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O91254

- REP1_FBNY1

UniProt

O91254 - REP1_FBNY1

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Protein

Para-Rep C1

Gene

C1

Organism
Faba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Divalent metal cations, possibly magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Divalent metal cationSequence Analysis
Metal bindingi47 – 471Divalent metal cationSequence Analysis
Active sitei86 – 861For DNA cleavage activityBy similarity
Metal bindingi94 – 941Divalent metal cationSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi174 – 1829ATPBy similarity

GO - Molecular functioni

  1. ATPase activity, uncoupled Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. DNA binding Source: UniProtKB-KW
  4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. nucleotidyltransferase activity Source: UniProtKB-KW
  7. RNA binding Source: InterPro
  8. RNA helicase activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein-DNA covalent cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Para-Rep C1 (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
Short name:
Rep1
Alternative name(s):
ATP-dependent helicase C1
Replication-associated protein of non-essential DNA C1
Gene namesi
Name:C1
ORF Names:ORF1
OrganismiFaba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV)
Taxonomic identifieri291603 [NCBI]
Taxonomic lineageiVirusesssDNA virusesNanoviridaeNanovirus
Virus hostiCicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827]
Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]
ProteomesiUP000008665: Genome

Subcellular locationi

Host nucleus Curated

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Para-Rep C1PRO_0000378518Add
BLAST

Keywords - PTMi

Covalent protein-DNA linkage

Interactioni

Subunit structurei

Homooligomer (Potential). Rep binds to repeated DNA motifs (iterons) (By similarity).By similarityCurated

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi8 – 114RCR-1By similarity
Motifi47 – 526RCR-2By similarity
Motifi56 – 7722Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi86 – 894RCR-3By similarity

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Family and domain databases

InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamiPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O91254-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASKRWCFTL NYKTAVERES FISLFSRDEL NYFVCGDETA PTTNQKHLQG
60 70 80 90 100
YVSLKKMIRL GGLKKKFGYR AHWEIAKGDD FQNRDYCTKE TLISEIGAPV
110 120 130 140 150
KKGSNQRKIM DLYLQDPEEM QLKDPDTALR CNAKRLRIEY CSSFAVISLR
160 170 180 190 200
PWQSELHRVL MAEPDDRTII WVYGSDGGEG KSTFAKELIK YGWFYTAGGK
210 220 230 240 250
TQDILYMYAQ DPERNIAFDV PRCSSEMMNY QAMEMLKNRV FASTKYRPVD
260 270 280
LCVRKKVHLI VFANVSPDPT KISEDRIVII NC
Length:282
Mass (Da):32,677
Last modified:November 1, 1998 - v1
Checksum:iEFC5C1368AE261D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005968 Genomic DNA. Translation: CAA06791.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005968 Genomic DNA. Translation: CAA06791.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view ]
Pfami PF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Ten distinct circular ssDNA components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome."
    Katul L., Timchenko T., Gronenborn B., Vetten H.J.
    J. Gen. Virol. 79:3101-3109(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiREP1_FBNY1
AccessioniPrimary (citable) accession number: O91254
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

Caution

This protein is encoded by a subviral DNA that is not present in all isolates of the virus.Curated

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3