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Protein

Gag-Pol polyprotein

Gene
N/A
Organism
Human immunodeficiency virus 1
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein predictedi

Functioni

Catalytic activityi

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.SAAS annotation
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).SAAS annotation
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.SAAS annotation

Cofactori

Mg2+SAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl proteaseSAAS annotation, DNA-directed DNA polymeraseSAAS annotation, EndonucleaseSAAS annotation, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymeraseSAAS annotation, Transferase

Keywords - Biological processi

DNA integration, DNA recombinationSAAS annotation, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virusSAAS annotation, Host gene expression shutoff by virus, Host-virus interaction, Viral genome integrationSAAS annotation, Viral penetration into host nucleusSAAS annotation, Virion maturationSAAS annotation, Virus entry into host cell, Virus exit from host cell

Keywords - Ligandi

DNA-bindingSAAS annotation, MagnesiumSAAS annotation, Metal-binding, RNA-bindingSAAS annotation, Viral nucleoproteinUniRule annotation, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag-Pol polyproteinSAAS annotation
OrganismiHuman immunodeficiency virus 1Imported
Taxonomic identifieri11676 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusPrimate lentivirus group
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

  • Host cell membrane SAAS annotation; Lipid-anchor SAAS annotation
  • Host cytoplasm SAAS annotation
  • Host nucleus SAAS annotation
  • Virion UniRule annotation
  • Host nucleus UniRule annotation
  • Host cytoplasm UniRule annotation
  • Virion SAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid proteinUniRule annotationSAAS annotation, Host cell membraneSAAS annotation, Host cytoplasmUniRule annotationSAAS annotation, Host membrane, Host nucleusUniRule annotationSAAS annotation, Membrane, Virion

PTM / Processingi

Post-translational modificationi

Gag-Pol polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO92651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini389 – 40416CCHC-typeInterPro annotationAdd
BLAST
Domaini411 – 42616CCHC-typeInterPro annotationAdd
BLAST
Domaini506 – 57570Peptidase A2InterPro annotationAdd
BLAST
Domaini629 – 819191Reverse transcriptaseInterPro annotationAdd
BLAST
Domaini1019 – 1142124RNase HInterPro annotationAdd
BLAST
Domaini1148 – 118942Integrase-typeInterPro annotationAdd
BLAST
Domaini1199 – 1349151Integrase catalyticInterPro annotationAdd
BLAST
Domaini1368 – 141548Integrase-type DNA-bindingInterPro annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili94 – 11724Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 reverse transcriptase domain.UniRule annotation
Contains CCHC-type zinc fingers.SAAS annotation
Contains RNase H domain.SAAS annotation
Contains integrase catalytic domain.SAAS annotation
Contains integrase-type DNA-binding domain.SAAS annotation
Contains integrase-type zinc finger.SAAS annotation
Contains peptidase A2 domain.SAAS annotation

