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Protein

Intermediate conductance calcium-activated potassium channel protein 4

Gene

Kcnn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.2 Publications

GO - Molecular functioni

GO - Biological processi

  • calcium ion transport Source: MGI
  • cell volume homeostasis Source: MGI
  • immune system process Source: UniProtKB-KW
  • phospholipid translocation Source: MGI
  • positive regulation of protein secretion Source: MGI
  • positive regulation of T cell receptor signaling pathway Source: MGI
  • potassium ion export Source: Ensembl
  • saliva secretion Source: MGI
  • stabilization of membrane potential Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Immunity, Ion transport, Transport

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-1296052. Ca2+ activated K+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Intermediate conductance calcium-activated potassium channel protein 4
Short name:
SK4
Short name:
SKCa 4
Short name:
SKCa4
Alternative name(s):
IK1
KCa3.1
KCa4
Gene namesi
Name:Kcnn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1277957. Kcnn4.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 5021Helical; Name=Segment S1Sequence analysisAdd
BLAST
Transmembranei59 – 7921Helical; Name=Segment S2Sequence analysisAdd
BLAST
Transmembranei105 – 12117Helical; Name=Segment S3Sequence analysisAdd
BLAST
Transmembranei141 – 16121Helical; Name=Segment S4Sequence analysisAdd
BLAST
Transmembranei205 – 22521Helical; Name=Segment S5Sequence analysisAdd
BLAST
Intramembranei239 – 25921Pore-forming; Name=Segment H5Sequence analysisAdd
BLAST
Transmembranei263 – 28321Helical; Name=Segment S6Sequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Intermediate conductance calcium-activated potassium channel protein 4PRO_0000155018Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei356 – 3561PhosphohistidineBy similarity

Post-translational modificationi

Phosphorylation at His-356 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO89109.
MaxQBiO89109.
PaxDbiO89109.
PRIDEiO89109.

PTM databases

PhosphoSiteiO89109.

Expressioni

Gene expression databases

BgeeiO89109.
GenevisibleiO89109. MM.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins (Probable). Interacts with MTMR6 (By similarity).By similarityCurated

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133065.

Structurei

3D structure databases

ProteinModelPortaliO89109.
SMRiO89109. Positions 285-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni284 – 34562Calmodulin-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi413 – 4164Poly-Gln

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3684. Eukaryota.
ENOG410XT9D. LUCA.
GeneTreeiENSGT00500000044784.
HOGENOMiHOG000276908.
HOVERGENiHBG052241.
InParanoidiO89109.
KOiK04945.
OMAiLQEAWMY.
OrthoDBiEOG7KSX8J.
PhylomeDBiO89109.
TreeFamiTF315015.

Family and domain databases

InterProiIPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
IPR013099. K_chnl_dom.
[Graphical view]
PANTHERiPTHR10153. PTHR10153. 1 hit.
PfamiPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
SMARTiSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMiSSF81327. SSF81327. 1 hit.

Sequencei

Sequence statusi: Complete.

O89109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGELVTGLG ALRRRKRLLE QEKRVAGWAL VLAGTGIGLM VLHAEMLWFL
60 70 80 90 100
GCKWVLYLLL VKCLITLSTA FLLCLIVVFH AKEVQLFMTD NGLRDWRVAL
110 120 130 140 150
TRRQVAQILL ELLVCGVHPV PLRSPHCALA GEATDAQPWP GFLGEGEALL
160 170 180 190 200
SLAMLLRLYL VPRAVLLRSG VLLNASYRSI GALNQVRFRH WFVAKLYMNT
210 220 230 240 250
HPGRLLLGLT LGLWLTTAWV LSVAERQAVN ATGHLTDTLW LIPITFLTIG
260 270 280 290 300
YGDVVPGTMW GKIVCLCTGV MGVCCTALLV AVVARKLEFN KAEKHVHNFM
310 320 330 340 350
MDIHYAKEMK ESAARLLQEA WMYYKHTRRK DSRAARRHQR KMLAAIHTFR
360 370 380 390 400
QVRLKHRKLR EQVNSMVDIS KMHMILCDLQ LGLSSSHRAL EKRIDGLAGK
410 420
LDALTELLGT ALQQQQLPEP SQEAT
Length:425
Mass (Da):47,784
Last modified:November 1, 1998 - v1
Checksum:iDCD2FC86BDD69A73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811G → E in BAB27283 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042487 mRNA. Translation: AAC32051.1.
AF072884 mRNA. Translation: AAC32829.1.
AK010943 mRNA. Translation: BAB27283.1.
AK155910 mRNA. Translation: BAE33499.1.
BC010274 mRNA. Translation: AAH10274.1.
CCDSiCCDS20948.1.
RefSeqiNP_001156982.1. NM_001163510.1.
NP_032459.3. NM_008433.4.
XP_006539626.1. XM_006539563.2.
UniGeneiMm.9911.

