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O93403

- CHST3_TORCA

UniProt

O93403 - CHST3_TORCA

Protein

Carbohydrate sulfotransferase 3

Gene

CHST3

Organism
Torpedo californica (Pacific electric ray)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices By similarity.By similarity

    Catalytic activityi

    3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi106 – 1127PAPSBy similarity
    Nucleotide bindingi266 – 2749PAPSBy similarity

    GO - Molecular functioni

    1. chondroitin 6-sulfotransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbohydrate sulfotransferase 3 (EC:2.8.2.17)
    Alternative name(s):
    Chondroitin 6-O-sulfotransferase 1
    Short name:
    C6ST-1
    Nervous system involved sulfotransferase
    Gene namesi
    Name:CHST3
    Synonyms:NSIST
    OrganismiTorpedo californica (Pacific electric ray)
    Taxonomic identifieri7787 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiBatoideaTorpediniformesTorpedinidaeTorpedo

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 441441Carbohydrate sulfotransferase 3PRO_0000085192Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    In electric organ, it is moderately expressed in spinal cord and electric lobe and undetectable in non-neural tissues. Expressed in a punctate distribution in the innervated portion of electrocytes. In the CNS, it is localized within the somas of motor neurons and neurons of the electromotor nucleus.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO93403.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 44CytoplasmicSequence Analysis
    Topological domaini22 – 441420LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2117Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG106811.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view]
    PfamiPF00685. Sulfotransfer_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    O93403-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMRSKYAII LFFVVALVII EKERNIISRV SDKFTLKFPH AESVAPNNTI    50
    SARSLTKNNS LLANSVAAVW KLLKARRSYS SLQSAASSDV RKVLKGRKHL 100
    LLMATTRTGS SFVGEFFNQN NDIFYLFEPL WHVEKTVTFE PGGMNAVASS 150
    IIYRDVVQQL MLCDLYTLEN FLFPMADRHL TGILFRRGSS KSLCEGEVCT 200
    PPKKGGTEKF PCRLRDCGLL NLTLATQACL QKQHVAIKTV PLRQLEFLRP 250
    LVEDFRINLK IIQLVRDPRA VLASRMVAFP SKYNAWKKWA NEGRVPDDDE 300
    VGKIRGNCEN LRATAQLGIS QPPWLKDRFL LMRYEDIALE PVKRAQEMYR 350
    FSGIPMTPEV KKWIYENTQV SKASNNIYST HKISSEQFEK WRLGLPFKIA 400
    RVVQQVCEPA MKLFGYKLVK DAATLANRSA SLLENRNFWI T 441
    Length:441
    Mass (Da):50,393
    Last modified:November 1, 1998 - v1
    Checksum:i993B8B858743BA53
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF079875 mRNA. Translation: AAC28491.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF079875 mRNA. Translation: AAC28491.1 .

    3D structure databases

    ProteinModelPortali O93403.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG106811.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view ]
    Pfami PF00685. Sulfotransfer_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning and characterization of NSIST, a novel sulfotransferase expressed by a subset of neurons and postsynaptic targets."
      Nastuk M.A., Davis S., Yancopoulos G.D., Fallon J.R.
      J. Neurosci. 18:7167-7177(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCHST3_TORCA
    AccessioniPrimary (citable) accession number: O93403
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3