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Protein

Werner syndrome ATP-dependent helicase homolog

Gene

wrn

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).By similarity2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarity, Zn2+By similarityNote: Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Magnesium 1; catalyticBy similarity
Metal bindingi76 – 761Magnesium 2; catalyticBy similarity
Metal bindingi78 – 781Magnesium 1; catalyticBy similarity
Metal bindingi210 – 2101Magnesium 1; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi512 – 5198ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Werner syndrome ATP-dependent helicase homolog (EC:3.6.4.12By similarity)
Alternative name(s):
Exonuclease WRN (EC:3.1.-.-)
Focus-forming activity 1
Short name:
FFA-1
Gene namesi
Name:wrn
Synonyms:ffa1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-992879. wrn.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14361436Werner syndrome ATP-dependent helicase homologPRO_0000205047Add
BLAST

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei139 – 1391Interaction with DNABy similarity
Sitei980 – 9801Interaction with DNABy similarity

Structurei

3D structure databases

ProteinModelPortaliO93530.
SMRiO93530. Positions 32-225, 892-1017, 1090-1182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 2231733'-5' exonucleaseAdd
BLAST
Domaini499 – 665167Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini690 – 842153Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini1098 – 117780HRDCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni930 – 9367Interaction with DNABy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi609 – 6124DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi461 – 4677Poly-Asp

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated
Contains 1 3'-5' exonuclease domain.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HRDC domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG000325.
KOiK10900.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.30.420.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR029491. Helicase_HTH.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR012337. RNaseH-like_dom.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF01612. DNA_pol_A_exo1. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF14493. HTH_40. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00474. 35EXOc. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O93530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLQRKLPE WMSVKQQEDR IDDAKKSFCK KNILEDNLPF MKFNGSIVYS
60 70 80 90 100
YESNDCSLLS EDIRSSLLEE DVLGFDIEWP PVYTKGKTGK VALIQVCVSE
110 120 130 140 150
KKCYLFHISP MAGFPKGLKR LLEDESVRKV GVGIEGDQWK LMSDYELKLK
160 170 180 190 200
GFIELSEMAN QKLRCKEKWT FNGLIKHLFK EQLYKRKSYR CSNWDIFLLT
210 220 230 240 250
EDQKLYAATD AYAGLLIYKK LEGMDAHESD SFRVGREGVA DCKGVKRQLT
260 270 280 290 300
DLSKGLMDLV NQVPNSFGCY TEAVRAVDIL EDLSEKLEEL RNIMKEASKA
310 320 330 340 350
EGNGLHFQNS EDCSKKDKSI LHVACKESLA EHKMDCKNAD SQNNKDIDSC
360 370 380 390 400
QNENRDEDFF MTLGISEEEL YMMEREDDKK QTNPDYKLNK DSCDTNEEKD
410 420 430 440 450
MSYVIESDED FDSEIIKSLE DLDNSTEEAL GTGVPQAGLI PAKSVDTVAD
460 470 480 490 500
EEEDEGIEEE DDDDDWDPSM PEPSAQHISC LKTYFGHSSF KPVQWKVVHS
510 520 530 540 550
VLRERRDNLV VMATGYGKSL CYQFAPVYTS GIGIVICPLI SLMEDQVLQL
560 570 580 590 600
EMSNISSCFL GSAQSKNVLQ DVKDGKMRVI YMTPEFCSRG ISLLQDLDNR
610 620 630 640 650
YGITLIAIDE AHCISEWGHD FRSAYRSLGS LKRMLPNVPI VALTATASPS
660 670 680 690 700
IREDITKSLN LHNPQVTCTS FDRPNLYLDV ARKTTNISID LRQFLIKKQQ
710 720 730 740 750
GSGWEFEGAT IVYCPTRKTS EQVTAELIKL GIACGTYHAG MGIKQRREVH
760 770 780 790 800
HRFMRDEIHC VVATVAFGMG INKPDIRKVI HYGAPKEMES YYQEIGRAGR
810 820 830 840 850
DGLPSCCHAL WAQADMNFNR HMLGEIPNKG FREYKLKMLT KMEKYLNSST
860 870 880 890 900
CRRKIILSHF EDKQLRKASS GIMGTEKCCD NCKTRLICNI SINDTEDNLQ
910 920 930 940 950
DFGPQAYKFI SAVDVLGQKF GTGVPVLFLR GSTSQRVPDR FRNHSLFSSG
960 970 980 990 1000
KDQTEAFWKV LARQLITEGY LQESSGQTKF STICGLTSKG SNWLIKANNE
1010 1020 1030 1040 1050
QCPSLLLPSN NELCLQRTRV SNFSSAQAHS SMVPHASSNT RSSMPKAGPE
1060 1070 1080 1090 1100
KMELKDKFSY QEAERLSKAA GVSKSSFKLQ TPCKLSRPPE PEVSPREREL
1110 1120 1130 1140 1150
QTTLYGRLVV ARQKIASERD ILPAVLATNK VLVDMAKLRP TTSENMKKLD
1160 1170 1180 1190 1200
GVSEAKSAML APLLEVVKEF CIANSLKVDV FSGSVSQSES TFFTPREQER
1210 1220 1230 1240 1250
ISLPESQRMS YSLFQEQNLS LKKIADVRCL SMAVVGMHLW QALKAGYSFD
1260 1270 1280 1290 1300
VQRAGLTPEM KKLITYAIKK PPINSDLSSF KAIREYVPAN IDGYPIRMVI
1310 1320 1330 1340 1350
SLLEKEGSSG AQGQPEFPTQ KTLIQTEENP KNVSVQNTKH KVTMGKSMWI
1360 1370 1380 1390 1400
EKKPTQPATA ELEVTKGKAL APIMLASWNE ASLDADTEEL FSESQSSTTR
1410 1420 1430
PRRRLPEWFG STKGNAATRC IQESKNLGEE KGSFFD
Length:1,436
Mass (Da):161,851
Last modified:November 1, 1998 - v1
Checksum:i1BEAF05A25B4E230
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067418 mRNA. Translation: AAC63512.1.
PIRiT14895.
RefSeqiNP_001081838.1. NM_001088369.1.
UniGeneiXl.404.

Genome annotation databases

GeneIDi398079.
KEGGixla:398079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067418 mRNA. Translation: AAC63512.1.
PIRiT14895.
RefSeqiNP_001081838.1. NM_001088369.1.
UniGeneiXl.404.

3D structure databases

ProteinModelPortaliO93530.
SMRiO93530. Positions 32-225, 892-1017, 1090-1182.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398079.
KEGGixla:398079.

Organism-specific databases

CTDi7486.
XenbaseiXB-GENE-992879. wrn.

Phylogenomic databases

HOVERGENiHBG000325.
KOiK10900.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.30.420.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR029491. Helicase_HTH.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR012337. RNaseH-like_dom.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF01612. DNA_pol_A_exo1. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF14493. HTH_40. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00474. 35EXOc. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Replication focus-forming activity 1 and the Werner syndrome gene product."
    Yan H., Chen C.-Y., Kobayashi R., Newport J.
    Nat. Genet. 19:375-378(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "FFA-1, a protein that promotes the formation of replication centers within nuclei."
    Yan H., Newport J.
    Science 269:1883-1885(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiWRN_XENLA
AccessioniPrimary (citable) accession number: O93530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: November 1, 1998
Last modified: January 20, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.