Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O93723

- DNLI_PYRAE

UniProt

O93723 - DNLI_PYRAE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA ligase

Gene

lig

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Divalent metal cations.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei249 – 2491ATPBy similarity
Active sitei251 – 2511N6-AMP-lysine intermediateBy similarity
Binding sitei256 – 2561ATPBy similarity
Binding sitei271 – 2711ATPBy similarity
Binding sitei301 – 3011ATPBy similarity
Binding sitei341 – 3411ATPBy similarity
Binding sitei416 – 4161ATPBy similarity
Binding sitei422 – 4221ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER178306:GCEO-586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase (EC:6.5.1.1)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene namesi
Name:lig
Ordered Locus Names:PAE0833
OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Taxonomic identifieri178306 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
ProteomesiUP000002439: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584DNA ligasePRO_0000059611Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi178306.PAE0833.

Structurei

3D structure databases

ProteinModelPortaliO93723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
InParanoidiO93723.
KOiK10747.
OMAiCTDIGEL.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O93723-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFGELVKAL AAIEATTQRT TMVKLLVSLF KKASPEEVGK IVYFILGDLR
60 70 80 90 100
PPWEGVELGV AEKLCLRAVS KATGAAVSEL EALYKKTGDV GEAARRALAA
110 120 130 140 150
SKRPGLLAFG SQKPLEVSEV YDTLLKVARA TGEGAQDMKV ALLSSLFARA
160 170 180 190 200
TPEEGKYIAR FVVGKLRLGV ADMTIIEALS DAYGVNKEAL EKAYHIYPDL
210 220 230 240 250
GRLAKHVAEG KPLDEIRITP GVPVLPMLAQ RLSSASEILA KLGGSAICEY
260 270 280 290 300
KYDGERAQIH LTPGGVKIFS RRLEDITHAY PDVVKAVKEA VSAKEAILEG
310 320 330 340 350
EIVAVDPDTG DMLPFQELMH RKRKHDVATA VELYPTVLYL FDVLYVDGED
360 370 380 390 400
LTEEPLIYRR VRLSEIVCET DKVSIAKWRI FDNAEAVDVF FHESVSMGTE
410 420 430 440 450
GLICKSPSSI YEMGARGWNW IKYKRDYRSE MIDTVDLVVV GAFHGRGKRA
460 470 480 490 500
GLYGAFLLAA YDPSTDMFYT VCKVGSGFTD ADLKKMYEIL QPFKIPHRHP
510 520 530 540 550
RVVSKMEADV WFVPQVVIEV IGAEITLSPL HTCCLGAVRP GVGLAVRFPR
560 570 580
FTGRYRSDKS PEQATTVAEM LELYKRQKKV VQPE
Length:584
Mass (Da):64,568
Last modified:June 20, 2001 - v2
Checksum:i7CB44E8A5B0DBA0A
GO

Sequence cautioni

The sequence AAD00532.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82370 Genomic DNA. Translation: AAD00532.1. Different initiation.
AE009441 Genomic DNA. Translation: AAL63064.1.
RefSeqiNP_558882.1. NC_003364.1.
WP_011007536.1. NC_003364.1.

Genome annotation databases

EnsemblBacteriaiAAL63064; AAL63064; PAE0833.
GeneIDi1465293.
KEGGipai:PAE0833.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82370 Genomic DNA. Translation: AAD00532.1 . Different initiation.
AE009441 Genomic DNA. Translation: AAL63064.1 .
RefSeqi NP_558882.1. NC_003364.1.
WP_011007536.1. NC_003364.1.

3D structure databases

ProteinModelPortali O93723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 178306.PAE0833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL63064 ; AAL63064 ; PAE0833 .
GeneIDi 1465293.
KEGGi pai:PAE0833.

Phylogenomic databases

eggNOGi COG1793.
HOGENOMi HOG000036008.
InParanoidi O93723.
KOi K10747.
OMAi CTDIGEL.

Enzyme and pathway databases

BioCyci PAER178306:GCEO-586-MONOMER.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPi MF_00407. DNA_ligase.
InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Baikalov C.J., Slupska M.M., Miller J.H.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
  2. "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
    Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
    Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Entry informationi

Entry nameiDNLI_PYRAE
AccessioniPrimary (citable) accession number: O93723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3