O93827 (MPG1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-1-phosphate guanyltransferase
EC=2.7.7.13

Alternative name(s):
ATP-mannose-1-phosphate guanylyltransferase
CASRB1
GDP-mannose pyrophosphorylase

Gene names

Name:	MPG1
Synonyms:	SRB1, VIG9
ORF Names:	CaO19.6190

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint By similarity.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose. Ref.2
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from direct assay Ref.2. Source: CGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall mannoprotein biosynthetic process Inferred from genetic interaction Ref.1. Source: CGD
Cellular_component	cell surface Inferred from direct assay PubMed 12782322. Source: CGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 19824013. Source: CGD yeast-form cell wall Inferred from direct assay PubMed 12782322. Source: CGD
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from direct assay Ref.2. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Mannose-1-phosphate guanyltransferase		PRO_0000068739
Natural variations
	Natural variant	197 – 198	2	ET → DP in strain: 1161.
	Natural variant	333	1	E → Q in strain: 1161.
Experimental info
	Sequence conflict	289	1	Q → R in AAC64912. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O93827 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 052DCC32D0E16923	FASTA	362	39,975
	10 20 30 40 50 60 MKGLILVGGY GTRLRPLTLT LPKPLVEFGN RPMILHQIEA LAAAGVTDIV LAVNYRPEVM 70 80 90 100 110 120 VSTLKKYEEE YGVSITFSVE EEPLGTAGPL KLAEEVLKKD DSPFFVLNSD VICDYPFKEL 130 140 150 160 170 180 ADFHKAHGAA GTIVATKVDE PSKYGVIVHD RDTPNLIDRF VEKPVEFVGN RINAGLYILN 190 200 210 220 230 240 PSVIDLIEMR PTSIEKETFP ILVEQKQLYS FDLEGYWMDV GQPKDFLSGT CLYLTSLSKK 250 260 270 280 290 300 HPEKLCKEKY VHGGNVLIDP TAKIHPSALI GPNVTIGPNV VVGEGARIQR SVLLANSQVK 310 320 330 340 350 360 DHAWVKSTIV GWNSRIGKWA RTEGVTVLGD DVEVKNEIYV NGAKVLPHKS ISSNVEKESI IM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the Candida albicans homologue of SRB1/PSA1/VIG9, the essential gene encoding GDP-mannose pyrophosphorylase in Saccharomyces cerevisiae." Warit S., Walmsley R.M., Stateva L.I. Microbiology 144:2417-2426(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1161.
[2]	"The VIG9 gene products from the human pathogenic fungi Candida albicans and Candida glabrata encode GDP-mannose pyrophosphorylase." Ohta A., Chibana H., Arisawa M., Sudoh M. Biochim. Biophys. Acta 1475:265-272(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY. Strain: ATCC 10259 / CBS 5796 / DSM 5817 / JCM 2078 / NBRC 1060.
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030299 Genomic DNA. Translation: AAC64911.1. AF030300 Genomic DNA. Translation: AAC64912.1. AB020596 Genomic DNA. Translation: BAA34807.1. AACQ01000225 Genomic DNA. Translation: EAK91704.1.
RefSeq	XP_710946.1. XM_705854.1.
3D structure databases
ProteinModelPortal	O93827.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0006140.
2D gel databases
COMPLUYEAST-2DPAGE	O93827.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3647454.
KEGG	cal:CaO19.6190.
Organism-specific databases
CGD	CAL0006140. SRB1.
Phylogenomic databases
eggNOG	COG1208.
KO	K00966.
Enzyme and pathway databases
BRENDA	2.7.7.13. 1096.
UniPathway	UPA00126; UER00930.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00132. Hexapep. 2 hits. PF00483. NTP_transferase. 1 hit. [Graphical view]
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPG1_CANAL

Accession

Primary (citable) accession number: O93827
Secondary accession number(s): Q59MI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program