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O89100

- GRAP2_MOUSE

UniProt

O89100 - GRAP2_MOUSE

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Protein

GRB2-related adaptor protein 2

Gene

Grap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Enzyme and pathway databases

ReactomeiREACT_188185. DAP12 signaling.
REACT_188202. FCERI mediated Ca+2 mobilization.
REACT_188530. FCERI mediated MAPK activation.
REACT_188578. Signaling by SCF-KIT.
REACT_204081. CD28 co-stimulation.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-related adaptor protein 2
Alternative name(s):
Adapter protein GRID
GADS protein
GRB-2-like protein
Short name:
GRB2L
GRB-2-related monocytic adapter protein
Short name:
MONA
Short name:
Monocytic adapter
GRBLG
Growth factor receptor-binding protein
Hematopoietic cell-associated adaptor protein GrpL
Gene namesi
Name:Grap2
Synonyms:Gads, Grb2l, Grid, Mona
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1333842. Grap2.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Endosome By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endosome Source: UniProtKB
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322GRB2-related adaptor protein 2PRO_0000088209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451PhosphotyrosineBy similarity
Modified residuei106 – 1061N6-acetyllysineBy similarity
Modified residuei254 – 2541PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO89100.
PaxDbiO89100.
PRIDEiO89100.

PTM databases

PhosphoSiteiO89100.

Expressioni

Gene expression databases

BgeeiO89100.
CleanExiMM_GRAP2.
ExpressionAtlasiO89100. baseline and differential.
GenevestigatoriO89100.

Interactioni

Subunit structurei

Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23 (By similarity). Interacts with phosphorylated LIME1 upon TCR activation.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LCP2Q130943EBI-642151,EBI-346946From a different organism.
PTPN23Q9H3S72EBI-642151,EBI-724478From a different organism.

Protein-protein interaction databases

BioGridi201466. 10 interactions.
DIPiDIP-41343N.
IntActiO89100. 6 interactions.
MINTiMINT-244269.
STRINGi10090.ENSMUSP00000046532.

Structurei

Secondary structure

1
322
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni54 – 585Combined sources
Helixi65 – 739Combined sources
Beta strandi79 – 846Combined sources
Beta strandi86 – 883Combined sources
Beta strandi92 – 976Combined sources
Beta strandi99 – 1068Combined sources
Beta strandi115 – 1195Combined sources
Beta strandi121 – 1244Combined sources
Helixi125 – 1328Combined sources
Beta strandi137 – 1415Combined sources
Beta strandi267 – 2726Combined sources
Beta strandi278 – 2814Combined sources
Beta strandi286 – 2883Combined sources
Beta strandi289 – 2946Combined sources
Beta strandi297 – 3059Combined sources
Beta strandi308 – 3136Combined sources
Helixi314 – 3163Combined sources
Beta strandi317 – 3193Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-A263-322[»]
1OEBX-ray1.76A/B265-322[»]
1R1PX-ray1.80A/B/C/D50-147[»]
1R1QX-ray1.80A/B50-147[»]
1R1SX-ray1.90A/C/E/G50-147[»]
1UTIX-ray1.50A265-322[»]
2D0NX-ray1.57A/C267-322[»]
2W10X-ray1.90A/B265-322[»]
ProteinModelPortaliO89100.
SMRiO89100. Positions 1-150, 265-321.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO89100.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5656SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini58 – 14992SH2PROSITE-ProRule annotationAdd
BLAST
Domaini263 – 32260SH3 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiNOG272892.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiO89100.
KOiK07366.
OMAiQFPEWFH.
OrthoDBiEOG75F4F6.
PhylomeDBiO89100.
TreeFamiTF354288.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O89100-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF
60 70 80 90 100
IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED
110 120 130 140 150
DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ
160 170 180 190 200
DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ
210 220 230 240 250
QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL GSEVNATLMH
260 270 280 290 300
RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW
310 320
WTGRLHNKLG LFPANYVAPM MR
Length:322
Mass (Da):36,810
Last modified:November 1, 1998 - v1
Checksum:i736311D0640CD3D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.
CCDSiCCDS27663.1.
RefSeqiNP_001276371.1. NM_001289442.1.
NP_034945.1. NM_010815.3.
UniGeneiMm.12947.

