ID   O93734_9EURY            Unreviewed;       330 AA.
AC   O93734;
DT   01-MAY-1999, integrated into UniProtKB/TrEMBL.
DT   01-MAY-1999, sequence version 1.
DT   24-JAN-2024, entry version 83.
DE   SubName: Full=F420-dependent alcohol dehydrogenase {ECO:0000313|EMBL:CAA77275.1};
DE   Flags: Fragment;
GN   Name=adf {ECO:0000313|EMBL:CAA77275.1};
OS   Methanoculleus thermophilus.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
OX   NCBI_TaxID=2200 {ECO:0000313|EMBL:CAA77275.1};
RN   [1] {ECO:0000313|EMBL:CAA77275.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Shimada M., Horigome T.;
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007829|PDB:1RHC}
RP   X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS), AND ACTIVE SITE.
RX   PubMed=15016352; DOI=10.1016/j.str.2004.02.010;
RA   Aufhammer S.W., Warkentin E., Berk H., Shima S., Thauer R.K., Ermler U.;
RT   "Coenzyme binding in F420-dependent secondary alcohol dehydrogenase, a
RT   member of the bacterial luciferase family.";
RL   Structure 12:361-370(2004).
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DR   EMBL; Y18729; CAA77275.1; -; Genomic_DNA.
DR   PDB; 1RHC; X-ray; 1.80 A; A=1-330.
DR   PDBsum; 1RHC; -.
DR   AlphaFoldDB; O93734; -.
DR   SMR; O93734; -.
DR   KEGG; ag:CAA77275; -.
DR   BRENDA; 1.1.98.5; 3272.
DR   EvolutionaryTrace; O93734; -.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   CDD; cd01097; Tetrahydromethanopterin_reductase; 1.
DR   Gene3D; 3.20.20.30; Luciferase-like domain; 1.
DR   InterPro; IPR019945; F420_G6P_DH-rel.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   NCBIfam; TIGR03557; F420_G6P_family; 1.
DR   PANTHER; PTHR43244; -; 1.
DR   PANTHER; PTHR43244:SF1; 5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE; 1.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; Bacterial luciferase-like; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:1RHC}.
FT   DOMAIN          14..304
FT                   /note="Luciferase-like"
FT                   /evidence="ECO:0000259|Pfam:PF00296"
FT   ACT_SITE        39
FT                   /note="EMO_00068 proton donor,EMO_00066 proton acceptor"
FT                   /evidence="ECO:0007829|PDB:1RHC"
FT   ACT_SITE        108
FT                   /note="EMO_00033 electrostatic stabiliser,EMO_00066 proton
FT                   acceptor,EMO_00068 proton donor"
FT                   /evidence="ECO:0007829|PDB:1RHC"
FT   SITE            43
FT                   /note="EMO_00033 electrostatic stabiliser"
FT                   /evidence="ECO:0007829|PDB:1RHC"
FT   NON_TER         330
FT                   /evidence="ECO:0000313|EMBL:CAA77275.1"
SQ   SEQUENCE   330 AA;  37158 MW;  CD1528E3B0AF11EB CRC64;
     MKTQIGYFAS LEQYRPMDAL EQAIRAEKVG FDSVWVDDHF HPWYHDNAQS AQAWAWMGAA
     LQATKKVFIS TCITCPIMRY NPAIVAQTFA TLRQMYPGRV GVAVGAGEAM NEVPVTGEWP
     SVPVRQDMTV EAVKVMRMLW ESDKPVTFKG DYFTLDKAFL YTKPDDEVPL YFSGMGPKGA
     KLAGMYGDHL MTVAAAPSTL KNVTIPKFEE GAREAGKDPS KMEHAMLIWY SVDPDYDKAV
     EALRFWAGCL VPSMFKYKVY DPKEVQLHAN LVHCDTIKEN YMCATDAEEM IKEIERFKEA
     GINHFCLGNS SPDVNFGIDI FKEVIPAVRD
//