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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Puffinosis coronavirus (PV) (Puffinosis virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451NucleophileBy similarity
Active sitei342 – 3421Charge relay systemBy similarity
Active sitei345 – 3451Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiPuffinosis coronavirus (PV) (Puffinosis virus)
Taxonomic identifieri76583 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiPuffinus puffinus (Manx shearwater) [TaxID: 48688]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 407383Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei408 – 42821HelicalSequence AnalysisAdd
BLAST
Topological domaini429 – 43911IntravirionSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 439415Hemagglutinin-esterasePRO_0000037151Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 70By similarity
Glycosylationi94 – 941N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi118 ↔ 167By similarity
Glycosylationi152 – 1521N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi196 – 1961N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi202 ↔ 291By similarity
Disulfide bondi210 ↔ 264By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi322 ↔ 327By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi337 – 3371N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi360 – 3601N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi363 ↔ 387By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliO91262.
SMRiO91262. Positions 26-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 132121Esterase domain first partBy similarityAdd
BLAST
Regioni133 – 281149Receptor bindingBy similarityAdd
BLAST
Regioni282 – 395114Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O91262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSMCIAMAP RTLLLLIGCQ LALGFNEPLN VVSHLSDDWF LFGDSRSDCS
60 70 80 90 100
YVENNGHPAF DWLDLPQELC HSGKISAKSG NSLFKSFHFT DWYNYTGEGD
110 120 130 140 150
QVIFYEGVNF SPSHGFKCLA EGDNKRWMGN KARFYALVYK KMAYYRSLSF
160 170 180 190 200
VNVSYSYGGK AKPTAICKDN TLTLNNPTFI SKESNYVDYY YESDANFTLE
210 220 230 240 250
GCDEFIVPLC VFNGHSRGSS SDPANKYYMD SQMYYNMDTG VFYGFNSTLD
260 270 280 290 300
VGNTAQNPGL DLTCIYYALT PGNYKAVSLE YLLTIPSKAI CLRKPKRFMP
310 320 330 340 350
VQVVDSRWNN AKHSDNMTAV ACQTPYCLFR NTSSGYNGST HDVHHGGFHF
360 370 380 390 400
RKLLSGLLYN VSCIAQQGAF FYNNVSSQWP VLGYGQCPTA ANIEFIAPVC
410 420 430
LYDPLPVILL GVLLGIAVLI IVFLLFYFMT DSGVRLHEA
Length:439
Mass (Da):49,180
Last modified:November 1, 1998 - v1
Checksum:iA578474DCBAAF7C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005960 mRNA. Translation: CAA06776.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005960 mRNA. Translation: CAA06776.1.

3D structure databases

ProteinModelPortaliO91262.
SMRiO91262. Positions 26-392.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Identification of a coronavirus hemagglutinin-esterase with a substrate specificity different from those of influenza C virus and bovine coronavirus."
    Klausegger A., Strobl B., Regl G., Kaser A., Luytjes W., Vlasak R.
    J. Virol. 73:3737-3743(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The sialate-4-O-acetylesterases of coronaviruses related to mouse hepatitis virus: a proposal to reorganize group 2 Coronaviridae."
    Wurzer W.J., Obojes K., Vlasak R.
    J. Gen. Virol. 83:395-402(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_CVPV
AccessioniPrimary (citable) accession number: O91262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.