O93279 (A4_TAKRU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amyloid beta A4 protein

Alternative name(s):
ABPP
Short name=APP
Alzheimer disease amyloid A4 protein homolog

Cleaved into the following chain:

1. Beta-amyloid protein
   Alternative name(s):
   A-beta
   Beta-APP

Gene names

Name:

app

Organism

Takifugu rubripes (Japanese pufferfish) (Fugu rubripes) [Reference proteome]

Taxonomic identifier

31033 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Tetraodontiformes › Tetraodontoidei › Tetradontoidea › Tetraodontidae › Takifugu

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O) By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the APP family. Contains 1 BPTI/Kunitz inhibitor domain.

Ontologies

Keywords
Cellular component	Amyloid Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	heparin binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 737	719	Amyloid beta A4 protein		PRO_0000000197
	Chain	639 – 681	43	Beta-amyloid protein Potential		PRO_0000000198
Regions
	Topological domain	19 – 668	650	Extracellular Potential
	Transmembrane	669 – 689	21	Helical; Potential
	Topological domain	690 – 737	48	Cytoplasmic Potential
	Domain	286 – 344	59	BPTI/Kunitz inhibitor
	Region	726 – 729	4	Clathrin-binding By similarity
Sites
	Metal binding	148	1	Copper By similarity
	Metal binding	152	1	Copper By similarity
	Metal binding	169	1	Copper By similarity
	Site	300 – 301	2	Reactive bond
Amino acid modifications
	Glycosylation	522	1	N-linked (GlcNAc...) Potential
	Disulfide bond	39 ↔ 63		By similarity
	Disulfide bond	74 ↔ 118		By similarity
	Disulfide bond	99 ↔ 106		By similarity
	Disulfide bond	134 ↔ 188		By similarity
	Disulfide bond	145 ↔ 175		By similarity
	Disulfide bond	159 ↔ 187		By similarity
	Disulfide bond	290 ↔ 340		By similarity
	Disulfide bond	299 ↔ 323		By similarity
	Disulfide bond	315 ↔ 336		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O93279 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 6FAD01E2E3B2B7E2	FASTA	737	82,857
	10 20 30 40 50 60 MGETTAFVLL LVATLTRSSE IPADDTVGLL TEPQVAMFCG KLNMHINVQN GKWESDPSGT 70 80 90 100 110 120 KSCLNTKEGI LQYCQEVYPE LQITNVVEAN QPVSIQNWCK KGRKQCRSHT HIVVPYRCLV 130 140 150 160 170 180 GEFVSDALLV PDKCKFLHQE RMNQCESHLH WHTVAKESCG DRSMNLHDYG MLLPCGIDRF 190 200 210 220 230 240 RGVKFVCCPA ETEQETDSSE VEGEESDVWW GGADPEYSEN SPPTPSRATY VAGDAFERDE 250 260 270 280 290 300 NGDGDEDEED DEDVDPTDEQ ESDERTANVA MTTTTTTTTE SVEEVVRAVC WAQAESGPCR 310 320 330 340 350 360 AMLERWYFNP KKRRCVPFLF GGCGGNRNNF ESEEYCLAVC SSSLPTVAPS PPDAVDQYFE 370 380 390 400 410 420 APGDDNEHAD FRKAKESLEA KHRERMSQVM REWEEAERQA KNLPRADKKA VIQHFQEKVE 430 440 450 460 470 480 ALEQEAAGER QQLVETHMAR VEALLNSRRR LTLENYLGAL QANPPRARQV LSLLKKYVRA 490 500 510 520 530 540 EQKDRQHTLK HYEHVRTVDP KKAAQIRPQV LTHLRVIDER MNQSLALLYK VPSVASEIQN 550 560 570 580 590 600 QIYPAAGSDC KDPVEHCVCP QVDGLVSYGN DALMPDQAYS SAPMDMGVDG LGSIDQSFNQ 610 620 630 640 650 660 ANTENHVEPV DARPIPDRGL PTRPVSSLKL EEMPEVRTET DKRQSAGYEV YHQKLVFFAD 670 680 690 700 710 720 DVGSNKGAII GLMVGGVVIA TVIVITLVML RKKQYTSIHH GVIEVDAAVT PEERHLARMQ 730 QNGYENPTYK FFEQMQN

References

[1]	"Analysis of pufferfish homologues of the AT-rich human APP gene." Villard L., Tassone F., Crnogorac-Jurcevic T., Clancy K., Gardiner K. Gene 210:17-24(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF090120 Genomic DNA. Translation: AAD13392.1.
3D structure databases
ProteinModelPortal	O93279.
SMR	O93279. Positions 29-124, 354-542, 643-680, 706-737.
ModBase	Search...
Protein-protein interaction databases
STRING	31033.ENSTRUP00000027587.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG289770.
Family and domain databases
Gene3D	3.30.1490.140. 1 hit. 3.90.570.10. 1 hit. 4.10.230.10. 1 hit. 4.10.410.10. 1 hit.
InterPro	IPR008155. Amyloid_glyco. IPR013803. Amyloid_glyco_Abeta. IPR011178. Amyloid_glyco_Cu-bd. IPR024329. Amyloid_glyco_E2_domain. IPR008154. Amyloid_glyco_extra. IPR019744. Amyloid_glyco_extracell_CS. IPR015849. Amyloid_glyco_heparin-bd. IPR019745. Amyloid_glyco_intracell_CS. IPR019543. APP_amyloid_C. IPR002223. Prot_inh_Kunz-m. IPR020901. Prtase_inh_Kunz-CS. [Graphical view]
Pfam	PF10515. APP_amyloid. 1 hit. PF12924. APP_Cu_bd. 1 hit. PF12925. APP_E2. 1 hit. PF02177. APP_N. 1 hit. PF03494. Beta-APP. 1 hit. PF00014. Kunitz_BPTI. 1 hit. [Graphical view]
PRINTS	PR00203. AMYLOIDA4. PR00759. BASICPTASE. PR00204. BETAAMYLOID.
SMART	SM00006. A4_EXTRA. 1 hit. SM00131. KU. 1 hit. [Graphical view]
SUPFAM	SSF56491. A4_extra. 1 hit. SSF89811. Amyloid_glyco_Cu-bd. 1 hit. SSF57362. Prot_inh_Kunz-m. 1 hit. SSF109843. SSF109843. 1 hit.
PROSITE	PS00319. A4_EXTRA. 1 hit. PS00320. A4_INTRA. 1 hit. PS00280. BPTI_KUNITZ_1. 1 hit. PS50279. BPTI_KUNITZ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4_TAKRU

Accession

Primary (citable) accession number: O93279

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program