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Protein

Intermediate conductance calcium-activated potassium channel protein 4

Gene

Kcnn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.2 Publications

GO - Molecular functioni

  • calcium-activated potassium channel activity Source: MGI
  • potassium channel activity Source: MGI
  • protein phosphatase binding Source: MGI
  • small conductance calcium-activated potassium channel activity Source: InterPro

GO - Biological processi

  • calcium ion transport Source: MGI
  • cell volume homeostasis Source: MGI
  • immune system process Source: UniProtKB-KW
  • phospholipid translocation Source: MGI
  • positive regulation of protein secretion Source: MGI
  • positive regulation of T cell receptor signaling pathway Source: MGI
  • saliva secretion Source: MGI
  • stabilization of membrane potential Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Immunity, Ion transport, Transport

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-1296052. Ca2+ activated K+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Intermediate conductance calcium-activated potassium channel protein 4
Short name:
SK4
Short name:
SKCa 4
Short name:
SKCa4
Alternative name(s):
IK1
KCa3.1
KCa4
Gene namesi
Name:Kcnn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1277957. Kcnn4.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50Helical; Name=Segment S1Sequence analysisAdd BLAST21
Transmembranei59 – 79Helical; Name=Segment S2Sequence analysisAdd BLAST21
Transmembranei105 – 121Helical; Name=Segment S3Sequence analysisAdd BLAST17
Transmembranei141 – 161Helical; Name=Segment S4Sequence analysisAdd BLAST21
Transmembranei205 – 225Helical; Name=Segment S5Sequence analysisAdd BLAST21
Intramembranei239 – 259Pore-forming; Name=Segment H5Sequence analysisAdd BLAST21
Transmembranei263 – 283Helical; Name=Segment S6Sequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: MGI
  • plasma membrane Source: UniProtKB
  • vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001550181 – 425Intermediate conductance calcium-activated potassium channel protein 4Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei356PhosphohistidineBy similarity1

Post-translational modificationi

Phosphorylation at His-356 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO89109.
MaxQBiO89109.
PaxDbiO89109.
PRIDEiO89109.

PTM databases

iPTMnetiO89109.
PhosphoSitePlusiO89109.

Expressioni

Gene expression databases

BgeeiENSMUSG00000054342.
ExpressionAtlasiO89109. baseline and differential.
GenevisibleiO89109. MM.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins (Probable). Interacts with MTMR6 (By similarity).By similarityCurated

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133065.

Structurei

3D structure databases

ProteinModelPortaliO89109.
SMRiO89109.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni284 – 345Calmodulin-bindingBy similarityAdd BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi413 – 416Poly-Gln4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3684. Eukaryota.
ENOG410XT9D. LUCA.
GeneTreeiENSGT00500000044784.
HOGENOMiHOG000276908.
HOVERGENiHBG052241.
InParanoidiO89109.
KOiK04945.
OMAiNINFTFR.
OrthoDBiEOG091G09X0.
PhylomeDBiO89109.
TreeFamiTF315015.

Family and domain databases

InterProiIPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
IPR013099. K_chnl_dom.
[Graphical view]
PANTHERiPTHR10153. PTHR10153. 1 hit.
PfamiPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
SMARTiSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMiSSF81327. SSF81327. 1 hit.

Sequencei

Sequence statusi: Complete.

