Reviewed, UniProtKB/Swiss-Prot O93456 (DEM_PACDA)

Names and origin

Protein names	Recommended name:     Dermorphin Cleaved into the following 2 chains:     1- Recommended name:             [Ile2]-deltorphin     2- Recommended name:             Dermorphin
Organism	Pachymedusa dacnicolor (Giant mexican leaf frog)
Taxonomic identifier	75988 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Hyloidea › Hylidae › Phyllomedusinae › Pachymedusa

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Dermorphin has a very potent opiate-like activity. It has high affinity and selectivity for mu-type opioid receptors By similarity. UniProtKB P05422 Deltorphin has a very potent opiate-like activity. It has high affinity and selectivity for delta-type opioid receptors By similarity. UniProtKB P05422
Subcellular location	Secreted.
Tissue specificity	Expressed by the skin glands.
Sequence similarities	Belongs to the frog skin active peptide (FSAP) family. Dermorphin subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Amphibian defense peptide Endorphin Opioid peptide
PTM	Amidation Cleavage on pair of basic residues D-amino acid
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: InterPro neuropeptide signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	opioid peptide activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 46	24			PRO_0000010222
	Peptide	49 – 55	7	Dermorphin Ref.1		PRO_0000010223
	Propeptide	57 – 82	26			PRO_0000010224
	Peptide	85 – 91	7	Dermorphin Ref.1		PRO_0000010225
	Propeptide	93 – 118	26			PRO_0000010226
	Peptide	121 – 127	7	Dermorphin Ref.1		PRO_0000010227
	Propeptide	129 – 154	26			PRO_0000010228
	Peptide	157 – 163	7	Dermorphin Ref.1		PRO_0000010229
	Propeptide	165 – 186	22			PRO_0000010230
	Peptide	189 – 195	7	[Ile2]-deltorphin Ref.1		PRO_0000010231
	Propeptide	196 – 201	6			PRO_0000010232
Amino acid modifications
	Modified residue	50	1	D-alanine (Ala) By similarity UniProtKB P05422
	Modified residue	55	1	Serine amide By similarity UniProtKB P05422
	Modified residue	86	1	D-alanine (Ala) By similarity UniProtKB P05422
	Modified residue	91	1	Serine amide By similarity UniProtKB P05422
	Modified residue	122	1	D-alanine (Ala) By similarity UniProtKB P05422
	Modified residue	127	1	Serine amide By similarity UniProtKB P05422
	Modified residue	158	1	D-alanine (Ala) By similarity UniProtKB P05422
	Modified residue	163	1	Serine amide By similarity UniProtKB P05422
	Modified residue	190	1	D-allo-isoleucine Probable UniProtKB P05422

Sequences

	Sequence		Length	Mass (Da)
	O93456-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 2F324D6701CDDA31	FASTA	201	23,887
	10 20 30 40 50 60 MSFLKKSLLL ILFLGLVSLS ICEEQKREIE EDENENEEER NEASEMKRYA FGYPSGEEKK 70 80 90 100 110 120 IKRETEEDEN ENEEERNEAS EMKRYAFGYP SGEEKKIKRE TEEDENENEE ERNEASEMKR 130 140 150 160 170 180 YAFGYPSGEE KKIKRETEED ENENEEERNE GSEMKRYAFG YPSGGAKRMK RKPVEEEENH 190 200 VAGSKMKRYI FHLMDGEVKK I

References

[1]	"A new opioid peptide predicted from cloned cDNAs from skin of Pachymedusa dacnicolor and Agalychnis annae." Wechselberger C., Severini C., Kreil G., Negri L. FEBS Lett. 429:41-43(1998) [PubMed: 9657380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF ILE[2]-DELTORPHIN. Tissue: Skin.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ005443 mRNA. Translation: CAA06546.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	O93456.
Family and domain databases
InterPro	IPR004275. Brevinin. [Graphical view]
Pfam	PF03032. Brevenin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEM_PACDA

Accession

Primary (citable) accession number: O93456

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)