Reviewed, UniProtKB/Swiss-Prot O93544 (LDHA_DISMA)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27

Gene names

Name:

ldha

Organism

Dissostichus mawsoni (Antarctic cod)

Taxonomic identifier

36200 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Perciformes › Notothenioidei › Nototheniidae › Dissostichus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 331	330	L-lactate dehydrogenase A chain		PRO_0000168436
Regions
	Nucleotide binding	29 – 57	29	NAD By similarity
Sites
	Active site	192	1	Proton acceptor By similarity
	Binding site	98	1	NAD By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	137	1	NAD or substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O93544-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3AA68DF03766FE65	FASTA	331	36,142
	10 20 30 40 50 60 MSTKEKLISH VMKEEPVGSR NKVTVVGVGM VGMASAISIL LKDLCDELAM VDVMEDKLKG 70 80 90 100 110 120 EVMDLQHGSL FLKTKIVGDK DYSVTANSKV VVVTAGARQQ EGESRLNLVQ RNVNIFKFII 130 140 150 160 170 180 PNIVKYSPNC ILMVVSNPVD ILTYVAWKLS GFPRNRVIGS GTNLDSARFR HLIGEKLHLH 190 200 210 220 230 240 PSSCHAWIVG EHGDSSVPVW SGVNVAGVSL QGLNPQMGTE GDGENWKAIH KEVVDGAYEV 250 260 270 280 290 300 IKLKGYTSWA IGMSVADLVE SIIKNMHKVH PVSTLVQGMH GVKDEVFLSV PSVLGNSGLT 310 320 330 DVIHMTLKAE EEKQLQKSAE TLWGVQKELV L

References

[1]

"Hot spots in cold adaptation: localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes." Fields P.A., Somero G.N. Proc. Natl. Acad. Sci. U.S.A. 95:11476-11481(1998) [PubMed: 9736762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF079827 mRNA. Translation: AAC63285.1.
3D structure databases
HSSP	HSSP built from PDB template 9LDT based on UniProtKB P00339.
SMR	O93544. Positions 2-331.
ModBase	Search...
Phylogenomic databases
HOVERGEN	O93544.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHA_DISMA

Accession

Primary (citable) accession number: O93544

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)