Reviewed, UniProtKB/Swiss-Prot O89106 (FHIT_MOUSE)

Names and origin

Protein names

Recommended name:
    Bis(5'-adenosyl)-triphosphatase
    EC=3.6.1.29
Alternative name(s):
    Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
    Dinucleosidetriphosphatase
    AP3A hydrolase
      Short name=AP3Aase
    Fragile histidine triad protein

Gene names

Name:

Fhit

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves A-5'-PPP-5'A to yield AMP and ADP.
Catalytic activity	P(1)-P(3)-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.
Cofactor	Divalent cations. Magnesium, but manganese and to a lesser extent calcium or cobalt can be substituted; but not zinc, cadmium or nickel By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. Ref.1
Tissue specificity	Expressed in heart, brain, lung and skeletal muscle. Particularly strong expression in liver, testis and kidney, where it is confined to the tubular epithelium. Ref.1 Ref.5
Sequence similarities	Contains 1 HIT domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA replication Inferred from direct assay. Source: MGI nucleotide metabolic process Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	bis(5'-adenosyl)-triphosphatase activity Inferred from direct assay. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 150	149	Bis(5'-adenosyl)-triphosphatase		PRO_0000109790
Regions
	Domain	2 – 109	108	HIT
	Region	82 – 99	18	Binding to substrate; phosphate linker By similarity
	Motif	94 – 98	5	Histidine triad motif By similarity
Sites
	Active site	96	1	Tele-AMP-histidine intermediate By similarity
	Binding site	27	1	Substrate By similarity
Amino acid modifications
	Modified residue	114	1	Phosphotyrosine; by SRC By similarity
	Modified residue	147	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	135	1	K → E in AAH12662. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O89106-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 27CBC57C5A4A0E2B	FASTA	150	17,235
	10 20 30 40 50 60 MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFRDL HPDEVADLFQ 70 80 90 100 110 120 VTQRVGTVVE KHFQGTSITF SMQDGPEAGQ TVKHVHVHVL PRKAGDFPRN DNIYDELQKH 130 140 150 DREEEDSPAF WRSEKEMAAE AEALRVYFQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine Fhit locus: isolation, characterization, and expression in normal and tumor cells." Pekarsky Y., Druck T., Cotticelli M.G., Ohta M., Shou J., Mendrola J., Montgomery J.C., Buchberg A.M., Siracusa L.D., Manenti G., Fong L.Y., Dragani T.A., Croce C.M., Huebner K. Cancer Res. 58:3401-3408(1998) [PubMed: 9699672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	Glover T.W., Hoge A., Miller D.E., Escara-Wilke J., Adam A.N., Dagenais S.L., Wilke C.M., Dierick H.A., Beer D.G. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: C57BL/10J. Tissue: Muscle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[4]	"Fragile site orthologs FHIT/FRA3B and Fhit/Fra14A2: evolutionarily conserved but highly recombinogenic." Matsuyama A., Shiraishi T., Trapasso F., Kuroki T., Alder H., Mori M., Huebner K., Croce C.M. Proc. Natl. Acad. Sci. U.S.A. 100:14988-14993(2003) [PubMed: 14630947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 36-150. Strain: C57BL/6J. Tissue: Kidney.
[5]	"Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans." Pekarsky Y., Campiglio M., Siprashvili Z., Druck T., Sedkov Y., Tillib S., Draganescu A., Wermuth P., Rothman J.H., Huebner K., Buchberg A.M., Mazo A., Brenner C., Croce C.M. Proc. Natl. Acad. Sci. U.S.A. 95:8744-8749(1998) [PubMed: 9671749] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF047702, AF047700, AF047701 Genomic DNA. Translation: AAC23967.1. AF047699 mRNA. Translation: AAC24566.1. AF055573 mRNA. Translation: AAC24117.1. BC012662 mRNA. Translation: AAH12662.1. AY363103 Genomic DNA. Translation: AAR17701.1.
IPI	IPI00131502.
RefSeq	NP_034340.1.
UniGene	Mm.397619 Mm.441926
3D structure databases
HSSP	HSSP built from PDB template 4FIT based on UniProtKB P49789.
SMR	O89106. Positions 1-130, 2-131.
ModBase	Search...
PTM databases
PhosphoSite	O89106.
Proteomic databases
PRIDE	O89106.
Genome annotation databases
Ensembl	ENSMUSG00000060579. Mus musculus. [Contig view]
GeneID	14198.
KEGG	mmu:14198.
Organism-specific databases
MGI	MGI:1277947. Fhit.
Phylogenomic databases
HOGENOM	O89106.
HOVERGEN	O89106.
Enzyme and pathway databases
BRENDA	3.6.1.29. 244.
Gene expression databases
Bgee	O89106.
CleanEx	MM_FHIT.
Family and domain databases
InterPro	IPR011151. His_triad_motif. IPR019808. Histidine_triad_CS. IPR001310. Histidine_triad_HIT. [Graphical view]
Gene3D	G3DSA:3.30.428.10. His_triad_motif. 1 hit.
PANTHER	PTHR23089. HIT. 1 hit.
Pfam	PF01230. HIT. 1 hit. [Graphical view]
PROSITE	PS00892. HIT_1. 1 hit. PS51084. HIT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	285422.
SOURCE	Search...

Entry information

Entry name

FHIT_MOUSE

Accession

Primary (citable) accession number: O89106
Secondary accession number(s): Q6URW5, Q91VL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)