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O93723 (DNLI_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:PAE0833
OrganismPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827) [Reference proteome] [HAMAP]
Taxonomic identifier178306 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence caution

The sequence AAD00532.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 584584DNA ligase HAMAP-Rule MF_00407
PRO_0000059611

Sites

Active site2511N6-AMP-lysine intermediate By similarity
Binding site2491ATP By similarity
Binding site2561ATP By similarity
Binding site2711ATP By similarity
Binding site3011ATP By similarity
Binding site3411ATP By similarity
Binding site4161ATP By similarity
Binding site4221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O93723 [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: 7CB44E8A5B0DBA0A

FASTA58464,568
        10         20         30         40         50         60 
MQFGELVKAL AAIEATTQRT TMVKLLVSLF KKASPEEVGK IVYFILGDLR PPWEGVELGV 

        70         80         90        100        110        120 
AEKLCLRAVS KATGAAVSEL EALYKKTGDV GEAARRALAA SKRPGLLAFG SQKPLEVSEV 

       130        140        150        160        170        180 
YDTLLKVARA TGEGAQDMKV ALLSSLFARA TPEEGKYIAR FVVGKLRLGV ADMTIIEALS 

       190        200        210        220        230        240 
DAYGVNKEAL EKAYHIYPDL GRLAKHVAEG KPLDEIRITP GVPVLPMLAQ RLSSASEILA 

       250        260        270        280        290        300 
KLGGSAICEY KYDGERAQIH LTPGGVKIFS RRLEDITHAY PDVVKAVKEA VSAKEAILEG 

       310        320        330        340        350        360 
EIVAVDPDTG DMLPFQELMH RKRKHDVATA VELYPTVLYL FDVLYVDGED LTEEPLIYRR 

       370        380        390        400        410        420 
VRLSEIVCET DKVSIAKWRI FDNAEAVDVF FHESVSMGTE GLICKSPSSI YEMGARGWNW 

       430        440        450        460        470        480 
IKYKRDYRSE MIDTVDLVVV GAFHGRGKRA GLYGAFLLAA YDPSTDMFYT VCKVGSGFTD 

       490        500        510        520        530        540 
ADLKKMYEIL QPFKIPHRHP RVVSKMEADV WFVPQVVIEV IGAEITLSPL HTCCLGAVRP 

       550        560        570        580 
GVGLAVRFPR FTGRYRSDKS PEQATTVAEM LELYKRQKKV VQPE 

« Hide

References

« Hide 'large scale' references
[1]Baikalov C.J., Slupska M.M., Miller J.H.
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
[2]"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U82370 Genomic DNA. Translation: AAD00532.1. Different initiation.
AE009441 Genomic DNA. Translation: AAL63064.1.
RefSeqNP_558882.1. NC_003364.1.

3D structure databases

ProteinModelPortalO93723.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING178306.PAE0833.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL63064; AAL63064; PAE0833.
GeneID1465293.
KEGGpai:PAE0833.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAYIARFVV.
ProtClustDBPRK01109.

Enzyme and pathway databases

BioCycPAER178306:GCEO-586-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_PYRAE
AccessionPrimary (citable) accession number: O93723
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: February 19, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families