Genome annotation databases

EnsembliENSMUST00000171904; ENSMUSP00000133065; ENSMUSG00000054342.
ENSMUST00000205428; ENSMUSP00000146012; ENSMUSG00000054342.
GeneIDi16534.
KEGGimmu:16534.
UCSCiuc009fpk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042487 mRNA. Translation: AAC32051.1.
AF072884 mRNA. Translation: AAC32829.1.
AK010943 mRNA. Translation: BAB27283.1.
AK155910 mRNA. Translation: BAE33499.1.
BC010274 mRNA. Translation: AAH10274.1.
CCDSiCCDS20948.1.
RefSeqiNP_001156982.1. NM_001163510.1.
NP_032459.3. NM_008433.4.
XP_006539626.1. XM_006539563.2.
UniGeneiMm.9911.

3D structure databases

ProteinModelPortaliO89109.
SMRiO89109. Positions 285-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133065.

PTM databases

PhosphoSiteiO89109.

Proteomic databases

EPDiO89109.
MaxQBiO89109.
PaxDbiO89109.
PRIDEiO89109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000171904; ENSMUSP00000133065; ENSMUSG00000054342.
ENSMUST00000205428; ENSMUSP00000146012; ENSMUSG00000054342.
GeneIDi16534.
KEGGimmu:16534.
UCSCiuc009fpk.2. mouse.

Organism-specific databases

CTDi3783.
MGIiMGI:1277957. Kcnn4.

Phylogenomic databases

eggNOGiKOG3684. Eukaryota.
ENOG410XT9D. LUCA.
GeneTreeiENSGT00500000044784.
HOGENOMiHOG000276908.
HOVERGENiHBG052241.
InParanoidiO89109.
KOiK04945.
OMAiLQEAWMY.
OrthoDBiEOG7KSX8J.
PhylomeDBiO89109.
TreeFamiTF315015.

Enzyme and pathway databases

ReactomeiR-MMU-1296052. Ca2+ activated K+ channels.

Miscellaneous databases

ChiTaRSiKcnn4. mouse.
NextBioi289957.
PROiO89109.
SOURCEiSearch...

Gene expression databases

BgeeiO89109.
GenevisibleiO89109. MM.

Family and domain databases

InterProiIPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
IPR013099. K_chnl_dom.
[Graphical view]
PANTHERiPTHR10153. PTHR10153. 1 hit.
PfamiPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
SMARTiSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMiSSF81327. SSF81327. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation."
    Vandorpe D.H., Shmukler B.E., Jiang L., Lim B., Maylie J., Adelman J.P., de Franceschi L., Cappellini M.D., Brugnara C., Alper S.L.
    J. Biol. Chem. 273:21542-21553(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: CD-1.
  2. "Murine intermediate conductance calcium-activated potassium channel (mKCa4, KCNN4) complete cds."
    Logsdon N.J., Aiyar J.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  5. "Nucleoside diphosphate kinase B knock-out mice have impaired activation of the K+ channel KCa3.1, resulting in defective T cell activation."
    Di L., Srivastava S., Zhdanova O., Sun Y., Li Z., Skolnik E.Y.
    J. Biol. Chem. 285:38765-38771(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiKCNN4_MOUSE
AccessioniPrimary (citable) accession number: O89109
Secondary accession number(s): Q3U1J8, Q9CY39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 1998
Last modified: March 16, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.