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O91262

- HEMA_CVPV

UniProt

O91262 - HEMA_CVPV

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Puffinosis coronavirus (PV) (Puffinosis virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei45 – 451NucleophileBy similarity
    Active sitei342 – 3421Charge relay systemBy similarity
    Active sitei345 – 3451Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    OrganismiPuffinosis coronavirus (PV) (Puffinosis virus)
    Taxonomic identifieri76583 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiPuffinus puffinus (Manx shearwater) [TaxID: 48688]

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 439415Hemagglutinin-esterasePRO_0000037151Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 70By similarity
    Glycosylationi94 – 941N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi118 ↔ 167By similarity
    Glycosylationi152 – 1521N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi196 – 1961N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi202 ↔ 291By similarity
    Disulfide bondi210 ↔ 264By similarity
    Glycosylationi246 – 2461N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi322 ↔ 327By similarity
    Glycosylationi331 – 3311N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi337 – 3371N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi360 – 3601N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi363 ↔ 387By similarity
    Glycosylationi374 – 3741N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

    Structurei

    3D structure databases

    ProteinModelPortaliO91262.
    SMRiO91262. Positions 26-392.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 407383Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini429 – 43911IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei408 – 42821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni12 – 132121Esterase domain first partBy similarityAdd
    BLAST
    Regioni133 – 281149Receptor bindingBy similarityAdd
    BLAST
    Regioni282 – 395114Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O91262-1 [UniParc]FASTAAdd to Basket

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    MGSMCIAMAP RTLLLLIGCQ LALGFNEPLN VVSHLSDDWF LFGDSRSDCS    50
    YVENNGHPAF DWLDLPQELC HSGKISAKSG NSLFKSFHFT DWYNYTGEGD 100
    QVIFYEGVNF SPSHGFKCLA EGDNKRWMGN KARFYALVYK KMAYYRSLSF 150
    VNVSYSYGGK AKPTAICKDN TLTLNNPTFI SKESNYVDYY YESDANFTLE 200
    GCDEFIVPLC VFNGHSRGSS SDPANKYYMD SQMYYNMDTG VFYGFNSTLD 250
    VGNTAQNPGL DLTCIYYALT PGNYKAVSLE YLLTIPSKAI CLRKPKRFMP 300
    VQVVDSRWNN AKHSDNMTAV ACQTPYCLFR NTSSGYNGST HDVHHGGFHF 350
    RKLLSGLLYN VSCIAQQGAF FYNNVSSQWP VLGYGQCPTA ANIEFIAPVC 400
    LYDPLPVILL GVLLGIAVLI IVFLLFYFMT DSGVRLHEA 439
    Length:439
    Mass (Da):49,180
    Last modified:November 1, 1998 - v1
    Checksum:iA578474DCBAAF7C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005960 mRNA. Translation: CAA06776.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005960 mRNA. Translation: CAA06776.1 .

    3D structure databases

    ProteinModelPortali O91262.
    SMRi O91262. Positions 26-392.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a coronavirus hemagglutinin-esterase with a substrate specificity different from those of influenza C virus and bovine coronavirus."
      Klausegger A., Strobl B., Regl G., Kaser A., Luytjes W., Vlasak R.
      J. Virol. 73:3737-3743(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The sialate-4-O-acetylesterases of coronaviruses related to mouse hepatitis virus: a proposal to reorganize group 2 Coronaviridae."
      Wurzer W.J., Obojes K., Vlasak R.
      J. Gen. Virol. 83:395-402(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiHEMA_CVPV
    AccessioniPrimary (citable) accession number: O91262
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3