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O89100

- GRAP2_MOUSE

UniProt

O89100 - GRAP2_MOUSE

Protein

GRB2-related adaptor protein 2

Gene

Grap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Enzyme and pathway databases

    ReactomeiREACT_188185. DAP12 signaling.
    REACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_188530. FCERI mediated MAPK activation.
    REACT_188578. Signaling by SCF-KIT.
    REACT_204081. CD28 co-stimulation.
    REACT_225768. Generation of second messenger molecules.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GRB2-related adaptor protein 2
    Alternative name(s):
    Adapter protein GRID
    GADS protein
    GRB-2-like protein
    Short name:
    GRB2L
    GRB-2-related monocytic adapter protein
    Short name:
    MONA
    Short name:
    Monocytic adapter
    GRBLG
    Growth factor receptor-binding protein
    Hematopoietic cell-associated adaptor protein GrpL
    Gene namesi
    Name:Grap2
    Synonyms:Gads, Grb2l, Grid, Mona
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1333842. Grap2.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Endosome By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. endosome Source: UniProtKB
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322GRB2-related adaptor protein 2PRO_0000088209Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei45 – 451PhosphotyrosineBy similarity
    Modified residuei106 – 1061N6-acetyllysineBy similarity
    Modified residuei254 – 2541PhosphothreonineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO89100.
    PaxDbiO89100.
    PRIDEiO89100.

    PTM databases

    PhosphoSiteiO89100.

    Expressioni

    Gene expression databases

    ArrayExpressiO89100.
    BgeeiO89100.
    CleanExiMM_GRAP2.
    GenevestigatoriO89100.

    Interactioni

    Subunit structurei

    Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23 By similarity. Interacts with phosphorylated LIME1 upon TCR activation.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LCP2Q130943EBI-642151,EBI-346946From a different organism.
    PTPN23Q9H3S72EBI-642151,EBI-724478From a different organism.

    Protein-protein interaction databases

    BioGridi201466. 10 interactions.
    DIPiDIP-41343N.
    IntActiO89100. 6 interactions.
    MINTiMINT-244269.
    STRINGi10090.ENSMUSP00000046532.

    Structurei

    Secondary structure

    1
    322
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni54 – 585
    Helixi65 – 739
    Beta strandi79 – 846
    Beta strandi86 – 883
    Beta strandi92 – 976
    Beta strandi99 – 1068
    Beta strandi115 – 1195
    Beta strandi121 – 1244
    Helixi125 – 1328
    Beta strandi137 – 1415
    Beta strandi267 – 2726
    Beta strandi278 – 2814
    Beta strandi286 – 2883
    Beta strandi289 – 2946
    Beta strandi297 – 3059
    Beta strandi308 – 3136
    Helixi314 – 3163
    Beta strandi317 – 3193

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1H3HNMR-A263-322[»]
    1OEBX-ray1.76A/B265-322[»]
    1R1PX-ray1.80A/B/C/D50-147[»]
    1R1QX-ray1.80A/B50-147[»]
    1R1SX-ray1.90A/C/E/G50-147[»]
    1UTIX-ray1.50A265-322[»]
    2D0NX-ray1.57A/C267-322[»]
    2W10X-ray1.90A/B265-322[»]
    ProteinModelPortaliO89100.
    SMRiO89100. Positions 1-150, 265-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO89100.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 5656SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini58 – 14992SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini263 – 32260SH3 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GRB2/sem-5/DRK family.Curated
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 2 SH3 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiNOG272892.
    HOGENOMiHOG000251625.
    HOVERGENiHBG005404.
    InParanoidiO89100.
    KOiK07366.
    OMAiQFPEWFH.
    OrthoDBiEOG75F4F6.
    PhylomeDBiO89100.
    TreeFamiTF354288.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00017. SH2. 1 hit.
    PF00018. SH3_1. 2 hits.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 3 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O89100-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF    50
    IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED 100
    DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ 150
    DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ 200
    QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL GSEVNATLMH 250
    RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW 300
    WTGRLHNKLG LFPANYVAPM MR 322
    Length:322
    Mass (Da):36,810
    Last modified:November 1, 1998 - v1
    Checksum:i736311D0640CD3D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055465 mRNA. Translation: AAD08803.1.
    AF053405 mRNA. Translation: AAC98669.1.
    AF129477 mRNA. Translation: AAD41783.1.
    AF236118 mRNA. Translation: AAF60318.1.
    AJ011735 mRNA. Translation: CAA09756.1.
    BC052496 mRNA. Translation: AAH52496.1.
    CCDSiCCDS27663.1.
    RefSeqiNP_001276371.1. NM_001289442.1.
    NP_034945.1. NM_010815.3.
    UniGeneiMm.12947.

