ID   LDHA_CORNI              Reviewed;         332 AA.
AC   O93401;
DT   05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=L-lactate dehydrogenase A chain;
DE            Short=LDH-A;
DE            EC=1.1.1.27;
GN   Name=ldha;
OS   Coryphopterus nicholsi (Blackeye goby) (Rhinogobiops nicholsii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
OC   Acanthomorpha; Acanthopterygii; Percomorpha; Perciformes; Gobioidei;
OC   Gobiidae; Gobiinae; Coryphopterus.
OX   NCBI_TaxID=79888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Muscle;
RX   PubMed=9319749;
RA   Fields P., Somero G.;
RT   "Amino acid sequence differences cannot fully explain interspecific
RT   variation in thermal sensitivities of gobiid fish A4-lactate
RT   dehydrogenases (A4-LDHs).";
RL   J. Exp. Biol. 200:1839-1850(1997).
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; AF079534; AAC31199.1; -; mRNA.
DR   HSSP; P07195; 1I0Z.
DR   SMR; O93401; 2-332.
DR   HOVERGEN; O93401; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:EC.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR011304; L-lactate_DH.
DR   InterPro; IPR018177; L-lactate_DH_AS.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    332       L-lactate dehydrogenase A chain.
FT                                /FTId=PRO_0000168433.
FT   NP_BIND      29     57       NAD (By similarity).
FT   ACT_SITE    193    193       Proton acceptor (By similarity).
FT   BINDING      99     99       NAD (By similarity).
FT   BINDING     106    106       Substrate (By similarity).
FT   BINDING     138    138       NAD or substrate (By similarity).
FT   BINDING     169    169       Substrate (By similarity).
FT   BINDING     248    248       Substrate (By similarity).
SQ   SEQUENCE   332 AA;  36221 MW;  745240067644A705 CRC64;
     MSTKEKLISH VSKEEAVGSR NKVTVVGVGM VGMASAISIL LKDLCDELAL VDVMEDKLKG
     EVMDLQHGSL FLKTHKIVGD KDYSVTANSR VVVVTAGARQ QEGESRLNLV QRNVNIFKFI
     IPNIVKYSPN CILMVVSNPV DILTYVAWKL SGFPRHRVIG SGTNLDSARF RHIMGEKLHL
     HPSSCHGWIV GEHGDSSVPV WSGVNVAGVS LQTLNPKMGA EGDSENWKAV HKMVVDGAYE
     VIKLKGYTSW AIGMSVADLV ESIVKNLHKV HPVSTLVKGM HGVKDEVFLS VPCVLGNSGL
     TDVIHMTLKA DEEKQLVKSA ETLWGVQKEL TL
//