O89109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGELVTGLG ALRRRKRLLE QEKRVAGWAL VLAGTGIGLM VLHAEMLWFL
60 70 80 90 100
GCKWVLYLLL VKCLITLSTA FLLCLIVVFH AKEVQLFMTD NGLRDWRVAL
110 120 130 140 150
TRRQVAQILL ELLVCGVHPV PLRSPHCALA GEATDAQPWP GFLGEGEALL
160 170 180 190 200
SLAMLLRLYL VPRAVLLRSG VLLNASYRSI GALNQVRFRH WFVAKLYMNT
210 220 230 240 250
HPGRLLLGLT LGLWLTTAWV LSVAERQAVN ATGHLTDTLW LIPITFLTIG
260 270 280 290 300
YGDVVPGTMW GKIVCLCTGV MGVCCTALLV AVVARKLEFN KAEKHVHNFM
310 320 330 340 350
MDIHYAKEMK ESAARLLQEA WMYYKHTRRK DSRAARRHQR KMLAAIHTFR
360 370 380 390 400
QVRLKHRKLR EQVNSMVDIS KMHMILCDLQ LGLSSSHRAL EKRIDGLAGK
410 420
LDALTELLGT ALQQQQLPEP SQEAT
Length:425
Mass (Da):47,784
Last modified:November 1, 1998 - v1
Checksum:iDCD2FC86BDD69A73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181G → E in BAB27283 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042487 mRNA. Translation: AAC32051.1.
AF072884 mRNA. Translation: AAC32829.1.
AK010943 mRNA. Translation: BAB27283.1.
AK155910 mRNA. Translation: BAE33499.1.
BC010274 mRNA. Translation: AAH10274.1.
CCDSiCCDS20948.1.
RefSeqiNP_001156982.1. NM_001163510.1.
NP_032459.3. NM_008433.4.
XP_006539626.1. XM_006539563.3.
UniGeneiMm.9911.

Genome annotation databases

EnsembliENSMUST00000171904; ENSMUSP00000133065; ENSMUSG00000054342.
ENSMUST00000205428; ENSMUSP00000146012; ENSMUSG00000054342.
GeneIDi16534.
KEGGimmu:16534.
UCSCiuc009fpk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042487 mRNA. Translation: AAC32051.1.
AF072884 mRNA. Translation: AAC32829.1.
AK010943 mRNA. Translation: BAB27283.1.
AK155910 mRNA. Translation: BAE33499.1.
BC010274 mRNA. Translation: AAH10274.1.
CCDSiCCDS20948.1.
RefSeqiNP_001156982.1. NM_001163510.1.
NP_032459.3. NM_008433.4.
XP_006539626.1. XM_006539563.3.
UniGeneiMm.9911.

3D structure databases

ProteinModelPortaliO89109.
SMRiO89109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133065.

PTM databases

iPTMnetiO89109.
PhosphoSitePlusiO89109.

Proteomic databases

EPDiO89109.
MaxQBiO89109.
PaxDbiO89109.
PRIDEiO89109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000171904; ENSMUSP00000133065; ENSMUSG00000054342.
ENSMUST00000205428; ENSMUSP00000146012; ENSMUSG00000054342.
GeneIDi16534.
KEGGimmu:16534.
UCSCiuc009fpk.2. mouse.

Organism-specific databases

CTDi3783.
MGIiMGI:1277957. Kcnn4.

Phylogenomic databases

eggNOGiKOG3684. Eukaryota.
ENOG410XT9D. LUCA.
GeneTreeiENSGT00500000044784.
HOGENOMiHOG000276908.
HOVERGENiHBG052241.
InParanoidiO89109.
KOiK04945.
OMAiNINFTFR.
OrthoDBiEOG091G09X0.
PhylomeDBiO89109.
TreeFamiTF315015.

Enzyme and pathway databases

ReactomeiR-MMU-1296052. Ca2+ activated K+ channels.

Miscellaneous databases

ChiTaRSiKcnn4. mouse.
PROiO89109.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054342.
ExpressionAtlasiO89109. baseline and differential.
GenevisibleiO89109. MM.

Family and domain databases

InterProiIPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
IPR013099. K_chnl_dom.
[Graphical view]
PANTHERiPTHR10153. PTHR10153. 1 hit.
PfamiPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
SMARTiSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMiSSF81327. SSF81327. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNN4_MOUSE
AccessioniPrimary (citable) accession number: O89109
Secondary accession number(s): Q3U1J8, Q9CY39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.