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[6]	"Aquaporin expression correlates with freeze tolerance in baker's yeast, and overexpression improves freeze tolerance in industrial strains." Tanghe A., Van Dijck P., Dumortier F., Teunissen A., Hohmann S., Thevelein J.M. Appl. Environ. Microbiol. 68:5981-5989(2002) [PubMed: 12450819] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Aquaporin-mediated improvement of freeze tolerance of Saccharomyces cerevisiae is restricted to rapid freezing conditions." Tanghe A., Van Dijck P., Colavizza D., Thevelein J.M. Appl. Environ. Microbiol. 70:3377-3382(2004) [PubMed: 15184134] [Abstract] Cited for: FUNCTION.
[9]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF112860 Genomic DNA. Translation: AAD10058.1. AF056193 Genomic DNA. Translation: AAD25168.1. AF321111 Genomic DNA. Translation: AAG53971.1. Z73158 Genomic DNA. Translation: CAA97505.1. Z47973 Genomic DNA. Translation: CAA88004.1. Z73158 Genomic DNA. Translation: CAA97506.1. Z47973 Genomic DNA. Translation: CAA88005.1.
PIR	S50967. S50968.
RefSeq	NP_013047.1. NP_013048.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O93938. 2 interactions.
STRING	O93938.
Genome annotation databases
GeneID	850673. 850674.
KEGG	sce:YLL052C. sce:YLL053C.
Organism-specific databases
CYGD	YLL052c. YLL053c.
SGD	S000003975. AQY2. S000003976. AQY2.
Phylogenomic databases
HOGENOM	O93938.
OrthoDB	EOG9FR283
Gene expression databases
Genevestigator	O93938.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AQY2_YEAST

Accession

Primary (citable) accession number: O93938
Secondary accession number(s): Q12258, Q12302, Q9C411

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)