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Protein

Glycine receptor subunit alphaZ1

Gene

glra1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:10188956, PubMed:26344198). Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (By similarity).By similarity2 Publications

Miscellaneous

Highly sensitive to activation by taurine despite the presence of Val in position 135. In mammals, Val-135 causes a drastic loss of taurine efficacy.1 Publication
The alpha subunit binds strychnine.2 Publications

Enzyme regulationi

Activated by glycine and taurine. Inhibited by strychnine (PubMed:10188956, PubMed:26344198). Allosterically activated by ivermectin (PubMed:26344198). Inhibited by picrotoxinin (PubMed:26344198). Strychnine binding locks the channel in a closed conformation and prevents channel opening in response to extracellular glycine (PubMed:26344198). Can also be activated by GABA and inhibited by bicuculline, but this requires heterologous expression in human cells (PubMed:10188956).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi216ZincBy similarity1
Metal bindingi218ZincBy similarity1
Metal bindingi239ZincBy similarity1
Sitei285Important for obstruction of the ion pore in the closed conformation1 Publication1

GO - Molecular functioni

GO - Biological processi

  • cellular response to amino acid stimulus Source: UniProtKB
  • cellular response to ethanol Source: UniProtKB
  • cellular response to zinc ion Source: UniProtKB
  • central nervous system development Source: ZFIN
  • ion transport Source: ZFIN
  • neuropeptide signaling pathway Source: GO_Central
  • protein homooligomerization Source: ZFIN
  • regulation of neuron differentiation Source: ZFIN
  • response to amino acid Source: ZFIN
  • synaptic transmission, glycinergic Source: GO_Central

Keywordsi

Molecular functionChloride channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandChloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-DRE-112314 Neurotransmitter receptors and postsynaptic signal transmission

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alphaZ1
Gene namesi
Name:glra1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 14

Organism-specific databases

ZFINiZDB-GENE-991117-1 glra1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 246Extracellular1 PublicationAdd BLAST222
Transmembranei247 – 268Helical; Name=11 PublicationAdd BLAST22
Topological domaini269 – 273Cytoplasmic1 Publication5
Transmembranei274 – 294Helical; Name=21 PublicationAdd BLAST21
Topological domaini295 – 305Extracellular1 PublicationAdd BLAST11
Transmembranei306 – 326Helical; Name=31 PublicationAdd BLAST21
Topological domaini327 – 412Cytoplasmic1 PublicationAdd BLAST86
Transmembranei413 – 433Helical; Name=41 PublicationAdd BLAST21
Topological domaini434 – 444Extracellular1 PublicationAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000041525 – 444Glycine receptor subunit alphaZ1Add BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1 Publication1
Disulfide bondi162 ↔ 176By similarity
Disulfide bondi222 ↔ 2331 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO93430
PRIDEiO93430

PTM databases

iPTMnetiO93430

Expressioni

Tissue specificityi

Expressed in brain.1 Publication

Gene expression databases

BgeeiENSDARG00000012019
ExpressionAtlasiO93430 baseline

Interactioni

Subunit structurei

Homopentamer (in vitro) (PubMed:26344198). Heteropentamer composed of glra1 and glrb (By similarity). Both homopentamers and heteropentamers form functional ion channels (By similarity).By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-61772N
STRINGi7955.ENSDARP00000066196

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JADelectron microscopy3.90A/B/C/D/E34-434[»]
3JAEelectron microscopy3.90A/B/C/D/E34-434[»]
3JAFelectron microscopy3.80A/B/C/D/E34-434[»]
ProteinModelPortaliO93430
SMRiO93430
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni226 – 231Strychnine-binding1 Publication6

Domaini

The channel pore is formed by pentameric assembly of the second transmembrane domain from all five subunits. Channel opening is effected by an outward rotation of the transmembrane domains that increases the diameter of the pore.1 Publication

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643 Eukaryota
ENOG410XPWH LUCA
GeneTreeiENSGT00760000118821
HOGENOMiHOG000231336
HOVERGENiHBG051707
InParanoidiO93430
KOiK05193
OMAiFNFAYGM

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006028 GABAA/Glycine_rcpt
IPR008127 Glycine_rcpt_A
IPR008128 Glycine_rcpt_A1
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00253 GABAARECEPTR
PR01673 GLYRALPHA
PR01674 GLYRALPHA1
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O93430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFALGIYLWE TIVFFSLAAS QQAAARKAAS PMPPSEFLDK LMGKVSGYDA
60 70 80 90 100
RIRPNFKGPP VNVTCNIFIN SFGSIAETTM DYRVNIFLRQ QWNDPRLAYS
110 120 130 140 150
EYPDDSLDLD PSMLDSIWKP DLFFANEKGA NFHEVTTDNK LLRISKNGNV
160 170 180 190 200
LYSIRITLVL ACPMDLKNFP MDVQTCIMQL ESFGYTMNDL IFEWDEKGAV
210 220 230 240 250
QVADGLTLPQ FILKEEKDLR YCTKHYNTGK FTCIEARFHL ERQMGYYLIQ
260 270 280 290 300
MYIPSLLIVI LSWVSFWINM DAAPARVGLG ITTVLTMTTQ SSGSRASLPK
310 320 330 340 350
VSYVKAIDIW MAVCLLFVFS ALLEYAAVNF IARQHKELLR FQRRRRHLKE
360 370 380 390 400
DEAGDGRFSF AAYGMGPACL QAKDGMAIKG NNNNAPTSTN PPEKTVEEMR
410 420 430 440
KLFISRAKRI DTVSRVAFPL VFLIFNIFYW ITYKIIRSED IHKQ
Length:444
Mass (Da):50,766
Last modified:November 1, 1998 - v1
Checksum:i77F6FC8C14E80581
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005812 mRNA Translation: CAA06711.1
RefSeqiNP_571477.1, NM_131402.1
UniGeneiDr.75842

Genome annotation databases

EnsembliENSDART00000005499; ENSDARP00000003268; ENSDARG00000012019
GeneIDi30676
KEGGidre:30676

Similar proteinsi

Entry informationi

Entry nameiGLRA1_DANRE
AccessioniPrimary (citable) accession number: O93430
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health