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O93927 (FKS1_CRYNV) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
1,3-beta-glucan synthase component FKS1
EC=2.4.1.34
Gene names
Name:FKS1
OrganismCryptococcus neoformans var. grubii (Filobasidiella neoformans var. grubii)
Taxonomic identifier178876 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length1801 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Synthesizes 1,3-beta-glucan, a major structural component of the yeast cell wall. Involved in cell wall synthesis, maintenance and cell wall remodeling By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subunit structure

Component of the 1,3-beta-glucan synthase (GS) By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Disruption phenotype

Impairs mitotic growth. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence AAD11794.1 differs from that shown. Reason: Frameshift at position 16.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 180118011,3-beta-glucan synthase component FKS1
PRO_0000394217

Regions

Transmembrane433 – 45321Helical; Potential
Transmembrane472 – 49221Helical; Potential
Transmembrane506 – 52621Helical; Potential
Transmembrane532 – 55221Helical; Potential
Transmembrane593 – 61321Helical; Potential
Transmembrane650 – 67021Helical; Potential
Transmembrane1270 – 129021Helical; Potential
Transmembrane1325 – 134521Helical; Potential
Transmembrane1424 – 144421Helical; Potential
Transmembrane1448 – 146821Helical; Potential
Transmembrane1529 – 154921Helical; Potential
Transmembrane1565 – 158521Helical; Potential
Transmembrane1607 – 162721Helical; Potential
Transmembrane1637 – 165721Helical; Potential
Transmembrane1706 – 172621Helical; Potential
Transmembrane1764 – 178421Helical; Potential
Compositional bias4 – 107Poly-Pro
Compositional bias13 – 219Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
O93927 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 6792406DD1A8BD2D

FASTA1,801204,575
        10         20         30         40         50         60 
MSYPNPPPPP KGSASFSSSS SDPFNQTNQL PYDSNAQFPP QHAFAHPSAP NPGAGGAGVA 

        70         80         90        100        110        120 
PPGQGGQYAP YYDNEPEMGG RWEGGGMGRE TWASESGWSQ NEPNYPPSDY HGGPGYLPSR 

       130        140        150        160        170        180 
ASTPTFEGSN AGHRPRDPYP AWTVDANIPL SKEEIEDVLI DLANKFGFQK DSSRNVYDFL 

       190        200        210        220        230        240 
MIQLDSRASR MSPNQALLTL HADYIGGEHA NYRKWYFAAQ LDLDDAIGAV QNPGLNRVRS 

       250        260        270        280        290        300 
VARRGGKTKN PLATAQEKSL ESATSRWRTA MNNMSQYDRL RQVALYLLCW GEAAQVRFMP 

       310        320        330        340        350        360 
ECLCFIFKCA DDYYRSPECQ NRQEAVPEGL YLRAVIKPLY RFLRDQGYEV VDGKFLRRER 

       370        380        390        400        410        420 
DHDKVIGYDD VNQLFWYPEG ISRITLNDNT RLVDIPPAQR FMKFDRIDWN KVFFKTYLEK 

       430        440        450        460        470        480 
RSFFHLLVNF NRIWVLHISV FWFFTAYNAP SIYAPSGSTT ATTPMAWSMT GLGGFVATLI 

       490        500        510        520        530        540 
MIAATLAEFS YIPTTWNNTS HLTRRLIFLL IILAITGGPS IYIAFFNQTG HVALILGIVQ 

       550        560        570        580        590        600 
FFCSVVATIA FATLPSGRMF GDRVAGKSRK YLANQTFTAS YPALGFYPRV ASFLLWFLVF 

       610        620        630        640        650        660 
GCKFTESYFF LTLSFRDPMK VMNGMKVQNC HDKYFGNGLC TNQPAFALAV MFVMDLTLFF 

       670        680        690        700        710        720 
LDTFLWYVIW NTVFSIARSF AIGMSIWTPW KDIFARLPKR IYAKILATDD MEVKYKPKVL 

       730        740        750        760        770        780 
VSQVWNAVII SMYREHLLSI EHVQKLLYHQ IQSDQPGKRT LRAPAFFISQ SEKGSKAEFF 

