Reviewed, UniProtKB/Swiss-Prot O93934 (DHE4_BOTFU)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:

gdhA

Organism

Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)

Taxonomic identifier

40559 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Helotiales › Sclerotiniaceae › Botryotinia

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	NADP-specific glutamate dehydrogenase		PRO_0000182784
Sites
	Active site	114	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O93934-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6EDAFAB75B03C93A	FASTA	450	49,044
	10 20 30 40 50 60 MSNLPSEPEF EQAYNELAST LENSTLFEKN PEYRTALKVV SIPERVIQFR VVWEDDKNQV 70 80 90 100 110 120 QVNRGYRVQF NSALGPYKGG LRFHPTVNLS VLKFLGFEQI FKNALTGLNI GGGKGGADFD 130 140 150 160 170 180 PKGKSDNEIR RFCVSFMREL SKHIGADTDV PAGDINVGGR EIGFLFGAYK AIQNKWEGVL 190 200 210 220 230 240 TGKGGSWGGS LIRPEATGYG LVYYVAHMIQ YAGQGSFQGK RVAISGSGNV AQYAALKCIE 250 260 270 280 290 300 LGATVVSLSD SQGSLIAEGD AYFTPEDVGK IAELKLKRQS LTAFEHGGKY KYIEGSRPWT 310 320 330 340 350 360 HVKVDVALPC ATQNEVSKEE AESLVASGAR YIAEGSNMGC TQEAIDVFEA ERKEKKDKAI 370 380 390 400 410 420 WYAPGKAANA GGVAVSGLEM AQNSARISWT QEEVDEKLKD IMKNAFETGL ETPKKYVEAK 430 440 450 DGEYPSLVAG SNIAGFVKVA SAMHNHGDWW

References

[1]	"Cloning and regulation of the gdhA gene from Botrytis cinerea." Santos M. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2850.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF109684 Genomic DNA. Translation: AAC95390.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 290409.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_BOTFU

Accession

Primary (citable) accession number: O93934

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)