O91729 (NTP1_CFEPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:

NPH1

Organism

Choristoneura fumiferana entomopoxvirus (CfEPV)

Taxonomic identifier

28322 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae › Betaentomopoxvirus

Virus host

Choristoneura fumiferana (Spruce budworm) [TaxID: 7141]

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	Nucleoside triphosphatase I		PRO_0000099101
Regions
	Domain	48 – 212	165	Helicase ATP-binding
	Domain	378 – 541	164	Helicase C-terminal
	Nucleotide binding	61 – 68	8	ATP By similarity
	Region	467 – 533	67	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	150 – 153	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	O91729 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 430815EC765CD1FD	FASTA	647	76,048
	10 20 30 40 50 60 MFALDSIVGK HINYALDKTQ HLPNKINNSI TNTEIILQDY QYFASRIFIG LKNLNSMLLF 70 80 90 100 110 120 WDTGTGKTLT AVYIIKYIKE LFPRWIILIF IKKSLYVDPW LNTISSYISD TSNIKFIYYD 130 140 150 160 170 180 STSSLDKFNN IYRSIESSLN KKNRLLIIID EVHKLISRSV KKDNSERNFT PIYRKLIKLA 190 200 210 220 230 240 NYENNKILCM SATPITNNIA EFNNLIGLLR PNVMNIKEEY INNGKLINFK EIRETLLGIC 250 260 270 280 290 300 SYKRLIEADS LTDTNYIDGY AKKSIFYHNI IMSDEQSKLY NMAERYDYKT ELGGLKTMRR 310 320 330 340 350 360 LISSFAFYDL KIKGDLDNVE YNEMIKRKLA EFSEFTKNIN FSKEFINAFK NNEIKTKTDL 370 380 390 400 410 420 LITDINNYNI LYQYSCKYIE ACRIILNSRG KVLLFEPLVN FEGISSLKYY FNCFNISYIE 430 440 450 460 470 480 YSSKTIKMRD NDLNYYNNYE NNDGNKIKVC IFSYAGSEGI SFKCINDIII LDMPWNESEL 490 500 510 520 530 540 KQIIGRSIRL NSHEYLPINY RYVNVHFIIS YSNNRKSVDK EILDIIKNKQ GKINVVFDLL 550 560 570 580 590 600 KASPIEIIHN MYKYIEPVDN EIIFETIRKT RMKEMNISNV IINLKLYPIT YCKDYDRATI 610 620 630 640 LKGLLNKDTN IIYDNDTPVA ILLVDNNNLP IFVIENDILI YITNDYY

References

[1]	"Characterization of the nucleoside triphosphate phosphohydrolase I gene from the Choristoneura fumiferana entomopoxvirus." Li X., Wallis J.L., Barrett J.W., Krell P.J., Arif B.M. Virus Res. 56:93-105(1998) [PubMed: 9784068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF027657 Genomic DNA. Translation: AAC63435.1.
3D structure databases
ProteinModelPortal	O91729.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_CFEPV

Accession

Primary (citable) accession number: O91729

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program