       790        800        810        820        830        840 
PKGSEAERRI CFFAQSLTTS IPAPIPVDAM PTFTVLVPHY SEKILLSLRE IIREEDQNTR 

       850        860        870        880        890        900 
VTLLEYLKQL HPVEWDNFVR DTKILAEESD AFNGGNPFAS DEKEEAKKAD DIPFYTIGFK 

       910        920        930        940        950        960 
SAAPEYTLRT RIWASLRAQT LYRTVSGFMN YSKAIKLLYR VENPEVVQLF GGNTDQLERE 

       970        980        990       1000       1010       1020 
LERMARRKFK FVVSMQRYSK FNKEEHENAE FLLRAYPDLQ IAYLDEEPPR KDGGESRIFS 

      1030       1040       1050       1060       1070       1080 
ALIDGHSEIM PNGRRRPKFR IELPGNPILG DGKSDNQNHA IVFYRGEYLQ LIDANQDNYL 

      1090       1100       1110       1120       1130       1140 
EECLKIRNVL GEFEEFKVST QSPYAAQGHA DFAKFPVAIL GAREYIFSEN IGILGDIAAG 

      1150       1160       1170       1180       1190       1200 
KEQTFGTLAA RSLSYIGGKL HYGHPDFLNA IYMNTRGGVS KAQKGLHLNE DIFAGMLAFG 

      1210       1220       1230       1240       1250       1260 
RGGRIKHSEY YQCGKGRDLG FGTILNFQTK IGTGMGEQML SREYYYLGTQ LPIDRFLTFY 

      1270       1280       1290       1300       1310       1320 
YGHPGFHINN ILVMMSVQVF MLALVFLGTL NKQLTVCRYS SGGDILPGQS GCYNLVPVFK 

      1330       1340       1350       1360       1370       1380 
WIKRCIISIF IVFWMAFVPL FVQELTERGT GRAILRLCKH FLSLSPVFEV FSTQIYMHSI 

      1390       1400       1410       1420       1430       1440 
LNDLTFGGAR YIATGRGFAT TRISFSILYS RFAGPSIYLG MRTLVLLLFI TLTVWVPHLI 

      1450       1460       1470       1480       1490       1500 
YFWITVVGLC VAPFLFNPHQ FAIADFIIDY REFLRWMSRG NSRTHANSWV GYCRLSRTRV 

      1510       1520       1530       1540       1550       1560 
TGFKRKRLGL PSEKLSSDVP RAPWKAILIG EIIGPICLAI LFVICYLFIK SFAVDGQIQP 

      1570       1580       1590       1600       1610       1620 
GLVRIAIIAL GPIVWNMALL ITLFLISVFL GPCLNSYTHQ FGATMAALAH FGAVAGMLVF 

      1630       1640       1650       1660       1670       1680 
FELLWFLELW NTSHAVLGII AVISVQRCIF KFLIAVFLSR EFKHDETNRA WWTGVWFNRG 

      1690       1700       1710       1720       1730       1740 
LGSHALSQPA REFVVKTIEM GLYSADFIAC HLLLALLTIP MFIPYFDRVH ATMLFWLAPN 

      1750       1760       1770       1780       1790       1800 
QQIRPPIYSF RQRSQRRKIV FKYGLLYLII QGIFIALIVV PIIFKDVAGL TPKSVPFNGI 


I

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References

[1]"A glucan synthase FKS1 homolog in cryptococcus neoformans is single copy and encodes an essential function."
Thompson J.R., Douglas C.M., Li W., Jue C.K., Pramanik B., Yuan X., Rude T.H., Toffaletti D.L., Perfect J.R., Kurtz M.
J. Bacteriol. 181:444-453(1999) [PubMed: 9882657] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
Strain: H99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF102882 Genomic DNA. Translation: AAD11794.1. Frameshift.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003440. Glyco_trans_48.
[Graphical view]
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFKS1_CRYNV
AccessionPrimary (citable) accession number: O93927
Entry history
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: January 25, 2012
This is version 36 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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