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Protein

L-lactate dehydrogenase A chain

Gene

ldha

Organism
Champsocephalus gunnari (Mackerel icefish)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase A chain (ldha)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981NADBy similarity
Binding sitei105 – 1051SubstrateBy similarity
Binding sitei137 – 1371NAD or substrateBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Active sitei192 – 1921Proton acceptorBy similarity
Binding sitei247 – 2471SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 5729NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.27. 9919.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Gene namesi
Name:ldha
OrganismiChampsocephalus gunnari (Mackerel icefish)
Taxonomic identifieri52237 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataEupercariaPerciformesNotothenioideiChannichthyidaeChampsocephalus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 331330L-lactate dehydrogenase A chainPRO_0000168431Add
BLAST

Proteomic databases

PRIDEiO93541.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Secondary structure

1
331
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 75Combined sources
Beta strandi23 – 264Combined sources
Helixi30 – 4112Combined sources
Beta strandi46 – 516Combined sources
Helixi55 – 6612Combined sources
Helixi67 – 715Combined sources
Beta strandi74 – 785Combined sources
Helixi82 – 854Combined sources
Beta strandi89 – 935Combined sources
Helixi105 – 12622Combined sources
Beta strandi131 – 1344Combined sources
Beta strandi136 – 1383Combined sources
Helixi139 – 14810Combined sources
Helixi154 – 1563Combined sources
Beta strandi157 – 1593Combined sources
Helixi163 – 17715Combined sources
Helixi181 – 1833Combined sources
Beta strandi188 – 1936Combined sources
Helixi200 – 2023Combined sources
Helixi210 – 2134Combined sources
Turni215 – 2184Combined sources
Helixi226 – 2349Combined sources
Helixi236 – 2449Combined sources
Helixi249 – 26315Combined sources
Beta strandi268 – 2758Combined sources
Beta strandi287 – 2959Combined sources
Beta strandi298 – 3025Combined sources
Helixi309 – 32719Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V65X-ray2.35A/B2-331[»]
ProteinModelPortaliO93541.
SMRiO93541. Positions 2-331.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO93541.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOVERGENiHBG000462.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O93541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKEKLISH VMKEEPVGSR SKVTVVGVGM VGMASAISIL LKDLCDELAM
60 70 80 90 100
VDVMEDKLKG EVMDLQHGSL FLKTKIVGDK DYSVTANSKV VVVTAGARQQ
110 120 130 140 150
EGESRLNLVQ RNVNIFKFII PNIVKYSPNC ILMVVSNPVD ILTYVAWKLS
160 170 180 190 200
GFPRHRVIGS GTNLDSARFR HLIGEKLHLH PSSCHAWIVG EHGDSSVPVW
210 220 230 240 250
SGVNVAGVSL QGLNPQMGTE GDGENWKAIH KEVVDGAYEV IKLKGYTSWA
260 270 280 290 300
IGMSVADLVE SIIKNMHKVH PVSTLVQGMH GVKDEVFLSV PCVLGNSGLT
310 320 330
DVIHMTLKAE EEKQLQKSAE TLWGVQKELT L
Length:331
Mass (Da):36,156
Last modified:January 23, 2007 - v3
Checksum:i556546DC3A03237E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079824 mRNA. Translation: AAC63282.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079824 mRNA. Translation: AAC63282.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V65X-ray2.35A/B2-331[»]
ProteinModelPortaliO93541.
SMRiO93541. Positions 2-331.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO93541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG000462.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BRENDAi1.1.1.27. 9919.

Miscellaneous databases

EvolutionaryTraceiO93541.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_CHAGU
AccessioniPrimary (citable) accession number: O93541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 23, 2007
Last modified: December 9, 2015
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.