ID   DNLI_PYRAE              Reviewed;         584 AA.
AC   O93723;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2001, sequence version 2.
DT   05-MAY-2009, entry version 60.
DE   RecName: Full=DNA ligase;
DE            EC=6.5.1.1;
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP];
GN   Name=lig; OrderedLocusNames=PAE0833;
OS   Pyrobaculum aerophilum.
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales;
OC   Thermoproteaceae; Pyrobaculum.
OX   NCBI_TaxID=13773;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827;
RA   Baikalov C.J., Slupska M.M., Miller J.H.;
RL   Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827;
RX   MEDLINE=21664397; PubMed=11792869; DOI=10.1073/pnas.241636498;
RA   Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA   Miller J.H.;
RT   "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT   aerophilum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
CC   -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA
CC       during DNA replication, DNA recombination and DNA repair (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
CC       (deoxyribonucleotide)(m) = AMP + diphosphate +
CC       (deoxyribonucleotide)(n+m).
CC   -!- COFACTOR: Divalent metal cations (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
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DR   EMBL; U82370; AAD00532.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE009789; AAL63064.1; -; Genomic_DNA.
DR   RefSeq; NP_558882.1; -.
DR   GeneID; 1465293; -.
DR   GenomeReviews; AE009441_GR; PAE0833.
DR   KEGG; pai:PAE0833; -.
DR   NMPDR; fig|178306.1.peg.572; -.
DR   HOGENOM; O93723; -.
DR   OMA; O93723; RNWLKLK.
DR   BioCyc; PAER178306:PAE0833-MON; -.
DR   BRENDA; 6.5.1.1; 142830.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:HAMAP.
DR   GO; GO:0006281; P:DNA repair; IEA:HAMAP.
DR   GO; GO:0006260; P:DNA replication; IEA:HAMAP.
DR   HAMAP; MF_00407; -; 1.
DR   InterPro; IPR000977; DNA_ligase.
DR   InterPro; IPR012309; DNA_ligase_A_C.
DR   InterPro; IPR012310; DNA_ligase_A_M.
DR   InterPro; IPR012308; DNA_ligase_A_N.
DR   InterPro; IPR016059; DNA_ligase_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; FALSE_NEG.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage;
KW   DNA recombination; DNA repair; DNA replication; Ligase; Metal-binding;
KW   Nucleotide-binding.
FT   CHAIN         1    584       DNA ligase.
FT                                /FTId=PRO_0000059611.
FT   ACT_SITE    251    251       N6-AMP-lysine intermediate (By
FT                                similarity).
FT   BINDING     249    249       ATP (By similarity).
FT   BINDING     256    256       ATP (By similarity).
FT   BINDING     271    271       ATP (By similarity).
FT   BINDING     301    301       ATP (By similarity).
FT   BINDING     341    341       ATP (By similarity).
FT   BINDING     416    416       ATP (By similarity).
FT   BINDING     422    422       ATP (By similarity).
SQ   SEQUENCE   584 AA;  64568 MW;  7CB44E8A5B0DBA0A CRC64;
     MQFGELVKAL AAIEATTQRT TMVKLLVSLF KKASPEEVGK IVYFILGDLR PPWEGVELGV
     AEKLCLRAVS KATGAAVSEL EALYKKTGDV GEAARRALAA SKRPGLLAFG SQKPLEVSEV
     YDTLLKVARA TGEGAQDMKV ALLSSLFARA TPEEGKYIAR FVVGKLRLGV ADMTIIEALS
     DAYGVNKEAL EKAYHIYPDL GRLAKHVAEG KPLDEIRITP GVPVLPMLAQ RLSSASEILA
     KLGGSAICEY KYDGERAQIH LTPGGVKIFS RRLEDITHAY PDVVKAVKEA VSAKEAILEG
     EIVAVDPDTG DMLPFQELMH RKRKHDVATA VELYPTVLYL FDVLYVDGED LTEEPLIYRR
     VRLSEIVCET DKVSIAKWRI FDNAEAVDVF FHESVSMGTE GLICKSPSSI YEMGARGWNW
     IKYKRDYRSE MIDTVDLVVV GAFHGRGKRA GLYGAFLLAA YDPSTDMFYT VCKVGSGFTD
     ADLKKMYEIL QPFKIPHRHP RVVSKMEADV WFVPQVVIEV IGAEITLSPL HTCCLGAVRP
     GVGLAVRFPR FTGRYRSDKS PEQATTVAEM LELYKRQKKV VQPE
//