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Protein

Vesicle transport through interaction with t-SNAREs homolog 1A

Gene

Vti1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be concerned with increased secretion of cytokines associated with cellular senescence.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.
R-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2
Gene namesi
Name:Vti1a
Synonyms:Vti1, Vti1l2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1855699. Vti1a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 192192CytoplasmicSequence analysisAdd
BLAST
Transmembranei193 – 21321Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini214 – 2174VesicularSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Vesicle transport through interaction with t-SNAREs homolog 1APRO_0000218226Add
BLAST

Proteomic databases

EPDiO89116.
MaxQBiO89116.
PaxDbiO89116.
PRIDEiO89116.

PTM databases

iPTMnetiO89116.
PhosphoSiteiO89116.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiO89116.
GenevisibleiO89116. MM.

Interactioni

Subunit structurei

Interacts with distinct SNARE complexes that contain either STX5 or STX6. Interacts with NAPA and, to a lesser extent, with NAPG. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207331. 2 interactions.
IntActiO89116. 2 interactions.
MINTiMINT-4140161.
STRINGi10090.ENSMUSP00000093644.

Structurei

Secondary structure

1
217
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 2517Combined sources
Helixi26 – 283Combined sources
Turni31 – 333Combined sources
Helixi34 – 5926Combined sources
Turni64 – 663Combined sources
Helixi67 – 8721Combined sources
Helixi89 – 924Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCSNMR-A6-94[»]
ProteinModelPortaliO89116.
SMRiO89116. Positions 8-94, 113-190.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO89116.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili31 – 9262Sequence analysisAdd
BLAST
Coiled coili112 – 17867Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the VTI1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1666. Eukaryota.
ENOG4111J90. LUCA.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG104027.
InParanoidiO89116.
KOiK08493.
OMAiGRVESHR.
OrthoDBiEOG7MKW77.
PhylomeDBiO89116.
TreeFamiTF312874.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.

Sequencei

Sequence statusi: Complete.

O89116-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDFEGYEQ DFAVLTAEIT SKIARVPRLP PDEKKQMVAN VEKQLEEARE
60 70 80 90 100
LLEQMDLEVR EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR
110 120 130 140 150
NELLGDAGNS SENQRAHLLD NTERLERSSR RLEAGYQIAV ETEQIGQEML
160 170 180 190 200
ENLSHDREKI QRARDRLRDA DANLGKSSRI LTGMLRRIIQ NRILLVILGI
210
IVVIAILTAI AFFVKGH
Length:217
Mass (Da):24,986
Last modified:November 1, 1998 - v1
Checksum:i3FD7B7A5A16E3522
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035823 mRNA. Translation: AAC32049.1.
AF035209 mRNA. Translation: AAC23482.1.
AK004751 mRNA. Translation: BAB23532.1.
AK028646 mRNA. Translation: BAC26046.1.
BC019386 mRNA. Translation: AAH19386.1.
CCDSiCCDS29910.1.
RefSeqiNP_001280615.1. NM_001293686.1.
NP_058558.1. NM_016862.4.
UniGeneiMm.258637.
Mm.451075.
Mm.451214.

Genome annotation databases

EnsembliENSMUST00000095950; ENSMUSP00000093644; ENSMUSG00000024983.
GeneIDi53611.
KEGGimmu:53611.
UCSCiuc008hxw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035823 mRNA. Translation: AAC32049.1.
AF035209 mRNA. Translation: AAC23482.1.
AK004751 mRNA. Translation: BAB23532.1.
AK028646 mRNA. Translation: BAC26046.1.
BC019386 mRNA. Translation: AAH19386.1.
CCDSiCCDS29910.1.
RefSeqiNP_001280615.1. NM_001293686.1.
NP_058558.1. NM_016862.4.
UniGeneiMm.258637.
Mm.451075.
Mm.451214.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VCSNMR-A6-94[»]
ProteinModelPortaliO89116.
SMRiO89116. Positions 8-94, 113-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207331. 2 interactions.
IntActiO89116. 2 interactions.
MINTiMINT-4140161.
STRINGi10090.ENSMUSP00000093644.

PTM databases

iPTMnetiO89116.
PhosphoSiteiO89116.

Proteomic databases

EPDiO89116.
MaxQBiO89116.
PaxDbiO89116.
PRIDEiO89116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095950; ENSMUSP00000093644; ENSMUSG00000024983.
GeneIDi53611.
KEGGimmu:53611.
UCSCiuc008hxw.2. mouse.

Organism-specific databases

CTDi143187.
MGIiMGI:1855699. Vti1a.

Phylogenomic databases

eggNOGiKOG1666. Eukaryota.
ENOG4111J90. LUCA.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG104027.
InParanoidiO89116.
KOiK08493.
OMAiGRVESHR.
OrthoDBiEOG7MKW77.
PhylomeDBiO89116.
TreeFamiTF312874.

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.
R-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

ChiTaRSiVti1a. mouse.
EvolutionaryTraceiO89116.
PROiO89116.
SOURCEiSearch...

Gene expression databases

BgeeiO89116.
GenevisibleiO89116. MM.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway."
    Xu Y., Wong S.H., Tang B.L., Subramaniam V.N., Zhang T., Hong W.
    J. Biol. Chem. 273:21783-21789(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH NAPA AND NAPG, IDENTIFICATION IN SNARE COMPLEXES WITH STX5 OR STX6, TISSUE SPECIFICITY.
  2. "Seven novel mammalian SNARE proteins localize to distinct membrane compartments."
    Advani R.J., Bae H.-R., Bock J.B., Chao D.S., Doung Y.-C., Prekeris R., Yoo J.-S., Scheller R.H.
    J. Biol. Chem. 273:10317-10324(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung and Skin.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  5. "A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels."
    Flowerdew S.E., Burgoyne R.D.
    Biochem. J. 418:529-540(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Lung, Spleen and Testis.
  7. "Solution structure of RSGI RUH-009, an N-terminal domain of VTI1A [Mus musculus]."
    RIKEN structural genomics initiative (RSGI)
    Submitted (MAY-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 3-94.

Entry informationi

Entry nameiVTI1A_MOUSE
AccessioniPrimary (citable) accession number: O89116
Secondary accession number(s): Q545P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.