Keywords - Domaini

Coiled coilSequence analysis, RepeatSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
1.10.1200.30. 1 hit.
1.10.150.90. 1 hit.
1.10.375.10. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
4.10.60.10. 1 hit.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR000721. Gag_p24.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR000071. Lentvrl_matrix_N.
IPR012344. Matrix_HIV/RSV.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR018061. Retropepsins.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00540. Gag_p17. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
PRINTSiPR00234. HIV1MATRIX.
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50158. ZF_CCHC. 2 hits.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O92651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGARASVLSG GKFDAWEKIR LRPGGKKKYR MKHLIWASRE LDRFALNPSL
60 70 80 90 100
LETADGCQQI MEKLQPALKT GTEEIKSLFN TVATLYCVHQ RIDVKDTKEA
110 120 130 140 150
LDKIEEIKNK SKQKTQQAAA DTGNSSNVSQ NYPIVQNAQG QMIHQSLSPR
160 170 180 190 200
TLNAWVKVIE EKAFSPEVIP MFSALSEGAT PQDLNMMLNI VGGHQAAMQM
210 220 230 240 250
LKDTINEEAA EWDRLHPVHA GPIPPGQMRE PRGSDIAGTT STPQEQIGWM
260 270 280 290 300
TGNPPIPVGD IYKRWIILGL NKIVRMYSPV SILDIKQGPK EPFRDYVDRF
310 320 330 340 350
FKTFRAEQAT QDVKNWMTDT LLVQNANPDC KAILRALGPG ATLEEMMTAC
360 370 380 390 400
QGVGGPGHKA RVLAEAMSQV QHTNIMMQRG NFKGQKRIKC FNCGKEGHLA
410 420 430 440 450
RNCRALRKKG CWKCGKEGHQ MKDCTERQAN FFRENLAFQK GEAREFSSEQ
460 470 480 490 500
TGTNSSTSRD LWDGGRDSLP SEAGAERQGT GPTLSFPQIT LWQRPLVTVR
510 520 530 540 550
IGGQLKEALL DTGADDTVLE DINLPGKWKP KMIGGIGGFI KVKQYDQILI
560 570 580 590 600
EICGKKAIGT VLVGPTPVNI IGRNMLTQIG CTLNFPISPI ETVPVKLKPG
610 620 630 640 650
MDGPKVKQWP LTEEKIKALT EICTEMEKEG KISKIGPENP YNTPIFAIKK
660 670 680 690 700
KDSTKWRKLV DFRELNKRTQ DFWEVQLGIP HPAGLKKKKS VTVLDVGDAY
710 720 730 740 750
FSVPLHEEFR KYTAFTIPST NNETPGVRYQ YNVLPQGWKG SPAIFQSSMT
760 770 780 790 800
KILEPFRSKN PEIVIYQYMD DLYVGSDLEI GQHRAKIEEL RAHLLSWGLI
810 820 830 840 850
TPDKKHQKEP PFLWMGYELH PDKWTVQPIE LPEKDSWTVN DIQKLVGKLN
860 870 880 890 900
WASQIYAGIK VKQLCKLLRG AKALTDVVTL TEEAELELAE NREILKEPVH
910 920 930 940 950
GVYYDPSKDL IAEIQKQGQD QWTYQIFQEP FKNLKTGKYA RKRSAHTNDV
960 970 980 990 1000
RQLAEVVQKV VMESIVIWGK TPKFRLPIQK ETWDTWWMDY WQATWILEWE
1010 1020 1030 1040 1050
FVNTPPLVKL WYQLEKDPIA GAETFYVDGA ANRETKLGKA GYVTDKGRQK
1060 1070 1080 1090 1100
VVSLTETTNQ KTELHAIHLA LQDSGSEVNI VTDSQYALGI IQAQPDKSES
1110 1120 1130 1140 1150
EIVNQIIEKL IEKDKVYLSW VPAHKGIGGN EQVDKLVSSG IRKVLFLDGI
1160 1170 1180 1190 1200
DKAQEEHERY HSNWRTMASD FNLPPIVAKE IVASCDKCQL KGEAMHGQVD
1210 1220 1230 1240 1250
CSPGIWQLDC THLEGKVIVV AVHVASGYIE AEVIPAETGQ ETAYFLLKLA
1260 1270 1280 1290 1300
GRWPVKIVHT DNGSNFTSAA VKAACWWANI QQEFGIPYNP QSQGVVESMN
1310 1320 1330 1340 1350
KELKKIIGQV REQAEHLKTA VQMAVFIHNF KRKGGIGGYS AGERIIDIIA
1360 1370 1380 1390 1400
TDIQTKELQK HITKIQNFRV YYRDSRDPLW KGPAKLFWKG EGAVVIQDNS
1410 1420 1430
DIKVVPRRKA KIIRDYGKQM AGDDCVAGRQ DED
Length:1,433
Mass (Da):161,473
Last modified:November 1, 1998 - v1
Checksum:iF8678F30124C5352
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004885 Genomic DNA. Translation: AAC97532.1.

Keywords - Coding sequence diversityi

Ribosomal frameshiftingSAAS annotation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004885 Genomic DNA. Translation: AAC97532.1.

3D structure databases

ProteinModelPortaliO92651.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
1.10.1200.30. 1 hit.
1.10.150.90. 1 hit.
1.10.375.10. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
4.10.60.10. 1 hit.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR000721. Gag_p24.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR000071. Lentvrl_matrix_N.
IPR012344. Matrix_HIV/RSV.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR018061. Retropepsins.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00540. Gag_p17. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
PRINTSiPR00234. HIV1MATRIX.
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50158. ZF_CCHC. 2 hits.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Subtypes of human immunodeficiency virus type 1 and disease stage among women in Nairobi, Kenya."
    Neilson J.R., John G.C., Carr J.K., Lewis P., Kreiss J.K., Jackson S., Nduati R.W., Mbori-Ngacha D., Panteleeff D.D., Bodrug S., Giachetti C., Bott M.A., Richardson B.A., Bwayo J., Ndinya-Achola J., Overbaugh J.
    J. Virol. 73:4393-4403(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Q23-CxCImported.
  2. "Variants from the diverse virus population identified at seroconversion of a clade A human immunodeficiency virus type 1-infected woman have distinct biological properties."
    Poss M., Overbaugh J.
    J. Virol. 73:5255-5264(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Q23-CxCImported.

Entry informationi

Entry nameiO92651_9HIV1
AccessioniPrimary (citable) accession number: O92651
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Multifunctional enzymeSAAS annotation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.