Reviewed, UniProtKB/Swiss-Prot O93827 (MPG1_CANAL)

Names and origin

Protein names

Recommended name:
    Mannose-1-phosphate guanyltransferase
    EC=2.7.7.13
Alternative name(s):
    ATP-mannose-1-phosphate guanylyltransferase
    GDP-mannose pyrophosphorylase
    CASRB1

Gene names

Name:	MPG1
Synonyms:	SRB1, VIG9
ORF Names:	CaO19.6190

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint By similarity.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose. Ref.2
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Mannose-1-phosphate guanyltransferase		PRO_0000068739
Natural variations
	Natural variant	197 – 198	2	ET → DP in strain: 1161.
	Natural variant	333	1	E → Q in strain: 1161.
Experimental info
	Sequence conflict	289	1	Q → R in AAC64912. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O93827-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 052DCC32D0E16923	FASTA	362	39,975
	10 20 30 40 50 60 MKGLILVGGY GTRLRPLTLT LPKPLVEFGN RPMILHQIEA LAAAGVTDIV LAVNYRPEVM 70 80 90 100 110 120 VSTLKKYEEE YGVSITFSVE EEPLGTAGPL KLAEEVLKKD DSPFFVLNSD VICDYPFKEL 130 140 150 160 170 180 ADFHKAHGAA GTIVATKVDE PSKYGVIVHD RDTPNLIDRF VEKPVEFVGN RINAGLYILN 190 200 210 220 230 240 PSVIDLIEMR PTSIEKETFP ILVEQKQLYS FDLEGYWMDV GQPKDFLSGT CLYLTSLSKK 250 260 270 280 290 300 HPEKLCKEKY VHGGNVLIDP TAKIHPSALI GPNVTIGPNV VVGEGARIQR SVLLANSQVK 310 320 330 340 350 360 DHAWVKSTIV GWNSRIGKWA RTEGVTVLGD DVEVKNEIYV NGAKVLPHKS ISSNVEKESI IM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the Candida albicans homologue of SRB1/PSA1/VIG9, the essential gene encoding GDP-mannose pyrophosphorylase in Saccharomyces cerevisiae." Warit S., Walmsley R.M., Stateva L.I. Microbiology 144:2417-2426(1998) [PubMed: 9782489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1161.
[2]	"The VIG9 gene products from the human pathogenic fungi Candida albicans and Candida glabrata encode GDP-mannose pyrophosphorylase." Ohta A., Chibana H., Arisawa M., Sudoh M. Biochim. Biophys. Acta 1475:265-272(2000) [PubMed: 10913825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY. Strain: ATCC 10259 / CBS 5796 / IFO 1060 / JCM 2078.
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF030299 Genomic DNA. Translation: AAC64911.1. AF030300 Genomic DNA. Translation: AAC64912.1. AB020596 Genomic DNA. Translation: BAA34807.1. AACQ01000225 Genomic DNA. Translation: EAK91704.1.
RefSeq	XP_710946.1.
3D structure databases
HSSP	HSSP built from PDB template 1HM0 based on UniProtKB Q97R46.
ModBase	Search...
2-D gel databases
COMPLUYEAST-2DPAGE	O93827.
Genome annotation databases
GeneID	3647454.
KEGG	cal:CaO19.6190.
Organism-specific databases
CGD	CAL0006140. SRB1.
Phylogenomic databases
OMA	LVFNADI.
Enzyme and pathway databases
BRENDA	2.7.7.13. 1124.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00132. Hexapep. 4 hits. PF00483. NTP_transferase. 1 hit. [Graphical view]
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPG1_CANAL

Accession

Primary (citable) accession number: O93827
Secondary accession number(s): Q59MI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)