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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Murine coronavirus (strain DVIM) (MHV-DVIM) (Murine hepatitis virus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.UniRule annotation

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44NucleophileUniRule annotation1
Active sitei334Charge relay systemUniRule annotation1
Active sitei337Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esteraseUniRule annotation (EC:3.1.1.53UniRule annotation)
Short name:
HE proteinUniRule annotation
Alternative name(s):
E3 glycoproteinUniRule annotation
Gene namesi
Name:HEUniRule annotation
ORF Names:2b
OrganismiMurine coronavirus (strain DVIM) (MHV-DVIM) (Murine hepatitis virus)
Taxonomic identifieri231423 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus [TaxID: 10088]

Subcellular locationi

  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 399Virion surfaceUniRule annotationAdd BLAST378
Transmembranei400 – 420HelicalUniRule annotationAdd BLAST21
Topological domaini421 – 431IntravirionUniRule annotationAdd BLAST11

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotationAdd BLAST21
ChainiPRO_000003714622 – 431Hemagglutinin-esteraseUniRule annotationAdd BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi48 ↔ 69UniRule annotation
Glycosylationi53N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi93N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi117 ↔ 166UniRule annotation
Glycosylationi151N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi157N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi199N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi205 ↔ 284UniRule annotation
Disulfide bondi213 ↔ 257UniRule annotation
Glycosylationi244N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi248N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi309N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi315 ↔ 320UniRule annotation
Glycosylationi324N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi352N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi355 ↔ 379UniRule annotation
Glycosylationi366N-linked (GlcNAc...) asparagine; by hostUniRule annotation1

Post-translational modificationi

N-glycosylated in the host RER.UniRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO92367

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.UniRule annotation

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 36Combined sources4
Beta strandi38 – 42Combined sources5
Helixi44 – 46Combined sources3
Helixi48 – 53Combined sources6
Helixi66 – 70Combined sources5
Beta strandi74 – 76Combined sources3
Helixi82 – 87Combined sources6
Beta strandi88 – 90Combined sources3
Beta strandi99 – 104Combined sources6
Turni111 – 113Combined sources3
Helixi123 – 140Combined sources18
Turni141 – 143Combined sources3
Beta strandi144 – 151Combined sources8
Beta strandi157 – 159Combined sources3
Beta strandi167 – 169Combined sources3
Beta strandi174 – 177Combined sources4
Beta strandi179 – 183Combined sources5
Helixi191 – 193Combined sources3
Beta strandi197 – 202Combined sources6
Beta strandi204 – 218Combined sources15
Beta strandi229 – 234Combined sources6
Turni235 – 237Combined sources3
Beta strandi239 – 244Combined sources6
Beta strandi254 – 262Combined sources9
Beta strandi264 – 270Combined sources7
Beta strandi276 – 278Combined sources3
Beta strandi281 – 288Combined sources8
Beta strandi294 – 297Combined sources4
Helixi310 – 314Combined sources5
Turni317 – 319Combined sources3
Beta strandi320 – 323Combined sources4
Beta strandi332 – 334Combined sources3
Helixi340 – 346Combined sources7
Helixi347 – 350Combined sources4
Beta strandi354 – 357Combined sources4
Beta strandi360 – 363Combined sources4
Beta strandi365 – 367Combined sources3
Beta strandi376 – 378Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JIFX-ray2.00A/B24-395[»]
ProteinModelPortaliO92367
SMRiO92367
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 131Esterase domain 1UniRule annotationAdd BLAST121
Regioni132 – 274Receptor bindingUniRule annotationAdd BLAST143
Regioni275 – 387Esterase domain 2UniRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.UniRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_04207 BETA_CORONA_HE, 1 hit
InterProiView protein in InterPro
IPR008980 Capsid_hemagglutn
IPR007142 Hemagglutn-estrase_core
IPR003860 Hemagglutn-estrase_hemagglutn
PfamiView protein in Pfam
PF03996 Hema_esterase, 1 hit
PF02710 Hema_HEFG, 1 hit
SUPFAMiSSF49818 SSF49818, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O92367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTDAMAPR TLLLVLSLGY AFGFNEPLNV VSHLNDDWFL FGDSRSDCNH
60 70 80 90 100
INNLSQQNYN YMDINPELCK SGKISAKAGN SLFKSFHFTD FYNYTGEGSQ
110 120 130 140 150
IIFYEGVNFT PYVGFKCLNN GDNNRWMGNK ARFYTQLYQK MAHYRSLSVI
160 170 180 190 200
NITYTYNGSA GPVSMCKHIA NGVTLTLNNP TFIGKEVSKP DYYYESEANF
210 220 230 240 250
TLQGCDEFIV PLCVFNGQYL SSKLYYDDSQ YYYNVDTGVL YGFNSTLNIT
260 270 280 290 300
SGLDLTCIYL ALTPGNYISI SNELLLTVPS KAICLRKPKA FTPVQVVDSR
310 320 330 340 350
WHSNRQSDNM TAIACQLPYC YFRNTTSDYN GVYDSHHGDA GFTSILAGLM
360 370 380 390 400
YNVSCLAQQG AFVYNNVSSS WPQYPYGHCP TAANIVFMAP VCMYDPLPVI
410 420 430
LLGVLLGIAV LIIVFLMFYF MTDSGVRLHE A
Length:431
Mass (Da):48,440
Last modified:November 1, 1998 - v1
Checksum:iDF4BE9EE0AE7301B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091734 mRNA Translation: AAC63044.1

Similar proteinsi

Entry informationi

Entry nameiHEMA_CVMDV
AccessioniPrimary (citable) accession number: O92367
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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