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O91254 (REP1_FBNY1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Para-Rep C1

Short name=Rep1
EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3
Alternative name(s):
ATP-dependent helicase C1
Replication-associated protein of non-essential DNA C1
Gene names
Name:C1
ORF Names:ORF1
OrganismFaba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV) [Complete proteome]
Taxonomic identifier291603 [NCBI]
Taxonomic lineageVirusesssDNA virusesNanoviridaeNanovirus
Virus hostCicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827]
Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese By similarity.

Subunit structure

Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Miscellaneous

The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

Sequence similarities

Belongs to the nanoviridea/circoviridae replication-associated protein family.

Caution

This protein is encoded by a subviral DNA that is not present in all isolates of the virus.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Para-Rep C1
PRO_0000378518

Regions

Nucleotide binding174 – 1829ATP By similarity
Motif8 – 114RCR-1 By similarity
Motif47 – 526RCR-2 By similarity
Motif56 – 7722Nuclear localization signal Potential
Motif86 – 894RCR-3 By similarity

Sites

Active site861For DNA cleavage activity By similarity
Metal binding381Divalent metal cation Potential
Metal binding471Divalent metal cation Potential
Metal binding941Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
O91254 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: EFC5C1368AE261D7

FASTA28232,677
        10         20         30         40         50         60 
MASKRWCFTL NYKTAVERES FISLFSRDEL NYFVCGDETA PTTNQKHLQG YVSLKKMIRL 

        70         80         90        100        110        120 
GGLKKKFGYR AHWEIAKGDD FQNRDYCTKE TLISEIGAPV KKGSNQRKIM DLYLQDPEEM 

       130        140        150        160        170        180 
QLKDPDTALR CNAKRLRIEY CSSFAVISLR PWQSELHRVL MAEPDDRTII WVYGSDGGEG 

       190        200        210        220        230        240 
KSTFAKELIK YGWFYTAGGK TQDILYMYAQ DPERNIAFDV PRCSSEMMNY QAMEMLKNRV 

       250        260        270        280 
FASTKYRPVD LCVRKKVHLI VFANVSPDPT KISEDRIVII NC 

« Hide

References

[1]"Ten distinct circular ssDNA components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome."
Katul L., Timchenko T., Gronenborn B., Vetten H.J.
J. Gen. Virol. 79:3101-3109(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ005968 Genomic DNA. Translation: CAA06791.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameREP1_FBNY1
AccessionPrimary (citable) accession number: O91254
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families