Reviewed, UniProtKB/Swiss-Prot O93744 (AAT_PYRKO)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase
      Short name=AspAT
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:	aspC
Ordered Locus Names:	TK0260

Organism

Pyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]

Taxonomic identifier

311400 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Aspartate aminotransferase		PRO_0000123862
Amino acid modifications
	Modified residue	200	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O93744-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2F331E69242FF499	FASTA	364	41,231
	10 20 30 40 50 60 MFNVYEFFNR INEVRPESRL DAGQPDIPVR REIIEEAVES LRRGETGYTS TGGIRELRER 70 80 90 100 110 120 IAEFEGVSAD EVIVAPGAKI LIAAEIASAK KVAVVSPRWN AYSLIARQFW REVEVIKTTL 130 140 150 160 170 180 DERWIPRVEE IKADLIIINY PNNPTGRVLS GKEIRGLLDV AEENGVKVLS DEVYAELSFT 190 200 210 220 230 240 RFTPARELYE NVVTVKGFSK LYSMTGFRLG YAIGERNEIR RIQRFIESTV TCVPPFVQRA 250 260 270 280 290 300 GVKALELRDE LIKEVRRAYL ERVRMASKML RGFDFVEPEG AFYIFLRTPQ DGMAFAERLL 310 320 330 340 350 360 SRGVAVFPGM AFGDYPNFIR ISLSGKGLER GLRVIREELE CALESRATEG WEGSSRGVSA EGSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Enzymatic characteristics of recombinant aspartate aminotransferase from Pyrococcus kodakaraensis KOD1." Jongsareejit B., Fujiwara S., Takagi M., Imanaka T. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KOD1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB012002 Genomic DNA. Translation: BAA75925.1. AP006878 Genomic DNA. Translation: BAD84449.1.
RefSeq	YP_182673.1.
3D structure databases
HSSP	HSSP built from PDB template 1O4S based on UniProtKB Q9X0Y2.
ModBase	Search...
Protein-protein interaction databases
STRING	O93744.
Genome annotation databases
GeneID	3235126.
GenomeReviews	Gene locus TK0260 in contig AP006878_GR.
KEGG	tko:TK0260.
NMPDR	fig|69014.3.peg.7.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O93744.
OMA	GLAALMW.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0260-MON.
BRENDA	2.6.1.1. 192130.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_PYRKO

Accession

Primary (citable) accession number: O93744

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)