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O92367

- HEMA_CVMDV

UniProt

O92367 - HEMA_CVMDV

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Murine coronavirus (strain DVIM) (MHV-DVIM) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei44 – 441NucleophileBy similarity
    Active sitei334 – 3341Charge relay systemBy similarity
    Active sitei337 – 3371Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    ORF Names:2b
    OrganismiMurine coronavirus (strain DVIM) (MHV-DVIM) (Murine hepatitis virus)
    Taxonomic identifieri231423 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiMus [TaxID: 10088]

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 431408Hemagglutinin-esterasePRO_0000037146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi48 ↔ 69By similarity
    Glycosylationi53 – 531N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi93 – 931N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi117 ↔ 166By similarity
    Glycosylationi151 – 1511N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi157 – 1571N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi199 – 1991N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi205 ↔ 284By similarity
    Disulfide bondi213 ↔ 257By similarity
    Glycosylationi244 – 2441N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi248 – 2481N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi315 ↔ 320By similarity
    Glycosylationi324 – 3241N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi352 – 3521N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi355 ↔ 379By similarity
    Glycosylationi366 – 3661N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

    Structurei

    3D structure databases

    ProteinModelPortaliO92367.
    SMRiO92367. Positions 25-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 399376Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini421 – 43111IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei400 – 42021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 131121Esterase domain first partBy similarityAdd
    BLAST
    Regioni132 – 274143Receptor bindingBy similarityAdd
    BLAST
    Regioni275 – 387113Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O92367-1 [UniParc]FASTAAdd to Basket

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    MARTDAMAPR TLLLVLSLGY AFGFNEPLNV VSHLNDDWFL FGDSRSDCNH    50
    INNLSQQNYN YMDINPELCK SGKISAKAGN SLFKSFHFTD FYNYTGEGSQ 100
    IIFYEGVNFT PYVGFKCLNN GDNNRWMGNK ARFYTQLYQK MAHYRSLSVI 150
    NITYTYNGSA GPVSMCKHIA NGVTLTLNNP TFIGKEVSKP DYYYESEANF 200
    TLQGCDEFIV PLCVFNGQYL SSKLYYDDSQ YYYNVDTGVL YGFNSTLNIT 250
    SGLDLTCIYL ALTPGNYISI SNELLLTVPS KAICLRKPKA FTPVQVVDSR 300
    WHSNRQSDNM TAIACQLPYC YFRNTTSDYN GVYDSHHGDA GFTSILAGLM 350
    YNVSCLAQQG AFVYNNVSSS WPQYPYGHCP TAANIVFMAP VCMYDPLPVI 400
    LLGVLLGIAV LIIVFLMFYF MTDSGVRLHE A 431
    Length:431
    Mass (Da):48,440
    Last modified:November 1, 1998 - v1
    Checksum:iDF4BE9EE0AE7301B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF091734 mRNA. Translation: AAC63044.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF091734 mRNA. Translation: AAC63044.1 .

    3D structure databases

    ProteinModelPortali O92367.
    SMRi O92367. Positions 25-384.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the hemagglutinin-esterase (HE) genes of two enterotropic MHV strains."
      Compton S.R., Moore K.M.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
      Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
      J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiHEMA_CVMDV
    AccessioniPrimary (citable) accession number: O92367
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3