SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O92367

- HEMA_CVMDV

UniProt

O92367 - HEMA_CVMDV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hemagglutinin-esterase

Gene
HE, 2b
Organism
Murine coronavirus (strain DVIM) (MHV-DVIM) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441Nucleophile By similarity
Active sitei236 – 2361Charge relay system By similarity
Active sitei337 – 3371Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiMurine coronavirus (strain DVIM) (MHV-DVIM) (Murine hepatitis virus)
Taxonomic identifieri231423 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus [TaxID: 10088]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 399376Virion surface Reviewed predictionAdd
BLAST
Transmembranei400 – 42021Helical; Reviewed predictionAdd
BLAST
Topological domaini421 – 43111Intravirion Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 431408Hemagglutinin-esterasePRO_0000037146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 69 By similarity
Glycosylationi53 – 531N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi93 – 931N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi117 ↔ 166 By similarity
Glycosylationi151 – 1511N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi157 – 1571N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi199 – 1991N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi205 ↔ 284 By similarity
Disulfide bondi213 ↔ 257 By similarity
Glycosylationi244 – 2441N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi248 – 2481N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi309 – 3091N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi315 ↔ 320 By similarity
Glycosylationi324 – 3241N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi352 – 3521N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi355 ↔ 379 By similarity
Glycosylationi366 – 3661N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliO92367.
SMRiO92367. Positions 25-384.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 131121Esterase domain first part By similarityAdd
BLAST
Regioni132 – 274143Receptor binding By similarityAdd
BLAST
Regioni275 – 387113Esterase domain second part By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O92367-1 [UniParc]FASTAAdd to Basket

« Hide

MARTDAMAPR TLLLVLSLGY AFGFNEPLNV VSHLNDDWFL FGDSRSDCNH    50
INNLSQQNYN YMDINPELCK SGKISAKAGN SLFKSFHFTD FYNYTGEGSQ 100
IIFYEGVNFT PYVGFKCLNN GDNNRWMGNK ARFYTQLYQK MAHYRSLSVI 150
NITYTYNGSA GPVSMCKHIA NGVTLTLNNP TFIGKEVSKP DYYYESEANF 200
TLQGCDEFIV PLCVFNGQYL SSKLYYDDSQ YYYNVDTGVL YGFNSTLNIT 250
SGLDLTCIYL ALTPGNYISI SNELLLTVPS KAICLRKPKA FTPVQVVDSR 300
WHSNRQSDNM TAIACQLPYC YFRNTTSDYN GVYDSHHGDA GFTSILAGLM 350
YNVSCLAQQG AFVYNNVSSS WPQYPYGHCP TAANIVFMAP VCMYDPLPVI 400
LLGVLLGIAV LIIVFLMFYF MTDSGVRLHE A 431
Length:431
Mass (Da):48,440
Last modified:November 1, 1998 - v1
Checksum:iDF4BE9EE0AE7301B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF091734 mRNA. Translation: AAC63044.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF091734 mRNA. Translation: AAC63044.1 .

3D structure databases

ProteinModelPortali O92367.
SMRi O92367. Positions 25-384.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence of the hemagglutinin-esterase (HE) genes of two enterotropic MHV strains."
    Compton S.R., Moore K.M.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
    Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
    J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_CVMDV
AccessioniPrimary (citable) accession number: O92367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi