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O93403 (CHST3_TORCA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbohydrate sulfotransferase 3

EC=2.8.2.17
Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name=C6ST-1
Nervous system involved sulfotransferase
Gene names
Name:CHST3
Synonyms:NSIST
OrganismTorpedo californica (Pacific electric ray)
Taxonomic identifier7787 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiBatoideaTorpediniformesTorpedinidaeTorpedo

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices By similarity.

Catalytic activity

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

In electric organ, it is moderately expressed in spinal cord and electric lobe and undetectable in non-neural tissues. Expressed in a punctate distribution in the innervated portion of electrocytes. In the CNS, it is localized within the somas of motor neurons and neurons of the electromotor nucleus. Ref.1

Sequence similarities

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentGolgi apparatus
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionTransferase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionchondroitin 6-sulfotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 441441Carbohydrate sulfotransferase 3
PRO_0000085192

Regions

Topological domain1 – 44Cytoplasmic Potential
Transmembrane5 – 2117Helical; Signal-anchor for type II membrane protein; Potential
Topological domain22 – 441420Lumenal Potential
Nucleotide binding106 – 1127PAPS By similarity
Nucleotide binding266 – 2749PAPS By similarity

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation2211N-linked (GlcNAc...) Potential
Glycosylation4271N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O93403 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 993B8B858743BA53

FASTA44150,393
        10         20         30         40         50         60 
MKMRSKYAII LFFVVALVII EKERNIISRV SDKFTLKFPH AESVAPNNTI SARSLTKNNS 

        70         80         90        100        110        120 
LLANSVAAVW KLLKARRSYS SLQSAASSDV RKVLKGRKHL LLMATTRTGS SFVGEFFNQN 

       130        140        150        160        170        180 
NDIFYLFEPL WHVEKTVTFE PGGMNAVASS IIYRDVVQQL MLCDLYTLEN FLFPMADRHL 

       190        200        210        220        230        240 
TGILFRRGSS KSLCEGEVCT PPKKGGTEKF PCRLRDCGLL NLTLATQACL QKQHVAIKTV 

       250        260        270        280        290        300 
PLRQLEFLRP LVEDFRINLK IIQLVRDPRA VLASRMVAFP SKYNAWKKWA NEGRVPDDDE 

       310        320        330        340        350        360 
VGKIRGNCEN LRATAQLGIS QPPWLKDRFL LMRYEDIALE PVKRAQEMYR FSGIPMTPEV 

       370        380        390        400        410        420 
KKWIYENTQV SKASNNIYST HKISSEQFEK WRLGLPFKIA RVVQQVCEPA MKLFGYKLVK 

       430        440 
DAATLANRSA SLLENRNFWI T 

« Hide

References

[1]"Expression cloning and characterization of NSIST, a novel sulfotransferase expressed by a subset of neurons and postsynaptic targets."
Nastuk M.A., Davis S., Yancopoulos G.D., Fallon J.R.
J. Neurosci. 18:7167-7177(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF079875 mRNA. Translation: AAC28491.1.

3D structure databases

ProteinModelPortalO93403.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG106811.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMSSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Entry information

Entry nameCHST3_TORCA
AccessionPrimary (citable) accession number: O93403
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families