GRB2-related adaptor protein 2 - Mus musculus (Mouse)

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O89100 (GRAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GRB2-related adaptor protein 2

Alternative name(s):
Adapter protein GRID
GADS protein
GRB-2-like protein
Short name=GRB2L
GRB-2-related monocytic adapter protein
Short name=MONA
Short name=Monocytic adapter
GRBLG
Growth factor receptor-binding protein
Hematopoietic cell-associated adaptor protein GrpL

Gene names

Name:	Grap2
Synonyms:	Gads, Grb2l, Grid, Mona

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
Subunit structure	Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23 By similarity. Interacts with phosphorylated LIME1 upon TCR activation. Ref.7
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Endosome By similarity.
Sequence similarities	Belongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.

Ontologies

Keywords
Cellular component	Cytoplasm Endosome Nucleus
Domain	Repeat SH2 domain SH3 domain
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endosome Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 322

322

GRB2-related adaptor protein 2

PRO_0000088209

Regions

Domain

1 – 56

SH3 1

Domain

58 – 149

SH2

Domain

263 – 322

SH3 2

Amino acid modifications

Modified residue

Phosphotyrosine By similarity

Modified residue

106

N6-acetyllysine By similarity

Modified residue

254

Phosphothreonine By similarity

Secondary structure

322

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O89100 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 736311D0640CD3D0
Show »

FASTA

322

36,810

        10         20         30         40         50         60 
MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF IDIEFPEWFH 

        70         80         90        100        110        120 
EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD TKGNYFLWTE 

       130        140        150        160        170        180 
KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS 

       190        200        210        220        230        240 
VNRKLSDHLP LGPQQFHPHQ QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL 

       250        260        270        280        290        300 
GSEVNATLMH RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW 

       310        320 
WTGRLHNKLG LFPANYVAPM MR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mona, a novel hematopoietic-specific adaptor interacting with the macrophage colony-stimulating factor receptor, is implicated in monocyte/macrophage development." Bourette R.P., Arnaud S., Myles G.M., Blanchet J.P., Rohrschneider L.R., Mouchiroud G. EMBO J. 17:7273-7281(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc." Liu S.K., McGlade C.J. Oncogene 17:3073-3082(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation." Law C.-L., Ewings M.K., Chaudhary P.M., Solow S.A., Yun T.J., Marshall A.J., Hood L., Clark E.A. J. Exp. Med. 189:1243-1253(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28." Ellis J.H., Ashman C., Burden M.N., Kilpatrick K.E., Morse M.A., Hamblin P.A. J. Immunol. 164:5805-5814(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cloning of the human and mouse growth factor receptor binding protein like genes." Kedra D., Dumanski J.P. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[7]	"LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation." Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y. J. Exp. Med. 198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LIME1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.

IPI

IPI00131492.

RefSeq

NP_034945.1. NM_010815.2.

UniGene

Mm.12947.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H3H	NMR	-	A	263-322	[»]
1OEB	X-ray	1.76	A/B	265-322	[»]
1R1P	X-ray	1.80	A/B/C/D	50-147	[»]
1R1Q	X-ray	1.80	A/B	50-147	[»]
1R1S	X-ray	1.90	A/C/E/G	50-147	[»]
1UTI	X-ray	1.50	A	265-322	[»]
2D0N	X-ray	1.57	A/C	267-322	[»]
2W10	X-ray	1.90	A/B	265-322	[»]

ProteinModelPortal

O89100.

SMR

O89100. Positions 1-150, 265-321.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-41343N.

IntAct

O89100. 1 interaction.

MINT

MINT-244269.

STRING

10090.ENSMUSP00000046532.

PTM databases

PhosphoSite

O89100.

Proteomic databases

PaxDb

O89100.

PRIDE

O89100.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.

GeneID

17444.

KEGG

mmu:17444.

Organism-specific databases

CTD

9402.

MGI

MGI:1333842. Grap2.

Phylogenomic databases

eggNOG

NOG272892.

HOGENOM

HOG000251625.

HOVERGEN

HBG005404.

InParanoid

O89100.

K07366.

OMA

HTGDILK.

OrthoDB

EOG4BCDND.

Gene expression databases

ArrayExpress

O89100.

Bgee

O89100.

CleanEx

MM_GRAP2.

Genevestigator

O89100.

GermOnline

ENSMUSG00000042351. Mus musculus.

Family and domain databases

Gene3D

3.30.505.10. 1 hit.

InterPro

IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.

SMART

SM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]

SUPFAM

SSF50044. SH3. 2 hits.

PROSITE

PS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O89100.

NextBio

292080.

SOURCE

Search...

Entry information

Entry name

GRAP2_MOUSE

Accession

Primary (citable) accession number: O89100

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents