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Protein

Snaclec convulxin subunit alpha

Gene
N/A
Organism
Crotalus durissus terrificus (South American rattlesnake)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom lectin that activates platelets by binding to the platelet collagen receptor glycoprotein VI (GP6) (PubMed:9153205). The indirect activation of integrin alpha-IIb/beta-3 (ITGA2B/ITGB3) also induced by the toxin is upstream the cytoskeletal translocation of GPIb, FcRgamma (FCER1G) and 14-3-3zeta (YWHAZ)(PubMed:16102113).2 Publications

Keywords - Molecular functioni

Hemostasis impairing toxin, Platelet aggregation activating toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Snaclec convulxin subunit alpha
Short name:
CVX-alpha
OrganismiCrotalus durissus terrificus (South American rattlesnake)
Taxonomic identifieri8732 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeCrotalus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23232 PublicationsAdd
BLAST
Chaini24 – 158135Snaclec convulxin subunit alphaPRO_0000017528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 38
Disulfide bondi55 ↔ 152
Disulfide bondi104 – 104Interchain (with C-100 in subunit beta)
Disulfide bondi127 ↔ 144
Disulfide bondi158 – 158Interchain (with C-26 in subunit beta)

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Tetramer of heterodimers of alpha and beta subunits (alphabeta)4; disulfide-linked.

Structurei

Secondary structure

1
158
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 343Combined sources
Beta strandi37 – 4610Combined sources
Helixi48 – 5811Combined sources
Helixi70 – 8314Combined sources
Beta strandi90 – 923Combined sources
Beta strandi94 – 974Combined sources
Beta strandi100 – 1045Combined sources
Helixi121 – 1233Combined sources
Beta strandi127 – 1315Combined sources
Helixi132 – 1343Combined sources
Beta strandi138 – 1425Combined sources
Beta strandi148 – 1547Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UMRX-ray2.40A/B24-158[»]
1UOSX-ray2.70A/C24-158[»]
ProteinModelPortaliO93426.
SMRiO93426. Positions 24-158.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO93426.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 158125C-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the snaclec family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG004151.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O93426-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRFIFVSFG LLVLFLSLSG TGAGLHCPSD WYYYDQHCYR IFNEEMNWED
60 70 80 90 100
AEWFCTKQAK GAHLVSIKSA KEADFVAWMV TQNIEESFSH VSIGLRVQNK
110 120 130 140 150
EKQCSTKWSD GSSVSYDNLL DLYITKCSLL KKETGFRKWF VASCIGKIPF

VCKFPPQC
Length:158
Mass (Da):18,141
Last modified:November 1, 1998 - v1
Checksum:i949F9C6D673E2318
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16348 mRNA. Translation: CAA76181.1.
AF541882 mRNA. Translation: AAQ11363.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16348 mRNA. Translation: CAA76181.1.
AF541882 mRNA. Translation: AAQ11363.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UMRX-ray2.40A/B24-158[»]
1UOSX-ray2.70A/C24-158[»]
ProteinModelPortaliO93426.
SMRiO93426. Positions 24-158.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004151.

Miscellaneous databases

EvolutionaryTraceiO93426.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of subunits of convulxin, a collagen-like platelet-aggregating protein from Crotalus durissus terrificus venom."
    Leduc M., Bon C.
    Biochem. J. 333:389-393(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-48; 61-68; 108-120; 139-145 AND 149-153.
    Tissue: Venom and Venom gland.
  2. Radis-Baptista G., Camargo A.C.M., Yamane T.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  3. "Platelet activation and signal transduction by convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor."
    Polgar J., Clemetson J.M., Kehrel B.E., Wiedemann M., Magnenat E.M., Wells T.N., Clemetson K.J.
    J. Biol. Chem. 272:13576-13583(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-43, FUNCTION.
    Tissue: Venom.
  4. "Translocation of GPIb and Fc receptor gamma-chain to cytoskeleton in mucetin-activated platelets."
    Lu Q., Clemetson J.M., Clemetson K.J.
    J. Thromb. Haemost. 3:2065-2076(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Crystal structure of the platelet activator convulxin, a disulfide-linked alpha4beta4 cyclic tetramer from the venom of Crotalus durissus terrificus."
    Murakami M.T., Zela S.P., Gava L.M., Michelan-Duarte S., Cintra A.C.O., Arni R.K.
    Biochem. Biophys. Res. Commun. 310:478-482(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 24-158.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 26-158.

Entry informationi

Entry nameiSLA_CRODU
AccessioniPrimary (citable) accession number: O93426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1998
Last modified: October 14, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not act by binding to GPIb.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.