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O91262

- HEMA_CVPV

UniProt

O91262 - HEMA_CVPV

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Protein

Hemagglutinin-esterase

Gene
HE
Organism
Puffinosis coronavirus (PV) (Puffinosis virus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Nucleophile By similarity
Active sitei238 – 2381Charge relay system By similarity
Active sitei345 – 3451Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiPuffinosis coronavirus (PV) (Puffinosis virus)
Taxonomic identifieri76583 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiPuffinus puffinus (Manx shearwater) [TaxID: 48688]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 407383Virion surface Reviewed predictionAdd
BLAST
Transmembranei408 – 42821Helical; Reviewed predictionAdd
BLAST
Topological domaini429 – 43911Intravirion Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 439415Hemagglutinin-esterasePRO_0000037151Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 70 By similarity
Glycosylationi94 – 941N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi118 ↔ 167 By similarity
Glycosylationi152 – 1521N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi196 – 1961N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi202 ↔ 291 By similarity
Disulfide bondi210 ↔ 264 By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi316 – 3161N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi322 ↔ 327 By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi337 – 3371N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi360 – 3601N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi363 ↔ 387 By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliO91262.
SMRiO91262. Positions 26-392.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 132121Esterase domain first part By similarityAdd
BLAST
Regioni133 – 281149Receptor binding By similarityAdd
BLAST
Regioni282 – 395114Esterase domain second part By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O91262-1 [UniParc]FASTAAdd to Basket

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MGSMCIAMAP RTLLLLIGCQ LALGFNEPLN VVSHLSDDWF LFGDSRSDCS    50
YVENNGHPAF DWLDLPQELC HSGKISAKSG NSLFKSFHFT DWYNYTGEGD 100
QVIFYEGVNF SPSHGFKCLA EGDNKRWMGN KARFYALVYK KMAYYRSLSF 150
VNVSYSYGGK AKPTAICKDN TLTLNNPTFI SKESNYVDYY YESDANFTLE 200
GCDEFIVPLC VFNGHSRGSS SDPANKYYMD SQMYYNMDTG VFYGFNSTLD 250
VGNTAQNPGL DLTCIYYALT PGNYKAVSLE YLLTIPSKAI CLRKPKRFMP 300
VQVVDSRWNN AKHSDNMTAV ACQTPYCLFR NTSSGYNGST HDVHHGGFHF 350
RKLLSGLLYN VSCIAQQGAF FYNNVSSQWP VLGYGQCPTA ANIEFIAPVC 400
LYDPLPVILL GVLLGIAVLI IVFLLFYFMT DSGVRLHEA 439
Length:439
Mass (Da):49,180
Last modified:November 1, 1998 - v1
Checksum:iA578474DCBAAF7C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005960 mRNA. Translation: CAA06776.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005960 mRNA. Translation: CAA06776.1 .

3D structure databases

ProteinModelPortali O91262.
SMRi O91262. Positions 26-392.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Identification of a coronavirus hemagglutinin-esterase with a substrate specificity different from those of influenza C virus and bovine coronavirus."
    Klausegger A., Strobl B., Regl G., Kaser A., Luytjes W., Vlasak R.
    J. Virol. 73:3737-3743(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The sialate-4-O-acetylesterases of coronaviruses related to mouse hepatitis virus: a proposal to reorganize group 2 Coronaviridae."
    Wurzer W.J., Obojes K., Vlasak R.
    J. Gen. Virol. 83:395-402(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_CVPV
AccessioniPrimary (citable) accession number: O91262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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