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O93295

- ENTP8_CHICK

UniProt

O93295 - ENTP8_CHICK

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Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene
ENTPD8
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside ATPases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides.2 Publications

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.1 Publication

Cofactori

Ca2+ or Mg2+. Has lower efficiency with Mg2+ By similarity.

Kineticsi

  1. KM=0.51 mM for ATP1 Publication
  2. KM=5.3 mM for ADP

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Proton acceptor By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
Short name:
E-NTPDase 8
Short name:
NTPDase 8
Short name:
NTPDase8
Alternative name(s):
Liver ecto-ATP diphosphohydrolase
Gene namesi
Name:ENTPD8
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic Reviewed prediction
Transmembranei8 – 2821Helical; Reviewed predictionAdd
BLAST
Topological domaini29 – 463435Extracellular Reviewed predictionAdd
BLAST
Transmembranei464 – 48623Helical; Reviewed predictionAdd
BLAST
Topological domaini487 – 4937Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000209905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi65 – 651N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi76 ↔ 100 By similarity
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi133 – 1331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi223 – 2231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi234 – 2341N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi244 ↔ 291 By similarity
Glycosylationi267 – 2671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi324 – 3241N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi327 ↔ 333 By similarity
Glycosylationi330 – 3301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi361 – 3611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi372 – 3721N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi379 ↔ 401 By similarity
Glycosylationi382 – 3821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi445 – 4451N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO93295.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000014500.

Structurei

3D structure databases

ProteinModelPortaliO93295.

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiQ90X66.
KOiK01510.
PhylomeDBiO93295.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O93295-1 [UniParc]FASTAAdd to Basket

« Hide

MEYKGKVVAG LLTATCVFSI IALILSAVDV KDVFLPPGTK YGLVFDAGST    50
HTALYVYQWP ADKENGTGIV SQVESCTVNG SGISSYADDP AGAGASLKPC 100
LDKAMAVIPV EQQWQTPTYL GATAGMRLLR EQNSTKAEQV FAEVSKAIRE 150
FPVDFRGAQI LTGNEEGSFG WITVNYLLET LIKFSFAGKW EHPQNTEVLG 200
ALDLGGASTQ ITFQPGVTIE DKNTSVLFRL YGTNYSLYTH SYLCYGQIQA 250
SKRLMAALHQ DGSYVQNISH PCYPKGYRRI ITIAEIYDSP CVPTPSMLSP 300
AQILTVTGTG NPAACPTAIL KLFNLTCGAN RTCGFDGVYQ PPVRGQFFAF 350
AGFYYTFSFL NLTGQQSLSH VNATVWDFCN KNWSELVETF PQNKEHLHTY 400
CVVGLYILTL LVDGYKFDEH TWSNIHFSQK AGNADIGWTL GFMLNLTNMI 450
PTEALEHVKG HEPSLWAGAI SFIVLAIVAG LVAILLQCFW KSK 493
Length:493
Mass (Da):54,035
Last modified:November 1, 1998 - v1
Checksum:iF14FF4C3AA2F3603
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161C → W AA sequence 1 Publication
Sequence conflicti21 – 211I → G AA sequence 1 Publication
Sequence conflicti278 – 2803RRI → QEN in AAL25086. 1 Publication
Sequence conflicti316 – 3161P → R in AAL25086. 1 Publication
Sequence conflicti325 – 3251L → F in AAL25086. 1 Publication
Sequence conflicti461 – 4622HE → QQ in AAL25086. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041355 mRNA. Translation: AAC26491.1.
AF426405 mRNA. Translation: AAL25086.1.
RefSeqiNP_989578.1. NM_204247.1.
UniGeneiGga.49226.

Genome annotation databases

GeneIDi374095.
KEGGigga:374095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041355 mRNA. Translation: AAC26491.1 .
AF426405 mRNA. Translation: AAL25086.1 .
RefSeqi NP_989578.1. NM_204247.1.
UniGenei Gga.49226.

3D structure databases

ProteinModelPortali O93295.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000014500.

Proteomic databases

PaxDbi O93295.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 374095.
KEGGi gga:374095.

Organism-specific databases

CTDi 377841.

Phylogenomic databases

eggNOGi COG5371.
HOGENOMi HOG000059572.
HOVERGENi HBG018982.
InParanoidi Q90X66.
KOi K01510.
PhylomeDBi O93295.

Miscellaneous databases

NextBioi 20813614.
PROi O93295.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the chicken oviduct ecto-ATP-diphosphohydrolase."
    Nagy A.K., Knowles A.F., Nagami G.T.
    J. Biol. Chem. 273:16043-16049(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-21 AND 150-156.
    Tissue: Oviduct.
  2. "Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase."
    Knowles A.F., Nagy A.K., Strobel R.S., Wu-Weis M.
    Eur. J. Biochem. 269:2373-2382(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Tissue: Liver.
  3. "Immunolocalization of the ecto-ATPase and ecto-apyrase in chicken gizzard and stomach. Purification and N-terminal sequence of the stomach ecto-apyrase."
    Lewis-Carl S., Kirley T.L.
    J. Biol. Chem. 272:23645-23652(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-17.
    Tissue: Stomach.
  4. "Either the carboxyl- or the amino-terminal region of the human ecto-ATPase (E-NTPDase 2) confers detergent and temperature sensitivity to the chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8)."
    Mukasa T., Lee Y., Knowles A.F.
    Biochemistry 44:11160-11170(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiENTP8_CHICK
AccessioniPrimary (citable) accession number: O93295
Secondary accession number(s): Q90X66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3