Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O93274 (FZD8_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Frizzled-8

Short name=Fz-8
Short name=Xfz8
Gene names
Name:fzd8
Synonyms:fz8
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length581 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt8, Wnt5A or Wnt3A induces expression of beta-catenin target genes. Displays an axis-inducing activity. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein By similarity Ref.3.

Developmental stage

First expressed at high levels in the late blastula stages. At early gastrula, expressed in the deep cells of the Spemann organizer prior to involution of the dorsal blastopore lip. Detected in presumptive neurectoderm as gastrulation proceeds. Becomes restricted to the anterior ectoderm by the end of gastrulation. At neurula stages, localized in the most anterior region of the embryo, mainly in the anterior ectoderm including telencephalic and cement gland regions.

Domain

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity.

The FZ domain is involved in binding with Wnt ligands By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FRIEDQ6EHH92EBI-7735236,EBI-7735259

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 581558Frizzled-8
PRO_0000013002

Regions

Topological domain24 – 239216Extracellular Potential
Transmembrane240 – 26021Helical; Name=1; Potential
Topological domain261 – 27111Cytoplasmic Potential
Transmembrane272 – 29221Helical; Name=2; Potential
Topological domain293 – 32028Extracellular Potential
Transmembrane321 – 34121Helical; Name=3; Potential
Topological domain342 – 37736Cytoplasmic Potential
Transmembrane378 – 39821Helical; Name=4; Potential
Topological domain399 – 4079Extracellular Potential
Transmembrane408 – 42821Helical; Name=5; Potential
Topological domain429 – 45426Cytoplasmic Potential
Transmembrane455 – 47521Helical; Name=6; Potential
Topological domain476 – 50530Extracellular Potential
Transmembrane506 – 52621Helical; Name=7; Potential
Topological domain527 – 58155Cytoplasmic Potential
Domain24 – 144121FZ
Region64 – 718Palmitate-binding groove By similarity
Region88 – 936Wnt-binding By similarity
Region140 – 1467Wnt-binding By similarity
Motif529 – 5346Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity
Motif579 – 5813PDZ-binding

Amino acid modifications

Glycosylation421N-linked (GlcNAc...) Potential
Glycosylation1461N-linked (GlcNAc...) Potential
Disulfide bond28 ↔ 89 By similarity
Disulfide bond36 ↔ 82 By similarity
Disulfide bond73 ↔ 111 By similarity
Disulfide bond100 ↔ 141 By similarity
Disulfide bond104 ↔ 128 By similarity

Experimental info

Sequence conflict1 – 33MES → MECPY Ref.2
Sequence conflict71S → L in AAC77361. Ref.2
Sequence conflict101L → V in AAC77361. Ref.2
Sequence conflict141W → G in AAC77361. Ref.2
Sequence conflict201C → S in AAC77361. Ref.2
Sequence conflict1351G → S in AAC77361. Ref.2
Sequence conflict1711G → S in AAC77361. Ref.2
Sequence conflict1751V → A in AAC77361. Ref.2
Sequence conflict1851T → P in AAC77361. Ref.2
Sequence conflict2161I → T in AAC77361. Ref.2
Sequence conflict2371E → D in AAC77361. Ref.2
Sequence conflict494 – 4963PEM → SEG in AAC77361. Ref.2
Sequence conflict5001H → R in AAC77361. Ref.2
Sequence conflict5471A → T in AAC77361. Ref.2
Sequence conflict5651G → A in AAC77361. Ref.2
Sequence conflict5721C → Y in AAC77361. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O93274 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 80890C408AB21E23

FASTA58165,379
        10         20         30         40         50         60 
MESLSLSLLL LVSWLQGSQC AAAKELSCQE ITVPLCKDIG YNYTYMPNQF NHDTQDEAGM 

        70         80         90        100        110        120 
EVHQFWPLVV IHCSPDLKFF LCSMYTPICL EDYKKPLPPC RSVCERARAG CAPLMRQYGF 

       130        140        150        160        170        180 
AWPDRMRCDR LPEQGNPDTL CMDYYNRTEQ TTAAPSHPEP PKPPARSVPK GRTRVEPPRS 

       190        200        210        220        230        240 
RSRATGCESG CQCRAPMVQV SNERHPLYNR VRTGQIPNCA MPCHNPFFSP EERTFTEFWI 

       250        260        270        280        290        300 
GLWSVLCFAS TFATVSTFLI DMERFKYPER PIIFLSACYL LVSTGYLIRL IAGHEKVACS 

       310        320        330        340        350        360 
RGELDLEHII HYETTGPALC TLVFLLIYFF GMASSIWWVI LSLTWFLAAG MKWGNEAIAG 

       370        380        390        400        410        420 
YSQYFHLAAW LVPSIKSIAV LALSSVDGDP VAGICFVGNQ NLDNLRGFVL APLVIYLFIG 

       430        440        450        460        470        480 
SMFLLAGFVS LFRIRSVIKQ GGTKTDKLEK LMIRIGIFSV LYTVPATIVV ACFFYEQHNR 

       490        500        510        520        530        540 
QGWEVAHNCN SCQPEMAQPH RPDYAVFMLK YFMCLVVGIT SGVWIWSGKT LESWRAFCTR 

       550        560        570        580 
CCWGSKATGG SMYSDVSTGL TWRSGTGSSV SCPKQMPLSQ V 

« Hide

References

[1]"A role for Xenopus Frizzled 8 in dorsal development."
Itoh K., Jacob J., Sokol S.Y.
Mech. Dev. 74:145-157(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], COUPLING TO BETA-CATENIN PATHWAY.
Tissue: Embryo.
[2]"Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis."
Deardorff M.A., Tan C., Conrad L.J., Klein P.S.
Development 125:2687-2700(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[3]"Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein."
Hsieh J.C., Rattner A., Smallwood P.M., Nathans J.
Proc. Natl. Acad. Sci. U.S.A. 96:3546-3551(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF017177 mRNA. Translation: AAC31121.1.
AF033110 mRNA. Translation: AAC77361.1.
RefSeqNP_001079144.1. NM_001085675.1.
NP_001084206.1. NM_001090737.1.
UniGeneXl.293.
Xl.412.

3D structure databases

ProteinModelPortalO93274.
SMRO93274. Positions 25-144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO93274. 1 interaction.
MINTMINT-1893946.

Protein family/group databases

GPCRDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID373690.
399367.
KEGGxla:373690.
xla:399367.

Organism-specific databases

CTD373690.
399367.
XenbaseXB-GENE-865411. fzd8.

Phylogenomic databases

HOVERGENHBG006977.
KOK02375.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFZD8_XENLA
AccessionPrimary (citable) accession number: O93274
Secondary accession number(s): Q9YI55
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries