O89109 (KCNN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intermediate conductance calcium-activated potassium channel protein 4
Short name=SK4
Short name=SKCa 4
Short name=SKCa4

Alternative name(s):
IK1
KCa3.1
KCa4

Gene names

Name:

Kcnn4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. Ref.1 Ref.5
Subunit structure	Heterotetramer of potassium channel proteins Probable. Interacts with MTMR6 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylation at His-356 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel By similarity.
Sequence similarities	Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.

Ontologies

Keywords
Biological process	Immunity Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Calmodulin-binding
Molecular function	Ionic channel
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell volume homeostasis Inferred from mutant phenotype. Source: MGI phospholipid translocation Inferred from mutant phenotype. Source: MGI positive regulation of protein secretion Inferred from direct assay. Source: MGI saliva secretion Inferred from genetic interaction. Source: MGI
Cellular component	integral to plasma membrane Inferred by curator. Source: MGI
Molecular function	calcium-activated potassium channel activity Inferred from direct assay. Source: MGI calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Intermediate conductance calcium-activated potassium channel protein 4		PRO_0000155018
Regions
	Transmembrane	30 – 50	21	Helical; Name=Segment S1; Potential
	Transmembrane	59 – 79	21	Helical; Name=Segment S2; Potential
	Transmembrane	105 – 121	17	Helical; Name=Segment S3; Potential
	Transmembrane	141 – 161	21	Helical; Name=Segment S4; Potential
	Transmembrane	205 – 225	21	Helical; Name=Segment S5; Potential
	Intramembrane	239 – 259	21	Pore-forming; Name=Segment H5; Potential
	Transmembrane	263 – 283	21	Helical; Name=Segment S6; Potential
	Region	284 – 345	62	Calmodulin-binding By similarity
	Compositional bias	413 – 416	4	Poly-Gln
Amino acid modifications
	Modified residue	356	1	Phosphohistidine By similarity
Experimental info
	Sequence conflict	181	1	G → E in BAB27283. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O89109 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: DCD2FC86BDD69A73	FASTA	425	47,784
	10 20 30 40 50 60 MGGELVTGLG ALRRRKRLLE QEKRVAGWAL VLAGTGIGLM VLHAEMLWFL GCKWVLYLLL 70 80 90 100 110 120 VKCLITLSTA FLLCLIVVFH AKEVQLFMTD NGLRDWRVAL TRRQVAQILL ELLVCGVHPV 130 140 150 160 170 180 PLRSPHCALA GEATDAQPWP GFLGEGEALL SLAMLLRLYL VPRAVLLRSG VLLNASYRSI 190 200 210 220 230 240 GALNQVRFRH WFVAKLYMNT HPGRLLLGLT LGLWLTTAWV LSVAERQAVN ATGHLTDTLW 250 260 270 280 290 300 LIPITFLTIG YGDVVPGTMW GKIVCLCTGV MGVCCTALLV AVVARKLEFN KAEKHVHNFM 310 320 330 340 350 360 MDIHYAKEMK ESAARLLQEA WMYYKHTRRK DSRAARRHQR KMLAAIHTFR QVRLKHRKLR 370 380 390 400 410 420 EQVNSMVDIS KMHMILCDLQ LGLSSSHRAL EKRIDGLAGK LDALTELLGT ALQQQQLPEP SQEAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation." Vandorpe D.H., Shmukler B.E., Jiang L., Lim B., Maylie J., Adelman J.P., de Franceschi L., Cappellini M.D., Brugnara C., Alper S.L. J. Biol. Chem. 273:21542-21553(1998) [PubMed: 9705284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: CD-1.
[2]	"Murine intermediate conductance calcium-activated potassium channel (mKCa4, KCNN4) complete cds." Logsdon N.J., Aiyar J. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryonic liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[5]	"Nucleoside diphosphate kinase B knock-out mice have impaired activation of the K+ channel KCa3.1, resulting in defective T cell activation." Di L., Srivastava S., Zhdanova O., Sun Y., Li Z., Skolnik E.Y. J. Biol. Chem. 285:38765-38771(2010) [PubMed: 20884616] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042487 mRNA. Translation: AAC32051.1. AF072884 mRNA. Translation: AAC32829.1. AK010943 mRNA. Translation: BAB27283.1. AK155910 mRNA. Translation: BAE33499.1. BC010274 mRNA. Translation: AAH10274.1.
IPI	IPI00131504.
RefSeq	NP_001156982.1. NM_001163510.1. NP_032459.3. NM_008433.4.
UniGene	Mm.9911.
3D structure databases
ProteinModelPortal	O89109.
SMR	O89109. Positions 206-373.
ModBase	Search...
Protein-protein interaction databases
STRING	O89109.
PTM databases
PhosphoSite	O89109.
Proteomic databases
PRIDE	O89109.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000067352; ENSMUSP00000070467; ENSMUSG00000054342. ENSMUST00000171904; ENSMUSP00000133065; ENSMUSG00000054342.
GeneID	16534.
KEGG	mmu:16534.
UCSC	uc009fpk.2. mouse.
Organism-specific databases
CTD	3783.
MGI	MGI:1277957. Kcnn4.
Phylogenomic databases
eggNOG	roNOG14670.
HOGENOM	HBG443547.
HOVERGEN	HBG052241.
InParanoid	O89109.
OMA	KWLAGWA.
OrthoDB	EOG42FSHQ.
Gene expression databases
ArrayExpress	O89109.
Bgee	O89109.
Genevestigator	O89109.
GermOnline	ENSMUSG00000054342. Mus musculus.
Family and domain databases
InterPro	IPR004178. CaM-bd_dom. IPR013099. Ion_trans_2. IPR015449. K_chnl_Ca-activ_SK. [Graphical view]
KO	K04945.
PANTHER	PTHR10153. CaKChannelSK. 1 hit.
Pfam	PF02888. CaMBD. 1 hit. PF07885. Ion_trans_2. 1 hit. PF03530. SK_channel. 1 hit. [Graphical view]
SMART	SM01053. CaMBD. 1 hit. [Graphical view]
SUPFAM	SSF81327. CaM_bd. 1 hit.
ProtoNet	Search...
Other
NextBio	289957.
SOURCE	Search...

Entry information

Entry name

KCNN4_MOUSE

Accession

Primary (citable) accession number: O89109
Secondary accession number(s): Q3U1J8, Q9CY39

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program