    Genome annotation databases

    EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.
    GeneIDi17444.
    KEGGimmu:17444.
    UCSCiuc007wvo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055465 mRNA. Translation: AAD08803.1 .
    AF053405 mRNA. Translation: AAC98669.1 .
    AF129477 mRNA. Translation: AAD41783.1 .
    AF236118 mRNA. Translation: AAF60318.1 .
    AJ011735 mRNA. Translation: CAA09756.1 .
    BC052496 mRNA. Translation: AAH52496.1 .
    CCDSi CCDS27663.1.
    RefSeqi NP_001276371.1. NM_001289442.1.
    NP_034945.1. NM_010815.3.
    UniGenei Mm.12947.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1H3H NMR - A 263-322 [» ]
    1OEB X-ray 1.76 A/B 265-322 [» ]
    1R1P X-ray 1.80 A/B/C/D 50-147 [» ]
    1R1Q X-ray 1.80 A/B 50-147 [» ]
    1R1S X-ray 1.90 A/C/E/G 50-147 [» ]
    1UTI X-ray 1.50 A 265-322 [» ]
    2D0N X-ray 1.57 A/C 267-322 [» ]
    2W10 X-ray 1.90 A/B 265-322 [» ]
    ProteinModelPortali O89100.
    SMRi O89100. Positions 1-150, 265-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201466. 10 interactions.
    DIPi DIP-41343N.
    IntActi O89100. 6 interactions.
    MINTi MINT-244269.
    STRINGi 10090.ENSMUSP00000046532.

    PTM databases

    PhosphoSitei O89100.

    Proteomic databases

    MaxQBi O89100.
    PaxDbi O89100.
    PRIDEi O89100.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043149 ; ENSMUSP00000046532 ; ENSMUSG00000042351 .
    GeneIDi 17444.
    KEGGi mmu:17444.
    UCSCi uc007wvo.1. mouse.

    Organism-specific databases

    CTDi 9402.
    MGIi MGI:1333842. Grap2.

    Phylogenomic databases

    eggNOGi NOG272892.
    HOGENOMi HOG000251625.
    HOVERGENi HBG005404.
    InParanoidi O89100.
    KOi K07366.
    OMAi QFPEWFH.
    OrthoDBi EOG75F4F6.
    PhylomeDBi O89100.
    TreeFami TF354288.

    Enzyme and pathway databases

    Reactomei REACT_188185. DAP12 signaling.
    REACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_188530. FCERI mediated MAPK activation.
    REACT_188578. Signaling by SCF-KIT.
    REACT_204081. CD28 co-stimulation.
    REACT_225768. Generation of second messenger molecules.

    Miscellaneous databases

    EvolutionaryTracei O89100.
    NextBioi 292080.
    PROi O89100.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O89100.
    Bgeei O89100.
    CleanExi MM_GRAP2.
    Genevestigatori O89100.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00017. SH2. 1 hit.
    PF00018. SH3_1. 2 hits.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 3 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mona, a novel hematopoietic-specific adaptor interacting with the macrophage colony-stimulating factor receptor, is implicated in monocyte/macrophage development."
      Bourette R.P., Arnaud S., Myles G.M., Blanchet J.P., Rohrschneider L.R., Mouchiroud G.
      EMBO J. 17:7273-7281(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc."
      Liu S.K., McGlade C.J.
      Oncogene 17:3073-3082(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation."
      Law C.-L., Ewings M.K., Chaudhary P.M., Solow S.A., Yun T.J., Marshall A.J., Hood L., Clark E.A.
      J. Exp. Med. 189:1243-1253(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28."
      Ellis J.H., Ashman C., Burden M.N., Kilpatrick K.E., Morse M.A., Hamblin P.A.
      J. Immunol. 164:5805-5814(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Cloning of the human and mouse growth factor receptor binding protein like genes."
      Kedra D., Dumanski J.P.
      Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    7. "LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
      Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y.
      J. Exp. Med. 198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LIME1.

    Entry informationi

    Entry nameiGRAP2_MOUSE
    AccessioniPrimary (citable) accession number: O89100
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3