O89344 (L_HENDH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Hendra virus (isolate Horse/Autralia/Hendra/1994)

Taxonomic identifier

928303 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Henipavirus

Virus host

Pteropus alecto (Black flying fox) [TaxID: 9402]
Pteropus poliocephalus (Grey-headed flying fox) [TaxID: 9403]
Homo sapiens (Human) [TaxID: 9606]
Equus caballus (Horse) [TaxID: 9796]
Pteropus scapulatus (Little red flying fox) [TaxID: 94117]

Protein attributes

Sequence length	2244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxovirus L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2244	2244	RNA-directed RNA polymerase L		PRO_0000236010
Regions
	Domain	715 – 899	185	RdRp catalytic
	Nucleotide binding	1840 – 1849	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O89344 [UniParc]. Last modified June 1, 2003. Version 2. Checksum: F4C29F354CEA2E2E	FASTA	2,244	257,284
	10 20 30 40 50 60 MAHELSISDI IYPECHLDSP IVSGKLISAI EYAQLRHNQP NGDKRLTENI KINLQGKRRS 70 80 90 100 110 120 VYISRQSRLG NYIRDNIKNL KEFLHVSYPE CNKSLFSLKS PGMTSKLSNI MKKSFKAYNI 130 140 150 160 170 180 VSRKIIEMLQ NITRNLITQD QKDEVLGIYE QDRLSNIGKY MSQSQWYECF LFWFTIKTEM 190 200 210 220 230 240 RAVIKNSQKP KFRSDSCIIH MKDNNMEIVM NPNLVCIYKN DKDGKRCYYL TPEIVLMCCD 250 260 270 280 290 300 VLEGRMMIET SIKSDIKYQS LITRSNALWT FIDSLFPIMG NRIYNIVSMI EPLVLALLQL 310 320 330 340 350 360 KDEARILRGA FLHHCIKEIH QELIGCGFTD QKTRSIFIDD LLSVMNIDNI HLLAEFFSFF 370 380 390 400 410 420 RTFGHPILEA KTAADKVREH MLADKVLEYG PIMKAHAVFC GTIINGYRDR HRGAWPPLYL 430 440 450 460 470 480 PSHASKHIIR LKNSGESLTV DDCVKNWESF CGIQFDCFME LKLDSDLSMY MKDKALSPIK 490 500 510 520 530 540 EEWDSVYPRE VLNYTPPRST EPRRLVDVFV NDENFDPYNM LEYVLTGDYL TDEQFNVSYS 550 560 570 580 590 600 LKEKETKQAG RLFAKMTYKM RACQVIAEAL IASGVGKYFK ENGMVKDEHE LLKTLFQLSI 610 620 630 640 650 660 SSVPRGNSQG RDSEFSNNTE KSLISLKRTT GRLLNNEVPC RMNIMSALID KNQSDQKKHN 670 680 690 700 710 720 ILPNTRNRHK CDNTSQTFLD YHMEFSPYKS DRMDRTETSD FSKYDDGTGT KFDTVSAFLT 730 740 750 760 770 780 TDLKKFCLNW RYESMAIFAE RLDEIYGLPG FFNWMHKRLE KSVIYVADPN CPPDIGKHIN 790 800 810 820 830 840 LDDTPEDDIF IHSPKGGIEG YSQKTWTIAT IPFLFLSAYE TNTRIAAIVQ GDNESIAITQ 850 860 870 880 890 900 KVHPNLPYKV KKEICARQAQ LYFDRLRMNL RALGLNLKAT ETIISTHLFV YSKKIHYDGA 910 920 930 940 950 960 VLSQALKSMS RCCFWSETLV DETRSACSNI STTIAKAIEN GLSRNVGYCI NVLKVIQQLL 970 980 990 1000 1010 1020 ISTEFSINET LTADVTSPIS NNLDWLVTAS RIPAPIGGFN YLNLSRIFVR NIGDPVTASL 1030 1040 1050 1060 1070 1080 ADLKRMIEHD LMTDKVLQKV MNQEPGDASF LDWASDPYSG NLPDSQSITK TIKNITARTI 1090 1100 1110 1120 1130 1140 LRTSPNPMLK GLFHDKSFEE DLELATFLMD RRIILPRAAH