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Protein

Immediate early response gene 5 protein

Gene

Ier5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role as a transcription factor. Mediates positive transcriptional regulation of several chaperone gene during the heat shock response in a HSF1-dependent manner. Mediates negative transcriptional regulation of CDC25B expression. Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the regulation of cell proliferation and resistance to thermal stress. Involved in the cell cycle checkpoint and survival in response to ionizing radiation. Associates with chromatin to the CDC25B promoter.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processStress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Immediate early response gene 5 protein
Gene namesi
Name:Ier5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1337072. Ier5.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Predominantly cytoplasmic. Translocated in the nucleus during heat shock.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001904391 – 308Immediate early response gene 5 proteinAdd BLAST308

Proteomic databases

EPDiO89113.
PaxDbiO89113.
PRIDEiO89113.

PTM databases

PhosphoSitePlusiO89113.

Expressioni

Inductioni

By serum and growth factors.1 Publication

Gene expression databases

BgeeiENSMUSG00000056708.
CleanExiMM_IER5.
ExpressionAtlasiO89113. baseline and differential.
GenevisibleiO89113. MM.

Interactioni

Subunit structurei

Monomer. Homodimer. Associates with the catalytic subunit of protein phosphatase PP2A. Interacts (via N- and C-terminal regions) with PPP2R2B. Interacts with PPP2R2A, PPP2R2C and PPP2R2D. Interacts (via N-terminus) with RPS6KB1. Interacts (via central region) with HSF1; this interaction promotes PPP2CA-induced HSF1 dephosphorylation, leading to enhanced HSF1 transcriptional activity.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056948.

Structurei

3D structure databases

ProteinModelPortaliO89113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IER family.Curated

Phylogenomic databases

eggNOGiENOG410J10V. Eukaryota.
ENOG4111NZJ. LUCA.
GeneTreeiENSGT00730000111126.
HOGENOMiHOG000112999.
HOVERGENiHBG105469.
InParanoidiO89113.
OMAiDMNPWST.
OrthoDBiEOG091G0HLK.
PhylomeDBiO89113.
TreeFamiTF331376.

Family and domain databases

InterProiView protein in InterPro
IPR008653. IER.
PANTHERiPTHR15895. PTHR15895. 1 hit.
PfamiView protein in Pfam
PF05760. IER. 2 hits.

Sequencei

Sequence statusi: Complete.

O89113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFKLEAHRI VSISLGKIYN SRVQRGGIKL HKNLLVSLVL RSARQVYLSD
60 70 80 90 100
PCPGLYLAGP AGTPAVPPPQ QPGEPVAGPP SGWGEPPPPV ARAAWPEPEP
110 120 130 140 150
QPQPQPQRPS VCHTPGAGSS EPVAAVAGSG EALRGGEEDS AAAAWGRVER
160 170 180 190 200
PRAASSGGGS DACPEGPRAV RRPCGCPPAV EERSSEDGSP APPAPCPRKR
210 220 230 240 250
GAAGVGGGRA SCSAPGSTPL KKPRRNSEEQ PVTGEDDTDE EMETGNVANL
260 270 280 290 300
ISIFGSSFSG LLRKSPAGGR EEEEAEEGGP EAAEPGQICC DKPVLRDMSP

WSTAIVAF
Length:308
Mass (Da):31,784
Last modified:November 1, 1998 - v1
Checksum:iF9484E63F69FC02B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079527 mRNA. Translation: AAC31197.1.
AF079528 Genomic DNA. Translation: AAC28389.1.
BC037147 mRNA. Translation: AAH37147.1.
BC051986 mRNA. Translation: AAH51986.1.
CCDSiCCDS15382.1.
RefSeqiNP_034630.1. NM_010500.2.
UniGeneiMm.12246.

Genome annotation databases

EnsembliENSMUST00000055322; ENSMUSP00000056948; ENSMUSG00000056708.
GeneIDi15939.
KEGGimmu:15939.
UCSCiuc007dax.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079527 mRNA. Translation: AAC31197.1.
AF079528 Genomic DNA. Translation: AAC28389.1.
BC037147 mRNA. Translation: AAH37147.1.
BC051986 mRNA. Translation: AAH51986.1.
CCDSiCCDS15382.1.
RefSeqiNP_034630.1. NM_010500.2.
UniGeneiMm.12246.

3D structure databases

ProteinModelPortaliO89113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056948.

PTM databases

PhosphoSitePlusiO89113.

Proteomic databases

EPDiO89113.
PaxDbiO89113.
PRIDEiO89113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055322; ENSMUSP00000056948; ENSMUSG00000056708.
GeneIDi15939.
KEGGimmu:15939.
UCSCiuc007dax.2. mouse.

Organism-specific databases

CTDi51278.
MGIiMGI:1337072. Ier5.

Phylogenomic databases

eggNOGiENOG410J10V. Eukaryota.
ENOG4111NZJ. LUCA.
GeneTreeiENSGT00730000111126.
HOGENOMiHOG000112999.
HOVERGENiHBG105469.
InParanoidiO89113.
OMAiDMNPWST.
OrthoDBiEOG091G0HLK.
PhylomeDBiO89113.
TreeFamiTF331376.

Miscellaneous databases

ChiTaRSiIer5. mouse.
PROiPR:O89113.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056708.
CleanExiMM_IER5.
ExpressionAtlasiO89113. baseline and differential.
GenevisibleiO89113. MM.

Family and domain databases

InterProiView protein in InterPro
IPR008653. IER.
PANTHERiPTHR15895. PTHR15895. 1 hit.
PfamiView protein in Pfam
PF05760. IER. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiIER5_MOUSE
AccessioniPrimary (citable) accession number: O89113
Secondary accession number(s): Q8CGI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1998
Last modified: April 12, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.