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Protein

Core histone macro-H2A.1

Gene

H2AFY

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The presence of macro-H2A enhances the interaction of the histone octamer with the nucleosomal DNA and may repress the ability of the nucleosome to be remodelled.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Core histone macro-H2A.1
Short name:
Histone macroH2A1
Short name:
mH2A1
Alternative name(s):
H2A.y
H2A/y
Gene namesi
Name:H2AFY
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002279052 – 372Core histone macro-H2A.1Add BLAST371

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Post-translational modificationi

Monoubiquitinated at either Lys-116 or Lys-117.By similarity
ADP-ribosylated.Curated

Keywords - PTMi

ADP-ribosylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO93327
PRIDEiO93327

Expressioni

Tissue specificityi

Present in liver (at protein level).1 Publication

Developmental stagei

Present in immature erythrocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSGALG00000006360

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010266

Structurei

3D structure databases

ProteinModelPortaliO93327
SMRiO93327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 90Histone H2AAdd BLAST76
Domaini184 – 370MacroPROSITE-ProRule annotationAdd BLAST187

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi116 – 161Lys-richAdd BLAST46

Phylogenomic databases

eggNOGiENOG410IV3A Eukaryota
KOG1756 Eukaryota
KOG2633 Eukaryota
COG2110 LUCA
COG5262 LUCA
GeneTreeiENSGT00910000143981
HOGENOMiHOG000234653
HOVERGENiHBG009342
InParanoidiO93327
KOiK11251
OMAiHCNSPIW
OrthoDBiEOG091G0XGD
PhylomeDBiO93327
TreeFamiTF332276

Family and domain databases

CDDicd00074 H2A, 1 hit
cd02904 Macro_H2A_like, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR021171 Core_histone_macro-H2A
IPR009072 Histone-fold
IPR002119 Histone_H2A
IPR007125 Histone_H2A/H2B/H3
IPR032454 Histone_H2A_C
IPR002589 Macro_dom
IPR035796 Macro_H2A
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PF16211 Histone_H2A_C, 1 hit
PF01661 Macro, 1 hit
PIRSFiPIRSF037942 Core_histone_macro-H2A, 1 hit
PRINTSiPR00620 HISTONEH2A
SMARTiView protein in SMART
SM00506 A1pp, 1 hit
SM00414 H2A, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS51154 MACRO, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O93327-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA
60 70 80 90 100
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI
110 120 130 140 150
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKTVSKKTG
160 170 180 190 200
GKKGARKSKK KQGEVSKSAS ADSTTEGTPA DGFTVLSTKS LFLGQKLNLI
210 220 230 240 250
HSEISNLAGF EVEAIINPTN ADIDLKDDLG STLEKKGGKE FVEAVIELRK
260 270 280 290 300
KNGPLDIAGA VVSAGHGLPA KFVIHCNSPG WGSDKCEELL EKTVKNCLAL
310 320 330 340 350
ADEKKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY
360 370
FVLFDSESIG IYVQEMAKLD AN
Length:372
Mass (Da):39,656
Last modified:January 23, 2007 - v3
Checksum:i8F5940ECD57C6E95
GO
Isoform 1 (identifier: O93327-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-217: NLIHSEISNLAGFEVEAIIN → QVVQADIATIDSDAVVH
     221-229: ADIDLKDDL → SDFYTGGEV

Show »
Length:369
Mass (Da):39,210
Checksum:iAC0CFB94734E5893
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017598198 – 217NLIHS…EAIIN → QVVQADIATIDSDAVVH in isoform 1. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_017599221 – 229ADIDLKDDL → SDFYTGGEV in isoform 1. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058445 mRNA Translation: AAC28846.1
AF058446 mRNA Translation: AAC28847.1
RefSeqiNP_990338.1, NM_205007.1 [O93327-1]
UniGeneiGga.3138

Genome annotation databases

EnsembliENSGALT00000069960; ENSGALP00000054035; ENSGALG00000040141 [O93327-1]
GeneIDi395858
KEGGigga:395858

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiH2AY_CHICK
AccessioniPrimary (citable) accession number: O93327
Secondary accession number(s): O93326
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health