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Protein

Zinc metalloproteinase-disintegrin-like bothropasin

Gene
N/A
Organism
Bothrops jararaca (Jararaca) (Bothrops jajaraca)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Zinc metalloproteinase-disintegrin-like bothropasin: has caseinolytic activity. Causes hemorrhage on rabbit skin and causes myonecrosis in mouse tibialis anterior muscle.
Disintegrin-like bothropasin: inhibits platelet aggregation.

Catalytic activityi

Cleavage of 5-His-|-Leu-6, 10-His-|-Leu-11, 14-Ala-|-Leu-15, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 in insulin B chain.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by EDTA and EGTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi201Calcium 11
Metal bindingi285Calcium 11
Metal bindingi334Zinc; catalytic1 Publication1
Active sitei335PROSITE-ProRule annotation1
Metal bindingi338Zinc; catalytic1 Publication1
Metal bindingi344Zinc; catalytic1 Publication1
Sitei378Necessary, but not sufficient, for proteolytic processingBy similarity1
Metal bindingi389Calcium 1; via carbonyl oxygen1
Metal bindingi392Calcium 11
Metal bindingi404Calcium 2; via carbonyl oxygen1
Metal bindingi407Calcium 21
Metal bindingi409Calcium 2; via carbonyl oxygen1
Metal bindingi411Calcium 21
Metal bindingi414Calcium 21
Metal bindingi417Calcium 21
Metal bindingi468Calcium 31
Metal bindingi469Calcium 3; via carbonyl oxygen1
Metal bindingi471Calcium 31
Metal bindingi483Calcium 31
Metal bindingi484Calcium 3; via carbonyl oxygen1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Cell adhesion impairing toxin, Hemorrhagic toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.49. 911.

Protein family/group databases

MEROPSiM12.140.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase-disintegrin-like bothropasin (EC:3.4.24.49)
Alternative name(s):
Snake venom metalloproteinase
Short name:
SVMP
Cleaved into the following chain:
OrganismiBothrops jararaca (Jararaca) (Bothrops jajaraca)
Taxonomic identifieri8724 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000032641821 – 191By similarityAdd BLAST171
ChainiPRO_0000326419192 – 610Zinc metalloproteinase-disintegrin-like bothropasinAdd BLAST419
ChainiPRO_0000326420399 – 610Disintegrin-like bothropasinBy similarityAdd BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi309 ↔ 389In zinc metalloproteinase-disintegrin-like bothropasin1 Publication
Disulfide bondi349 ↔ 373In zinc metalloproteinase-disintegrin-like bothropasin1 Publication
Disulfide bondi351 ↔ 356In zinc metalloproteinase-disintegrin-like bothropasin1 Publication
Glycosylationi372N-linked (GlcNAc...)1 Publication1
Disulfide bondi405 ↔ 434In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi405 ↔ 424In disintegrin-like bothropasin; alternateBy similarity
Disulfide bondi416 ↔ 434In disintegrin-like bothropasin; alternateBy similarity
Disulfide bondi416 ↔ 429In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi418 ↔ 424In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi428 ↔ 451In zinc metalloproteinase-disintegrin-like bothropasin1 Publication
Disulfide bondi442 ↔ 448In zinc metalloproteinase-disintegrin-like bothropasin1 Publication
Disulfide bondi447 ↔ 473In zinc metalloproteinase-disintegrin-like bothropasin1 Publication
Disulfide bondi460 ↔ 480In both disintegrin-like bothropasin and zinc metalloproteinase-disintegrin-like bothropasinPROSITE-ProRule annotation1 Publication
Disulfide bondi467 ↔ 499In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi467 ↔ 492In disintegrin-like bothropasin; alternateBy similarity
Disulfide bondi492 ↔ 504In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi499 ↔ 504In disintegrin-like bothropasinBy similarity
Disulfide bondi511 ↔ 561In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi511 ↔ 526In disintegrin-like bothropasin; alternateBy similarity
Disulfide bondi526 ↔ 572In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi539 ↔ 549In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi549 ↔ 556In disintegrin-like bothropasin; alternateBy similarity
Disulfide bondi556 ↔ 598In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi561 ↔ 572In disintegrin-like bothropasinBy similarity
Disulfide bondi592 ↔ 603In zinc metalloproteinase-disintegrin-like bothropasin; alternate1 Publication
Disulfide bondi598 ↔ 603In disintegrin-like bothropasinBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.2 Publications

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DSLX-ray2.70A/B192-610[»]
ProteinModelPortaliO93523.
SMRiO93523.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO93523.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 394Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
Domaini402 – 488DisintegrinPROSITE-ProRule annotationAdd BLAST87

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi466 – 468D/ECD-tripeptide3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi489 – 610Cys-richAdd BLAST122

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.
KOiK20745.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O93523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEVLLVTIC LAAFPYQGSS IILESGNVND YEVVYPRKVT ALPKGAVQPK
60 70 80 90 100
YEDAMQYEFK VNGEPVVLHL EKNKGLFSKD YSETHYSPDG REITTYPAVE
110 120 130 140 150
DHCYYHGRIE NDADSTASIS ACNGLKGHFK LQRETYFIEP LKLSNSEAHA
160 170 180 190 200
VFKYENVEKE DEAPKMCGVT QNWKSYEPIK KASQLVVTAE QQKYNPFRYV
210 220 230 240 250
ELFIVVDQGM VTKNNGDLDK IKARMYELAN IVNEILRYLY MHAALVGLEI
260 270 280 290 300
WSNGDKITVK PDVDYTLNSF AEWRKTDLLT RKKHDNAQLL TAIDFNGPTI
310 320 330 340 350
GYAYIGSMCH PKRSVAIVED YSPINLVVAV IMAHEMGHNL GIHHDTDFCS
360 370 380 390 400
CGDYPCIMGP TISNEPSKFF SNCSYIQCWD FIMKENPQCI LNEPLGTDIV
410 420 430 440 450
SPPVCGNELL EVGEECDCGT PENCQNECCD AATCKLKSGS QCGHGDCCEQ
460 470 480 490 500
CKFSKSGTEC RASMSECDPA EHCTGQSSEC PADVFHKNGQ PCLDNYGYCY
510 520 530 540 550
NGNCPIMYHQ CYALFGADVY EAEDSCFKDN QKGNYYGYCR KENGKKIPCA
560 570 580 590 600
PEDVKCGRLY CKDNSPGQNN PCKMFYSNDD EHKGMVLPGT KCADGKVCSN
610
GHCVDVATAY
Length:610
Mass (Da):68,213
Last modified:November 1, 1999 - v2
Checksum:i014C8AE6B86F25DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056025 mRNA. Translation: AAC61986.2.

Genome annotation databases

KEGGiag:AAC61986.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056025 mRNA. Translation: AAC61986.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DSLX-ray2.70A/B192-610[»]
ProteinModelPortaliO93523.
SMRiO93523.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM12.140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC61986.

Phylogenomic databases

HOVERGENiHBG006978.
KOiK20745.

Enzyme and pathway databases

BRENDAi3.4.24.49. 911.

Miscellaneous databases

EvolutionaryTraceiO93523.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVM3BP_BOTJA
AccessioniPrimary (citable) accession number: O93523
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The metalloproteinase domain which is released from the cleavage of the disintegrin bothropasin may be unstable.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.