O93403 (CHST3_TORCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbohydrate sulfotransferase 3
EC=2.8.2.17

Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name=C6ST-1
Nervous system involved sulfotransferase

Gene names

Name:	CHST3
Synonyms:	NSIST

Organism

Torpedo californica (Pacific electric ray)

Taxonomic identifier

7787 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Chondrichthyes › Elasmobranchii › Batoidea › Torpediniformes › Torpedinidae › Torpedo

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices By similarity.
Catalytic activity	3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	In electric organ, it is moderately expressed in spinal cord and electric lobe and undetectable in non-neural tissues. Expressed in a punctate distribution in the innervated portion of electrocytes. In the CNS, it is localized within the somas of motor neurons and neurons of the electromotor nucleus. Ref.1
Sequence similarities	Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chondroitin 6-sulfotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Carbohydrate sulfotransferase 3		PRO_0000085192
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 21	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	22 – 441	420	Lumenal Potential
	Nucleotide binding	106 – 112	7	PAPS By similarity
	Nucleotide binding	266 – 274	9	PAPS By similarity
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	221	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O93403 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 993B8B858743BA53	FASTA	441	50,393
	10 20 30 40 50 60 MKMRSKYAII LFFVVALVII EKERNIISRV SDKFTLKFPH AESVAPNNTI SARSLTKNNS 70 80 90 100 110 120 LLANSVAAVW KLLKARRSYS SLQSAASSDV RKVLKGRKHL LLMATTRTGS SFVGEFFNQN 130 140 150 160 170 180 NDIFYLFEPL WHVEKTVTFE PGGMNAVASS IIYRDVVQQL MLCDLYTLEN FLFPMADRHL 190 200 210 220 230 240 TGILFRRGSS KSLCEGEVCT PPKKGGTEKF PCRLRDCGLL NLTLATQACL QKQHVAIKTV 250 260 270 280 290 300 PLRQLEFLRP LVEDFRINLK IIQLVRDPRA VLASRMVAFP SKYNAWKKWA NEGRVPDDDE 310 320 330 340 350 360 VGKIRGNCEN LRATAQLGIS QPPWLKDRFL LMRYEDIALE PVKRAQEMYR FSGIPMTPEV 370 380 390 400 410 420 KKWIYENTQV SKASNNIYST HKISSEQFEK WRLGLPFKIA RVVQQVCEPA MKLFGYKLVK 430 440 DAATLANRSA SLLENRNFWI T

References

[1]

"Expression cloning and characterization of NSIST, a novel sulfotransferase expressed by a subset of neurons and postsynaptic targets." Nastuk M.A., Davis S., Yancopoulos G.D., Fallon J.R. J. Neurosci. 18:7167-7177(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF079875 mRNA. Translation: AAC28491.1.
3D structure databases
ProteinModelPortal	O93403.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG106811.
Family and domain databases
InterPro	IPR016469. Carbohydrate_sulfotransferase. IPR000863. Sulfotransferase_dom. [Graphical view]
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
PIRSF	PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CHST3_TORCA

Accession

Primary (citable) accession number: O93403

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program