O93299 (CP2K3_ONCMY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2K3
EC=1.14.14.1

Alternative name(s):
CYPIIK3
Cytochrome P450 LMC2

Gene names

Name:

cyp2k3

Organism

Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Taxonomic identifier

8022 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonoidei › Salmonidae › Salmoninae › Oncorhynchus

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Microsome membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Cytochrome P450 2K3		PRO_0000051774
Sites
	Metal binding	434	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O93299 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 2194A1054FFE928E	FASTA	491	55,288
	10 20 30 40 50 60 MSLIEGLLQT SSTVTLLGTV LFLLVLYLRS SGSSSEGQGK EPPGPRPLPL LGNMLQLDLK 70 80 90 100 110 120 KPYCTLCELS KKYGSIFTFH FGPKKVVVLA GYKTVKQALV NQAEDFGDRD ITPVFYDFNQ 130 140 150 160 170 180 GHGILFANGD SWKEMRRFAL TNLRDFGMGK KGSEEKILEE IPYLIEVFEK HEGKAFDTTQ 190 200 210 220 230 240 SVLYAVSNII SAIVYGSRFE YTDPLFTGMA DRAKESIHLT GSASIQMYNM FPWLGPWINN 250 260 270 280 290 300 LTRLKKNIAD MKMEVTELVR GLKETLNPHM CRGFVDSFLV RKQTLEESGH MDSFYHDDNL 310 320 330 340 350 360 VFSVGNLFSA GTDTTGTTLR WGLLLMTKYP HIQDQVQEEI SGVIGSRQTL VEDRKNLPYT 370 380 390 400 410 420 DAVIHETQRL ANIAPMSIPH TTSRDVTFQG YFIKKDDSEW ESPHTLTPSH FLDEKGGFVK 430 440 450 460 470 480 RDAFMAFSAG RRVCLGEGLA RMELFLFFTS LLQHFRFSPP PGVTEDDLDL TPSVEFTHNP 490 SPHQLCAVSR V

References

[1]

"Cloning, sequencing and heterologous expression of CYP2K1 and CYP2K3 from sexually mature rainbow trout liver and their roles in aflatoxin B1 activation." Yang Y.-H., Miranda C.L., Wang J.-L., Buhler D.R. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043551 mRNA. Translation: AAC26494.1.
3D structure databases
ProteinModelPortal	O93299.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2K3_ONCMY

Accession

Primary (citable) accession number: O93299

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	March 6, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program