Frizzled-8 precursor - fzd8 - Xenopus laevis (African clawed frog)

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O93274 (FZD8_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Frizzled-8
Short name=Fz-8
Short name=Xfz8

Gene names

Name:	fzd8
Synonyms:	fz8

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt8, Wnt5A or Wnt3A induces expression of beta-catenin target genes. Displays an axis-inducing activity. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein By similarity Ref.3.
Developmental stage	First expressed at high levels in the late blastula stages. At early gastrula, expressed in the deep cells of the Spemann organizer prior to involution of the dorsal blastopore lip. Detected in presumptive neurectoderm as gastrulation proceeds. Becomes restricted to the anterior ectoderm by the end of gastrulation. At neurula stages, localized in the most anterior region of the embryo, mainly in the anterior ectoderm including telencephalic and cement gland regions.
Domain	Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity. The FZ domain is involved in binding with Wnt ligands By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	canonical Wnt signaling pathway Inferred from direct assay PubMed 18945944. Source: BHF-UCL multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 18945944. Source: BHF-UCL
Cellular_component	integral component of membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction PubMed 16086323. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FRIED	Q6EHH9	2	EBI-7735236,EBI-7735259

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

Potential

Chain

24 – 581

558

Frizzled-8

PRO_0000013002

Regions

Topological domain

24 – 239

216

Extracellular Potential

Transmembrane

240 – 260

Helical; Name=1; Potential

Topological domain

261 – 271

Cytoplasmic Potential

Transmembrane

272 – 292

Helical; Name=2; Potential

Topological domain

293 – 320

Extracellular Potential

Transmembrane

321 – 341

Helical; Name=3; Potential

Topological domain

342 – 377

Cytoplasmic Potential

Transmembrane

378 – 398

Helical; Name=4; Potential

Topological domain

399 – 407

Extracellular Potential

Transmembrane

408 – 428

Helical; Name=5; Potential

Topological domain

429 – 454

Cytoplasmic Potential

Transmembrane

455 – 475

Helical; Name=6; Potential

Topological domain

476 – 505

Extracellular Potential

Transmembrane

506 – 526

Helical; Name=7; Potential

Topological domain

527 – 581

Cytoplasmic Potential

Domain

24 – 144

121

Region

64 – 71

Palmitate-binding groove By similarity

Region

88 – 93

Wnt-binding By similarity

Region

140 – 146

Wnt-binding By similarity

Motif

529 – 534

Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity

Motif

579 – 581

PDZ-binding

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

146

N-linked (GlcNAc...) Potential

Disulfide bond

28 ↔ 89

By similarity

Disulfide bond

36 ↔ 82

By similarity

Disulfide bond

73 ↔ 111

By similarity

Disulfide bond

100 ↔ 141

By similarity

Disulfide bond

104 ↔ 128

By similarity

Experimental info

Sequence conflict

1 – 3

MES → MECPY Ref.2

Sequence conflict

S → L in AAC77361. Ref.2

Sequence conflict

L → V in AAC77361. Ref.2

Sequence conflict

W → G in AAC77361. Ref.2

Sequence conflict

C → S in AAC77361. Ref.2

Sequence conflict

135

G → S in AAC77361. Ref.2

Sequence conflict

171

G → S in AAC77361. Ref.2

Sequence conflict

175

V → A in AAC77361. Ref.2

Sequence conflict

185

T → P in AAC77361. Ref.2

Sequence conflict

216

I → T in AAC77361. Ref.2

Sequence conflict

237

E → D in AAC77361. Ref.2

Sequence conflict

494 – 496

PEM → SEG in AAC77361. Ref.2

Sequence conflict

500

H → R in AAC77361. Ref.2

Sequence conflict

547

A → T in AAC77361. Ref.2

Sequence conflict

565

G → A in AAC77361. Ref.2

Sequence conflict

572

C → Y in AAC77361. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

O93274 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 80890C408AB21E23
Show »

