Reviewed, UniProtKB/Swiss-Prot O93883 (PGLR_PENGR)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase

Gene names

Name:

PGG1

Organism

Penicillium griseoroseum

Taxonomic identifier

84562 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 376	356	Polygalacturonase		PRO_0000024789
Sites
	Active site	215	1	Proton donor By similarity
	Active site	237	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O93883-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1EDB1EC56ED56928	FASTA	376	38,068
	10 20 30 40 50 60 MASSLKLGLI ALLGATAVNA APAAEPVLGT SLLTSRASCT FSGSSGAAEA IKSKTSCSTI 70 80 90 100 110 120 TLSNVEVPAG TTLDLTGLKS GTTVIFEGTT TFGYKEWEGP LVSVSGTSIT VQGASGAQLN 130 140 150 160 170 180 GDGARWWDGK GTNGGKTKPK FFYAHSLTNS KIENIYIKNS PVQVFSINGA KELTLSGITV 190 200 210 220 230 240 DTADGDSNGG HNTDAFDVGS SNGVYITSPI VHNQDDCLAV NSGTNVHFTG AQCTGGHGIS 250 260 270 280 290 300 IGSVGGRSDN TVDGVTVESC TIKDSDNGVR IKTVYGATGT VQGVTYKDIT LSGIAKYGIV 310 320 330 340 350 360 IEQDYENGSP TGTPTSGVPI TDLTLDNVHG TVASSGVDTY ILCASGACSD WSWSGVSITG 370 GQTSKKCKGI PSGASC

References

[1]	"Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum." Ribon A.B., Coelho J.L.C., Barros E.G., Araujo E.F. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCT 6421.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF085238 Genomic DNA. Translation: AAC83692.1.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 276795.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_PENGR

Accession

Primary (citable) accession number: O93883

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)