Genome annotation databases

EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.
GeneIDi17444.
KEGGimmu:17444.
UCSCiuc007wvo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA. Translation: AAD08803.1 .
AF053405 mRNA. Translation: AAC98669.1 .
AF129477 mRNA. Translation: AAD41783.1 .
AF236118 mRNA. Translation: AAF60318.1 .
AJ011735 mRNA. Translation: CAA09756.1 .
BC052496 mRNA. Translation: AAH52496.1 .
CCDSi CCDS27663.1.
RefSeqi NP_001276371.1. NM_001289442.1.
NP_034945.1. NM_010815.3.
UniGenei Mm.12947.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1H3H NMR - A 263-322 [» ]
1OEB X-ray 1.76 A/B 265-322 [» ]
1R1P X-ray 1.80 A/B/C/D 50-147 [» ]
1R1Q X-ray 1.80 A/B 50-147 [» ]
1R1S X-ray 1.90 A/C/E/G 50-147 [» ]
1UTI X-ray 1.50 A 265-322 [» ]
2D0N X-ray 1.57 A/C 267-322 [» ]
2W10 X-ray 1.90 A/B 265-322 [» ]
ProteinModelPortali O89100.
SMRi O89100. Positions 1-150, 265-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201466. 10 interactions.
DIPi DIP-41343N.
IntActi O89100. 6 interactions.
MINTi MINT-244269.
STRINGi 10090.ENSMUSP00000046532.

PTM databases

PhosphoSitei O89100.

Proteomic databases

MaxQBi O89100.
PaxDbi O89100.
PRIDEi O89100.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043149 ; ENSMUSP00000046532 ; ENSMUSG00000042351 .
GeneIDi 17444.
KEGGi mmu:17444.
UCSCi uc007wvo.1. mouse.

Organism-specific databases

CTDi 9402.
MGIi MGI:1333842. Grap2.

Phylogenomic databases

eggNOGi NOG272892.
HOGENOMi HOG000251625.
HOVERGENi HBG005404.
InParanoidi O89100.
KOi K07366.
OMAi QFPEWFH.
OrthoDBi EOG75F4F6.
PhylomeDBi O89100.
TreeFami TF354288.

Enzyme and pathway databases

Reactomei REACT_188185. DAP12 signaling.
REACT_188202. FCERI mediated Ca+2 mobilization.
REACT_188530. FCERI mediated MAPK activation.
REACT_188578. Signaling by SCF-KIT.
REACT_204081. CD28 co-stimulation.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

EvolutionaryTracei O89100.
NextBioi 292080.
PROi O89100.
SOURCEi Search...

Gene expression databases

Bgeei O89100.
CleanExi MM_GRAP2.
ExpressionAtlasi O89100. baseline and differential.
Genevestigatori O89100.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEi PS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mona, a novel hematopoietic-specific adaptor interacting with the macrophage colony-stimulating factor receptor, is implicated in monocyte/macrophage development."
    Bourette R.P., Arnaud S., Myles G.M., Blanchet J.P., Rohrschneider L.R., Mouchiroud G.
    EMBO J. 17:7273-7281(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc."
    Liu S.K., McGlade C.J.
    Oncogene 17:3073-3082(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation."
    Law C.-L., Ewings M.K., Chaudhary P.M., Solow S.A., Yun T.J., Marshall A.J., Hood L., Clark E.A.
    J. Exp. Med. 189:1243-1253(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28."
    Ellis J.H., Ashman C., Burden M.N., Kilpatrick K.E., Morse M.A., Hamblin P.A.
    J. Immunol. 164:5805-5814(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Cloning of the human and mouse growth factor receptor binding protein like genes."
    Kedra D., Dumanski J.P.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  7. "LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
    Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y.
    J. Exp. Med. 198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LIME1.

Entry informationi

Entry nameiGRAP2_MOUSE
AccessioniPrimary (citable) accession number: O89100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 26, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3