EILDNSLTGA REEIAGLLDT 1150 1160 1170 1180 1190 1200 TKGLIRSGLK KSGIQPKLVS RLSNHDYNQF LILNRLLSNK KRNDLISPKT CSVDLAKALR 1210 1220 1230 1240 1250 1260 CHMWRDLALG RSIYGLEVPD ALEAMTGRYI TGSMECQLCD QGNTMYGWFF VPRDSQLDQV 1270 1280 1290 1300 1310 1320 NKEHSSIRVP YVGSSTDERS DIKLGNVKRP TRALRSAIEL QLFIPGHTEI LKKVGMRLGT 1330 1340 1350 1360 1370 1380 WLPRGVNIDI DVLKAITPVS TSNNLSHRLR DRSTQFKLPG SVLNRVSRYV NISNDNLDFR 1390 1400 1410 1420 1430 1440 VEGEKVDTNL IYQQTMLLGL SVLEGKFRLR TETDDYNGIY HLHVRDNCCV KEVADIGGVN 1450 1460 1470 1480 1490 1500 AELPVPEYTE VENNRLIYDP DPVSEIDCDR LSKQESKARE LDFPLWSTEE LHDVLAKTVA 1510 1520 1530 1540 1550 1560 QTVLEIITKA DKDVLKQHLA IDSDDSINSL ITEFLMVDPE LFALYLGQSI SVKWAFEIHH 1570 1580 1590 1600 1610 1620 RRPHGRHTMV DLLSDLISNT SKHTYKVLSN ALSHPRVFKR FVNCGLLLPT QGPYLHQQDF 1630 1640 1650 1660 1670 1680 EKLSQNLLIT SYMNYLMNWC DFKKFPFLIA EQDEAVVELR EDIITSKHLC MIIDLYANHH 1690 1700 1710 1720 1730 1740 KPPWIIDLNP QEKICVLRDF ISKCRHTDVS SRSWNITDLD FMVFYASLTY LRRGIIKQLR 1750 1760 1770 1780 1790 1800 IRQVTEVIDT TTMLRDNILV ENPPIKTGVL DIRGCIIYNL EEILSMNTKS TSRKVFNLGS 1810 1820 1830 1840 1850 1860 KLSVENHKYR RIGLNSSSCY KALNLSPLIQ RYLPAGSQRL FVGEGSGSMM LLYQQTLGCS 1870 1880 1890 1900 1910 1920 ISFYNSGIDG DYIPGQRELR LFPSEYSIAE DDPSQSDKLK GLVVPLFNGR PETTWIGNLD 1930 1940 1950 1960 1970 1980 SYEYIINRTA GRNIGLVHSD MESGIDKQVE EIMIEHSHLI SIAINVMIED GVLVSKIAFA 1990 2000 2010 2020 2030 2040 PGFPISRLLN MYRSYFGLVL VCFPVYSNPE STEVYLICLQ KTIKTIIPPQ KVLDHSYLSD 2050 2060 2070 2080 2090 2100 EINDQGITSV IFKIKNIQSK QFHEDLVKHY QVEQPFFVPS HITCDEKLLM QAGLKMNGPE 2110 2120 2130 2140 2150 2160 ILKNEVGYDI GSDINTLRST IIILLNEAMN YFDDERSPSH HLEPFPVLEK TRVKTIMGRV 2170 2180 2190 2200 2210 2220 TRKVTVYSLI KLKETKSPEL YNIKNYIRRK VLILDFRSHT MIKLLPKGMK ERREKSGFKE 2230 2240 IWIFDLSNRE VKIWWKIIGY LSLV

References

[1]

"The exceptionally large genome of Hendra virus: support for creation of a new genus within the family Paramyxoviridae." Wang L.-F., Yu M., Hansson E., Pritchard L.I., Shiell B., Michalski W.P., Eaton B.T. J. Virol. 74:9972-9979(2000) [PubMed: 11024125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017149 Genomic RNA. Translation: AAC83194.2.
PIR	T08212.
RefSeq	NP_047113.2. NC_001906.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1446468.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR016269. RNA-dir_pol_paramyxovirus. IPR024352. RNA_pol_cap_MeTfrase. [Graphical view]
Pfam	PF12803. G-7-MTase. 1 hit. PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_HENDH

Accession

Primary (citable) accession number: O89344

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program