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Protein

GRB2-related adaptor protein 2

Gene

Grap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Enzyme and pathway databases

ReactomeiREACT_301936. DAP12 signaling.
REACT_314186. FCERI mediated MAPK activation.
REACT_327841. Signaling by SCF-KIT.
REACT_334692. Generation of second messenger molecules.
REACT_334921. CD28 co-stimulation.
REACT_348733. FCERI mediated Ca+2 mobilization.

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-related adaptor protein 2
Alternative name(s):
Adapter protein GRID
GADS protein
GRB-2-like protein
Short name:
GRB2L
GRB-2-related monocytic adapter protein
Short name:
MONA
Short name:
Monocytic adapter
GRBLG
Growth factor receptor-binding protein
Hematopoietic cell-associated adaptor protein GrpL
Gene namesi
Name:Grap2
Synonyms:Gads, Grb2l, Grid, Mona
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1333842. Grap2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322GRB2-related adaptor protein 2PRO_0000088209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451PhosphotyrosineBy similarity
Modified residuei106 – 1061N6-acetyllysineBy similarity
Modified residuei254 – 2541PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO89100.
PaxDbiO89100.
PRIDEiO89100.

PTM databases

PhosphoSiteiO89100.

Expressioni

Gene expression databases

BgeeiO89100.
CleanExiMM_GRAP2.
ExpressionAtlasiO89100. baseline and differential.
GenevestigatoriO89100.

Interactioni

Subunit structurei

Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23 (By similarity). Interacts with phosphorylated LIME1 upon TCR activation.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LCP2Q130943EBI-642151,EBI-346946From a different organism.
PTPN23Q9H3S72EBI-642151,EBI-724478From a different organism.

Protein-protein interaction databases

BioGridi201466. 10 interactions.
DIPiDIP-41343N.
IntActiO89100. 6 interactions.
MINTiMINT-244269.
STRINGi10090.ENSMUSP00000046532.

Structurei

Secondary structure

1
322
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni54 – 585Combined sources
Helixi65 – 739Combined sources
Beta strandi79 – 846Combined sources
Beta strandi86 – 883Combined sources
Beta strandi92 – 976Combined sources
Beta strandi99 – 1068Combined sources
Beta strandi115 – 1195Combined sources
Beta strandi121 – 1244Combined sources
Helixi125 – 1328Combined sources
Beta strandi137 – 1415Combined sources
Beta strandi267 – 2726Combined sources
Beta strandi278 – 2814Combined sources
Beta strandi286 – 2883Combined sources
Beta strandi289 – 2946Combined sources
Beta strandi297 – 3059Combined sources
Beta strandi308 – 3136Combined sources
Helixi314 – 3163Combined sources
Beta strandi317 – 3193Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-A263-322[»]
1OEBX-ray1.76A/B265-322[»]
1R1PX-ray1.80A/B/C/D50-147[»]
1R1QX-ray1.80A/B50-147[»]
1R1SX-ray1.90A/C/E/G50-147[»]
1UTIX-ray1.50A265-322[»]
2D0NX-ray1.57A/C267-322[»]
2W10X-ray1.90A/B265-322[»]
ProteinModelPortaliO89100.
SMRiO89100. Positions 1-150, 265-321.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO89100.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5656SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini58 – 14992SH2PROSITE-ProRule annotationAdd
BLAST
Domaini263 – 32260SH3 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiNOG272892.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiO89100.
KOiK07366.
OMAiQFPEWFH.
OrthoDBiEOG75F4F6.
PhylomeDBiO89100.
TreeFamiTF354288.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O89100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF
60 70 80 90 100
IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED
110 120 130 140 150
DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ
160 170 180 190 200
DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ
210 220 230 240 250
QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL GSEVNATLMH
260 270 280 290 300
RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW
310 320
WTGRLHNKLG LFPANYVAPM MR
Length:322
Mass (Da):36,810
Last modified:November 1, 1998 - v1
Checksum:i736311D0640CD3D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.
CCDSiCCDS27663.1.
RefSeqiNP_001276371.1. NM_001289442.1.
NP_034945.1. NM_010815.3.
UniGeneiMm.12947.

Genome annotation databases

EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.
GeneIDi17444.
KEGGimmu:17444.
UCSCiuc007wvo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.
CCDSiCCDS27663.1.
RefSeqiNP_001276371.1. NM_001289442.1.
NP_034945.1. NM_010815.3.
UniGeneiMm.12947.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-A263-322[»]
1OEBX-ray1.76A/B265-322[»]
1R1PX-ray1.80A/B/C/D50-147[»]
1R1QX-ray1.80A/B50-147[»]
1R1SX-ray1.90A/C/E/G50-147[»]
1UTIX-ray1.50A265-322[»]
2D0NX-ray1.57A/C267-322[»]
2W10X-ray1.90A/B265-322[»]
ProteinModelPortaliO89100.
SMRiO89100. Positions 1-150, 265-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201466. 10 interactions.
DIPiDIP-41343N.
IntActiO89100. 6 interactions.
MINTiMINT-244269.
STRINGi10090.ENSMUSP00000046532.

PTM databases

PhosphoSiteiO89100.

Proteomic databases

MaxQBiO89100.
PaxDbiO89100.
PRIDEiO89100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.
GeneIDi17444.
KEGGimmu:17444.
UCSCiuc007wvo.1. mouse.

Organism-specific databases

CTDi9402.
MGIiMGI:1333842. Grap2.

Phylogenomic databases

eggNOGiNOG272892.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiO89100.
KOiK07366.
OMAiQFPEWFH.
OrthoDBiEOG75F4F6.
PhylomeDBiO89100.
TreeFamiTF354288.

Enzyme and pathway databases

ReactomeiREACT_301936. DAP12 signaling.
REACT_314186. FCERI mediated MAPK activation.
REACT_327841. Signaling by SCF-KIT.
REACT_334692. Generation of second messenger molecules.
REACT_334921. CD28 co-stimulation.
REACT_348733. FCERI mediated Ca+2 mobilization.

Miscellaneous databases

EvolutionaryTraceiO89100.
NextBioi292080.
PROiO89100.
SOURCEiSearch...

Gene expression databases

BgeeiO89100.
CleanExiMM_GRAP2.
ExpressionAtlasiO89100. baseline and differential.
GenevestigatoriO89100.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mona, a novel hematopoietic-specific adaptor interacting with the macrophage colony-stimulating factor receptor, is implicated in monocyte/macrophage development."
    Bourette R.P., Arnaud S., Myles G.M., Blanchet J.P., Rohrschneider L.R., Mouchiroud G.
    EMBO J. 17:7273-7281(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc."
    Liu S.K., McGlade C.J.
    Oncogene 17:3073-3082(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation."
    Law C.-L., Ewings M.K., Chaudhary P.M., Solow S.A., Yun T.J., Marshall A.J., Hood L., Clark E.A.
    J. Exp. Med. 189:1243-1253(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28."
    Ellis J.H., Ashman C., Burden M.N., Kilpatrick K.E., Morse M.A., Hamblin P.A.
    J. Immunol. 164:5805-5814(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Cloning of the human and mouse growth factor receptor binding protein like genes."
    Kedra D., Dumanski J.P.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  7. "LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
    Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y.
    J. Exp. Med. 198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LIME1.

Entry informationi

Entry nameiGRAP2_MOUSE
AccessioniPrimary (citable) accession number: O89100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: April 1, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.