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O93723

- DNLI_PYRAE

UniProt

O93723 - DNLI_PYRAE

Protein

DNA ligase

Gene

lig

Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (20 Jun 2001)
      Previous versions | rss
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    Functioni

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

    Cofactori

    Divalent metal cations.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei249 – 2491ATPBy similarity
    Active sitei251 – 2511N6-AMP-lysine intermediateBy similarity
    Binding sitei256 – 2561ATPBy similarity
    Binding sitei271 – 2711ATPBy similarity
    Binding sitei301 – 3011ATPBy similarity
    Binding sitei341 – 3411ATPBy similarity
    Binding sitei416 – 4161ATPBy similarity
    Binding sitei422 – 4221ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation involved in DNA repair Source: InterPro
    4. DNA recombination Source: UniProtKB-HAMAP
    5. DNA replication Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPAER178306:GCEO-586-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase (EC:6.5.1.1)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
    Gene namesi
    Name:lig
    Ordered Locus Names:PAE0833
    OrganismiPyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
    Taxonomic identifieri178306 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
    ProteomesiUP000002439: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584DNA ligasePRO_0000059611Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi178306.PAE0833.

    Structurei

    3D structure databases

    ProteinModelPortaliO93723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000036008.
    KOiK10747.
    OMAiCTDIGEL.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPiMF_00407. DNA_ligase.
    InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O93723-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQFGELVKAL AAIEATTQRT TMVKLLVSLF KKASPEEVGK IVYFILGDLR    50
    PPWEGVELGV AEKLCLRAVS KATGAAVSEL EALYKKTGDV GEAARRALAA 100
    SKRPGLLAFG SQKPLEVSEV YDTLLKVARA TGEGAQDMKV ALLSSLFARA 150
    TPEEGKYIAR FVVGKLRLGV ADMTIIEALS DAYGVNKEAL EKAYHIYPDL 200
    GRLAKHVAEG KPLDEIRITP GVPVLPMLAQ RLSSASEILA KLGGSAICEY 250
    KYDGERAQIH LTPGGVKIFS RRLEDITHAY PDVVKAVKEA VSAKEAILEG 300
    EIVAVDPDTG DMLPFQELMH RKRKHDVATA VELYPTVLYL FDVLYVDGED 350
    LTEEPLIYRR VRLSEIVCET DKVSIAKWRI FDNAEAVDVF FHESVSMGTE 400
    GLICKSPSSI YEMGARGWNW IKYKRDYRSE MIDTVDLVVV GAFHGRGKRA 450
    GLYGAFLLAA YDPSTDMFYT VCKVGSGFTD ADLKKMYEIL QPFKIPHRHP 500
    RVVSKMEADV WFVPQVVIEV IGAEITLSPL HTCCLGAVRP GVGLAVRFPR 550
    FTGRYRSDKS PEQATTVAEM LELYKRQKKV VQPE 584
    Length:584
    Mass (Da):64,568
    Last modified:June 20, 2001 - v2
    Checksum:i7CB44E8A5B0DBA0A
    GO

    Sequence cautioni

    The sequence AAD00532.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82370 Genomic DNA. Translation: AAD00532.1. Different initiation.
    AE009441 Genomic DNA. Translation: AAL63064.1.
    RefSeqiNP_558882.1. NC_003364.1.

    Genome annotation databases

    EnsemblBacteriaiAAL63064; AAL63064; PAE0833.
    GeneIDi1465293.
    KEGGipai:PAE0833.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82370 Genomic DNA. Translation: AAD00532.1 . Different initiation.
    AE009441 Genomic DNA. Translation: AAL63064.1 .
    RefSeqi NP_558882.1. NC_003364.1.

    3D structure databases

    ProteinModelPortali O93723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 178306.PAE0833.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL63064 ; AAL63064 ; PAE0833 .
    GeneIDi 1465293.
    KEGGi pai:PAE0833.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000036008.
    KOi K10747.
    OMAi CTDIGEL.

    Enzyme and pathway databases

    BioCyci PAER178306:GCEO-586-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPi MF_00407. DNA_ligase.
    InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Baikalov C.J., Slupska M.M., Miller J.H.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
    2. "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum."
      Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H.
      Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.

    Entry informationi

    Entry nameiDNLI_PYRAE
    AccessioniPrimary (citable) accession number: O93723
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: June 20, 2001
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3