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Protein

Replicase large subunit

Gene
N/A
Organism
Tobacco mosaic virus (strain OM) (TMV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Replicase large subunit: is an RNA-dependent RNA polymerase active in viral RNA replication.By similarity
Replicase small subunit: is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity).By similarityCurated

Miscellaneous

This protein is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the terminator codon TGA occurs between the codons for Gln-1116 and Gln-1118, this results in the addition of the RdRp region to the replicase.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi833 – 840ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Suppressor of RNA silencing, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
183 kDa protein
RNA-directed RNA polymerase
Cleaved into the following chain:
Alternative name(s):
126 kDa protein
Methyltransferase/RNA helicase
Short name:
MT/HEL
OrganismiTobacco mosaic virus (strain OM) (TMV)
Taxonomic identifieri12251 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageVirgaviridaeTobamovirus
Virus hostiNicotiana tabacum (Common tobacco) [TaxID: 4097]
Proteomesi
  • UP000000280 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000411681 – 1616Replicase large subunitAdd BLAST1616
ChainiPRO_00000411691 – 1116Replicase small subunitAdd BLAST1116

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 281Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST210
Domaini801 – 963(+)RNA virus helicase ATP-bindingAdd BLAST163
Domaini964 – 1116(+)RNA virus helicase C-terminalAdd BLAST153
Domaini1380 – 1493RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 458MethyltransferaseSequence analysisAdd BLAST409
Regioni830 – 1085HelicaseSequence analysisAdd BLAST256

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG09000048.

Family and domain databases

InterProiView protein in InterPro
IPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
PfamiView protein in Pfam
PF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O93058-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYTQTATTS ALLDTVRGNN SLVNDLAKRR LYDTAVEEFN ARDRRPKVNF
60 70 80 90 100
SKVISEEQTL IATRAYPEFQ ITFYNTQNAV HSLAGGLRSL ELEYLMMQIP
110 120 130 140 150
YGSLTYDIGG NFASHLFKGR AYVHCCMPNL DVRDIMRHEG QKDSIELYLS
160 170 180 190 200
RLERGGKTVP NFQKEAFDRY AEIPEDAVCH NTFQTCEHQP MQQSGRVYAI
210 220 230 240 250
ALHSIYDIPA DEFGAALLRK NVHTCYAAFH FSENLLLEDS YVNLDEINAC
260 270 280 290 300
FSRDGDKLTF SFASESTLNY CHSYSNILKY VCKTYFPASN REVYMKEFLV
310 320 330 340 350
TRVNTWFCKF SRIDTFLLYK GVAHKSVDSE QFYTAMEDAW HYKKTLAMCN
360 370 380 390 400
SERILLEDSS SVNYWFPKMR DMVIVPLFDI SLETSKRTRK EVLVSKDFVF
410 420 430 440 450
TVLNHIRTYQ AKALTYANVL SFVESIRSRV IINGVTARSE WDVDKSLLQS
460 470 480 490 500
LSMTFYLHTK LAVLKDDLLI SKFSLGSKTV CQHVWDEISL AFGNAFPSVK
510 520 530 540 550
ERLLNRKLIR VAGDALEIRV PDLYVTFHDR LVTEYKASVD MPALDIRKKM
560 570 580 590 600
EETEVMYNAL SELSVLRESD KFDVDVFSQM CQSLEVDPMT AAKVIVAVMS
610 620 630 640 650
NESGLTLTFE RPTEANVALA LQDQEKASEG ALVVTSREVE EPSMKGSMAR
660 670 680 690 700
GELQLAGLAG DHPESSYSRN EEIESLEQFH MATADSLIRK QMSSIVYTGP
710 720 730 740 750
IKVQQMKNFI DSLVASLSAA VSNLVKILKD TAAIDLETRQ KFGVLDVASR
760 770 780 790 800
KWLIKPTAKS HAWGVVETHA RKYHVALLEY DEQGVVTCDD WRRVAVSSES
810 820 830 840 850
VVYSDMAKLR TLRRLLRNGE PHVSSAKVVL VDGVPGCGKT KEILSRVNFD
860 870 880 890 900
EDLILVPGKQ AAEMIRRRAN SSGIIVATKD NVKTVDSFMM NFGKSTRCQF
910 920 930 940 950
KRLFIDEGLM LHTGCVNFLV AMSLCEIAYV YGDTQQIPYI NRVSGFPYPA
960 970 980 990 1000
HFAKLEVDEV ETRRTTLRCP ADVTHYLNRR YEGFVMSTSS VKKSVSQEMV
1010 1020 1030 1040 1050
GGAAVINPIS KPLHGKILTF TQSDKEALLS RGYSDVHTVH EVQGETYSDV
1060 1070 1080 1090 1100
SLVRLTPTPV SIIAGDSPHV LVALSRHTCS LKYYTVVMDP LVSIIRDLEK
1110 1120 1130 1140 1150
LSSYLLDMYK VDAGTQXQLQ IDSVFKGSNL FVAAPKTGDI SDMQFYYDKC
1160 1170 1180 1190 1200
LPGNSTMMNN FDAVTMRLTD ISLNVKDCIL DMSKSVAAPK DQIKPLTPMV
1210 1220 1230 1240 1250
RTAAEMPRQT GLLENLVAMI KRNFNAPELS GIIDIENTAS LVVDKFFDSY
1260 1270 1280 1290 1300
LLKEKRKPNK NVSLFSRESL NRWLEKQEQV TIGQLADFDF VDLPAVDQYR
1310 1320 1330 1340 1350
HMIKAQPKQK LDTSIQTEYP ALQTIVYHSK KINAIFGPLF SELTRQLLDS
1360 1370 1380 1390 1400
VDSSRFLFFT RKTPAQIEDF FGDLDSHVPM DVLELDISKY DKSQNEFHCA
1410 1420 1430 1440 1450
VEYEIWRRLG FEDFLGEVWK QGHRKTTLKD YTAGIKTCIW YQRKSGDVTT
1460 1470 1480 1490 1500
FIGNTVIIAA CLASMLPMEK IIKGAFCGDD SLLYFPKGCE FPDVQHSANL
1510 1520 1530 1540 1550
MWNFEAKLFK KQYGYFCGRY VIHHDRGCIV YYDPLKLISK LGAKHIKDWE
1560 1570 1580 1590 1600
HLEEFRRSLC DVAVSLNNCA YYTQLDDAVR EVHKTAPPGS FVYKSLVKFL
1610
SDKVLFRSLF IDGSSC
Length:1,616
Mass (Da):183,332
Last modified:November 1, 1998 - v1
Checksum:i1D4E01831E24BDBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78608 Genomic RNA. Translation: BAA11429.1.
D78608 Genomic RNA. Translation: BAA11430.1.

Keywords - Coding sequence diversityi

RNA suppression of termination

Similar proteinsi

Entry informationi

Entry nameiRDRP_TMVOM
AccessioniPrimary (citable) accession number: O93058
Secondary accession number(s): Q88561
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: April 12, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families