O89116 (VTI1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1A

Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 2
Vti1-rp2

Gene names

Name:	Vti1a
Synonyms:	Vti1, Vti1l2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be concerned with increased secretion of cytokines associated with cellular senescence. Ref.5
Subunit structure	Interacts with distinct SNARE complexes that contain either STX5 or STX6. Interacts with NAPA and, to a lesser extent, with NAPG. Also identified in a complex containing STX13 and VAMP4 By similarity. Ref.1
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein By similarity.
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the VTI1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde transport, endosome to Golgi Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 217

217

Vesicle transport through interaction with t-SNAREs homolog 1A

PRO_0000218226

Regions

Topological domain

1 – 192

192

Cytoplasmic Potential

Transmembrane

193 – 213

21

Helical; Anchor for type IV membrane protein; Potential

Topological domain

214 – 217

4

Vesicular Potential

Coiled coil

31 – 92

62

Potential

Coiled coil

112 – 178

67

Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

217

Helix Strand Turn

Details...

Helix

9 – 25

17

Helix

26 – 28

3

Turn

31 – 33

3

Helix

34 – 59

26

Turn

64 – 66

3

Helix

67 – 87

21

Helix

89 – 92

4

Sequences

	Sequence		Length	Mass (Da)
	O89116 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 3FD7B7A5A16E3522	FASTA	217	24,986
	10 20 30 40 50 60 MSSDFEGYEQ DFAVLTAEIT SKIARVPRLP PDEKKQMVAN VEKQLEEARE LLEQMDLEVR 70 80 90 100 110 120 EIPPQSRGMY SNRMRSYKQE MGKLETDFKR SRIAYSDEVR NELLGDAGNS SENQRAHLLD 130 140 150 160 170 180 NTERLERSSR RLEAGYQIAV ETEQIGQEML ENLSHDREKI QRARDRLRDA DANLGKSSRI 190 200 210 LTGMLRRIIQ NRILLVILGI IVVIAILTAI AFFVKGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway." Xu Y., Wong S.H., Tang B.L., Subramaniam V.N., Zhang T., Hong W. J. Biol. Chem. 273:21783-21789(1998) [PubMed: 9705316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH NAPA AND NAPG, IDENTIFICATION IN SNARE COMPLEXES WITH STX5 OR STX6, TISSUE SPECIFICITY.
[2]	"Seven novel mammalian SNARE proteins localize to distinct membrane compartments." Advani R.J., Bae H.-R., Bock J.B., Chao D.S., Doung Y.-C., Prekeris R., Yoo J.-S., Scheller R.H. J. Biol. Chem. 273:10317-10324(1998) [PubMed: 9553086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung and Skin.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[5]	"A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels." Flowerdew S.E., Burgoyne R.D. Biochem. J. 418:529-540(2009) [PubMed: 19138172] [Abstract] Cited for: FUNCTION.
[6]	"Solution structure of RSGI RUH-009, an N-terminal domain of VTI1A [Mus musculus]." RIKEN structural genomics initiative (RSGI) Submitted (MAY-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 3-94.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF035823 mRNA. Translation: AAC32049.1.
AF035209 mRNA. Translation: AAC23482.1.
AK004751 mRNA. Translation: BAB23532.1.
AK028646 mRNA. Translation: BAC26046.1.
BC019386 mRNA. Translation: AAH19386.1.

IPI

IPI00131540.

RefSeq

NP_058558.1. NM_016862.3.

UniGene

Mm.258637.
Mm.451214.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VCS	NMR	-	A	6-94	[»]

ProteinModelPortal

O89116.

SMR

O89116. Positions 8-94, 113-190.

ModBase

Search...

Protein-protein interaction databases

IntAct

O89116. 1 interaction.

STRING

O89116.

PTM databases

PhosphoSite

O89116.

Proteomic databases

PRIDE

O89116.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000095950; ENSMUSP00000093644; ENSMUSG00000024983.

GeneID

53611.

KEGG

mmu:53611.

UCSC

uc008hxx.1. mouse.

Organism-specific databases

CTD

143187.

MGI

MGI:1855699. Vti1a.

Phylogenomic databases

eggNOG

roNOG15861.

GeneTree

ENSGT00530000063466.

HOVERGEN

HBG104027.

OrthoDB

EOG4V6ZHV.

PhylomeDB

O89116.

Gene expression databases

ArrayExpress

O89116.

Bgee

O89116.

Genevestigator

O89116.

GermOnline

ENSMUSG00000024983. Mus musculus.

Family and domain databases

InterPro

IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]

KO

K08493.

Pfam

PF05008. V-SNARE. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

ProtoNet

Search...

Other

NextBio

310323.

SOURCE

Search...

Entry information

Entry name

VTI1A_MOUSE

Accession

Primary (citable) accession number: O89116
Secondary accession number(s): Q545P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program