FASTA

581

65,379

        10         20         30         40         50         60 
MESLSLSLLL LVSWLQGSQC AAAKELSCQE ITVPLCKDIG YNYTYMPNQF NHDTQDEAGM 

        70         80         90        100        110        120 
EVHQFWPLVV IHCSPDLKFF LCSMYTPICL EDYKKPLPPC RSVCERARAG CAPLMRQYGF 

       130        140        150        160        170        180 
AWPDRMRCDR LPEQGNPDTL CMDYYNRTEQ TTAAPSHPEP PKPPARSVPK GRTRVEPPRS 

       190        200        210        220        230        240 
RSRATGCESG CQCRAPMVQV SNERHPLYNR VRTGQIPNCA MPCHNPFFSP EERTFTEFWI 

       250        260        270        280        290        300 
GLWSVLCFAS TFATVSTFLI DMERFKYPER PIIFLSACYL LVSTGYLIRL IAGHEKVACS 

       310        320        330        340        350        360 
RGELDLEHII HYETTGPALC TLVFLLIYFF GMASSIWWVI LSLTWFLAAG MKWGNEAIAG 

       370        380        390        400        410        420 
YSQYFHLAAW LVPSIKSIAV LALSSVDGDP VAGICFVGNQ NLDNLRGFVL APLVIYLFIG 

       430        440        450        460        470        480 
SMFLLAGFVS LFRIRSVIKQ GGTKTDKLEK LMIRIGIFSV LYTVPATIVV ACFFYEQHNR 

       490        500        510        520        530        540 
QGWEVAHNCN SCQPEMAQPH RPDYAVFMLK YFMCLVVGIT SGVWIWSGKT LESWRAFCTR 

       550        560        570        580 
CCWGSKATGG SMYSDVSTGL TWRSGTGSSV SCPKQMPLSQ V

« Hide

References

[1]	"A role for Xenopus Frizzled 8 in dorsal development." Itoh K., Jacob J., Sokol S.Y. Mech. Dev. 74:145-157(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], COUPLING TO BETA-CATENIN PATHWAY. Tissue: Embryo.
[2]	"Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis." Deardorff M.A., Tan C., Conrad L.J., Klein P.S. Development 125:2687-2700(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[3]	"Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein." Hsieh J.C., Rattner A., Smallwood P.M., Nathans J. Proc. Natl. Acad. Sci. U.S.A. 96:3546-3551(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017177 mRNA. Translation: AAC31121.1. AF033110 mRNA. Translation: AAC77361.1.
RefSeq	NP_001079144.1. NM_001085675.1. NP_001084206.1. NM_001090737.1.
UniGene	Xl.293. Xl.412.
3D structure databases
ProteinModelPortal	O93274.
SMR	O93274. Positions 25-144.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
IntAct	O93274. 1 interaction.
MINT	MINT-1893946.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	373690. 399367.
KEGG	xla:373690. xla:399367.
Organism-specific databases
CTD	373690. 399367.
Xenbase	XB-GENE-865411. fzd8.
Phylogenomic databases
HOVERGEN	HBG006977.
KO	K02375.
Family and domain databases
Gene3D	1.10.2000.10. 1 hit.
InterPro	IPR000539. Frizzled. IPR015526. Frizzled/SFRP. IPR020067. Frizzled_dom. IPR017981. GPCR_2-like. [Graphical view]
PANTHER	PTHR11309. PTHR11309. 1 hit.
Pfam	PF01534. Frizzled. 1 hit. PF01392. Fz. 1 hit. [Graphical view]
PRINTS	PR00489. FRIZZLED.
SMART	SM00063. FRI. 1 hit. [Graphical view]
SUPFAM	SSF63501. SSF63501. 1 hit.
PROSITE	PS50038. FZ. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FZD8_XENLA

Accession

Primary (citable) accession number: O93274
Secondary accession number(s): Q9YI55

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	November 1, 1998
Last modified:	June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents