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Protein

Core histone macro-H2A.1

Gene

H2AFY

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The presence of macro-H2A enhances the interaction of the histone octamer with the nucleosomal DNA and may repress the ability of the nucleosome to be remodelled.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Core histone macro-H2A.1
Short name:
Histone macroH2A1
Short name:
mH2A1
Alternative name(s):
H2A.y
H2A/y
Gene namesi
Name:H2AFY
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 13

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 372371Core histone macro-H2A.1PRO_0000227905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki116 – 116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki117 – 117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Post-translational modificationi

Monoubiquitinated at either Lys-116 or Lys-117.By similarity
ADP-ribosylated.Curated

Keywords - PTMi

ADP-ribosylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO93327.
PRIDEiO93327.

Expressioni

Tissue specificityi

Present in liver (at protein level).1 Publication

Developmental stagei

Present in immature erythrocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSGALG00000006360.
ExpressionAtlasiO93327. baseline.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010266.

Structurei

3D structure databases

ProteinModelPortaliO93327.
SMRiO93327. Positions 10-117, 181-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 9076Histone H2AAdd
BLAST
Domaini184 – 370187MacroPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi116 – 16146Lys-richAdd
BLAST

Sequence similaritiesi

Contains 1 histone H2A domain.Curated
Contains 1 Macro domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IV3A. Eukaryota.
KOG1756. Eukaryota.
KOG2633. Eukaryota.
COG2110. LUCA.
COG5262. LUCA.
GeneTreeiENSGT00840000129729.
HOGENOMiHOG000234653.
HOVERGENiHBG009342.
InParanoidiO93327.
KOiK11251.
OMAiKGGKEFT.
OrthoDBiEOG091G0XGD.
PhylomeDBiO93327.
TreeFamiTF332276.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR021171. Core_histone_macro-H2A.
IPR009072. Histone-fold.
IPR002119. Histone_H2A.
IPR007125. Histone_H2A/H2B/H3.
IPR032454. Histone_H2A_C.
IPR002589. Macro_dom.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
PF16211. Histone_H2A_C. 1 hit.
PF01661. Macro. 1 hit.
[Graphical view]
PIRSFiPIRSF037942. Core_histone_macro-H2A. 1 hit.
PRINTSiPR00620. HISTONEH2A.
SMARTiSM00506. A1pp. 1 hit.
SM00414. H2A. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS51154. MACRO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O93327-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA
60 70 80 90 100
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI
110 120 130 140 150
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKTVSKKTG
160 170 180 190 200
GKKGARKSKK KQGEVSKSAS ADSTTEGTPA DGFTVLSTKS LFLGQKLNLI
210 220 230 240 250
HSEISNLAGF EVEAIINPTN ADIDLKDDLG STLEKKGGKE FVEAVIELRK
260 270 280 290 300
KNGPLDIAGA VVSAGHGLPA KFVIHCNSPG WGSDKCEELL EKTVKNCLAL
310 320 330 340 350
ADEKKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY
360 370
FVLFDSESIG IYVQEMAKLD AN
Length:372
Mass (Da):39,656
Last modified:January 23, 2007 - v3
Checksum:i8F5940ECD57C6E95
GO
Isoform 1 (identifier: O93327-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-217: NLIHSEISNLAGFEVEAIIN → QVVQADIATIDSDAVVH
     221-229: ADIDLKDDL → SDFYTGGEV

Show »
Length:369
Mass (Da):39,210
Checksum:iAC0CFB94734E5893
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei198 – 21720NLIHS…EAIIN → QVVQADIATIDSDAVVH in isoform 1. 1 PublicationVSP_017598Add
BLAST
Alternative sequencei221 – 2299ADIDLKDDL → SDFYTGGEV in isoform 1. 1 PublicationVSP_017599

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058445 mRNA. Translation: AAC28846.1.
AF058446 mRNA. Translation: AAC28847.1.
RefSeqiNP_990338.1. NM_205007.1. [O93327-1]
UniGeneiGga.3138.

Genome annotation databases

EnsembliENSGALT00000010280; ENSGALP00000010266; ENSGALG00000006360. [O93327-1]
ENSGALT00000010281; ENSGALP00000010267; ENSGALG00000006360. [O93327-2]
GeneIDi395858.
KEGGigga:395858.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058445 mRNA. Translation: AAC28846.1.
AF058446 mRNA. Translation: AAC28847.1.
RefSeqiNP_990338.1. NM_205007.1. [O93327-1]
UniGeneiGga.3138.

3D structure databases

ProteinModelPortaliO93327.
SMRiO93327. Positions 10-117, 181-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010266.

Proteomic databases

PaxDbiO93327.
PRIDEiO93327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000010280; ENSGALP00000010266; ENSGALG00000006360. [O93327-1]
ENSGALT00000010281; ENSGALP00000010267; ENSGALG00000006360. [O93327-2]
GeneIDi395858.
KEGGigga:395858.

Organism-specific databases

CTDi9555.

Phylogenomic databases

eggNOGiENOG410IV3A. Eukaryota.
KOG1756. Eukaryota.
KOG2633. Eukaryota.
COG2110. LUCA.
COG5262. LUCA.
GeneTreeiENSGT00840000129729.
HOGENOMiHOG000234653.
HOVERGENiHBG009342.
InParanoidiO93327.
KOiK11251.
OMAiKGGKEFT.
OrthoDBiEOG091G0XGD.
PhylomeDBiO93327.
TreeFamiTF332276.

Miscellaneous databases

PROiO93327.

Gene expression databases

BgeeiENSGALG00000006360.
ExpressionAtlasiO93327. baseline.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR021171. Core_histone_macro-H2A.
IPR009072. Histone-fold.
IPR002119. Histone_H2A.
IPR007125. Histone_H2A/H2B/H3.
IPR032454. Histone_H2A_C.
IPR002589. Macro_dom.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
PF16211. Histone_H2A_C. 1 hit.
PF01661. Macro. 1 hit.
[Graphical view]
PIRSFiPIRSF037942. Core_histone_macro-H2A. 1 hit.
PRINTSiPR00620. HISTONEH2A.
SMARTiSM00506. A1pp. 1 hit.
SM00414. H2A. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS51154. MACRO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiH2AY_CHICK
AccessioniPrimary (citable) accession number: O93327
Secondary accession number(s): O93326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.