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Protein

GRB2-related adaptor protein 2

Gene

Grap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Enzyme and pathway databases

ReactomeiR-MMU-1433557. Signaling by SCF-KIT.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-2424491. DAP12 signaling.
R-MMU-2871796. FCERI mediated MAPK activation.
R-MMU-2871809. FCERI mediated Ca+2 mobilization.
R-MMU-389356. CD28 co-stimulation.

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-related adaptor protein 2
Alternative name(s):
Adapter protein GRID
GADS protein
GRB-2-like protein
Short name:
GRB2L
GRB-2-related monocytic adapter protein
Short name:
MONA
Short name:
Monocytic adapter
GRBLG
Growth factor receptor-binding protein
Hematopoietic cell-associated adaptor protein GrpL
Gene namesi
Name:Grap2
Synonyms:Gads, Grb2l, Grid, Mona
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1333842. Grap2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882091 – 322GRB2-related adaptor protein 2Add BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphotyrosineBy similarity1
Modified residuei106N6-acetyllysineBy similarity1
Modified residuei186PhosphoserineCombined sources1
Modified residuei230PhosphoserineCombined sources1
Modified residuei254PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO89100.
PaxDbiO89100.
PRIDEiO89100.

PTM databases

iPTMnetiO89100.
PhosphoSitePlusiO89100.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042351.
CleanExiMM_GRAP2.
ExpressionAtlasiO89100. baseline and differential.
GenevisibleiO89100. MM.

Interactioni

Subunit structurei

Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23 (By similarity). Interacts with phosphorylated LIME1 upon TCR activation.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LCP2Q130943EBI-642151,EBI-346946From a different organism.
PTPN23Q9H3S72EBI-642151,EBI-724478From a different organism.

Protein-protein interaction databases

BioGridi201466. 9 interactors.
DIPiDIP-41343N.
IntActiO89100. 6 interactors.
MINTiMINT-244269.
STRINGi10090.ENSMUSP00000046532.

Structurei

Secondary structure

1322
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni54 – 58Combined sources5
Helixi65 – 73Combined sources9
Beta strandi79 – 84Combined sources6
Beta strandi86 – 88Combined sources3
Beta strandi92 – 97Combined sources6
Beta strandi99 – 106Combined sources8
Beta strandi115 – 119Combined sources5
Beta strandi121 – 124Combined sources4
Helixi125 – 132Combined sources8
Beta strandi137 – 141Combined sources5
Beta strandi267 – 272Combined sources6
Beta strandi278 – 281Combined sources4
Beta strandi286 – 288Combined sources3
Beta strandi289 – 294Combined sources6
Beta strandi297 – 305Combined sources9
Beta strandi308 – 313Combined sources6
Helixi314 – 316Combined sources3
Beta strandi317 – 319Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-A263-322[»]
1OEBX-ray1.76A/B265-322[»]
1R1PX-ray1.80A/B/C/D50-147[»]
1R1QX-ray1.80A/B50-147[»]
1R1SX-ray1.90A/C/E/G50-147[»]
1UTIX-ray1.50A265-322[»]
2D0NX-ray1.57A/C267-322[»]
2W10X-ray1.90A/B265-322[»]
ProteinModelPortaliO89100.
SMRiO89100.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO89100.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 56SH3 1PROSITE-ProRule annotationAdd BLAST56
Domaini58 – 149SH2PROSITE-ProRule annotationAdd BLAST92
Domaini263 – 322SH3 2PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3601. Eukaryota.
ENOG410XR1G. LUCA.
GeneTreeiENSGT00820000126999.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiO89100.
KOiK07366.
OMAiLSSQEEW.
OrthoDBiEOG091G0HWS.
PhylomeDBiO89100.
TreeFamiTF354288.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O89100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF
60 70 80 90 100
IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED
110 120 130 140 150
DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ
160 170 180 190 200
DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ
210 220 230 240 250
QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL GSEVNATLMH
260 270 280 290 300
RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW
310 320
WTGRLHNKLG LFPANYVAPM MR
Length:322
Mass (Da):36,810
Last modified:November 1, 1998 - v1
Checksum:i736311D0640CD3D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.
CCDSiCCDS27663.1.
RefSeqiNP_001276371.1. NM_001289442.1.
NP_034945.1. NM_010815.3.
UniGeneiMm.12947.

Genome annotation databases

EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.
GeneIDi17444.
KEGGimmu:17444.
UCSCiuc007wvo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.
CCDSiCCDS27663.1.
RefSeqiNP_001276371.1. NM_001289442.1.
NP_034945.1. NM_010815.3.
UniGeneiMm.12947.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-A263-322[»]
1OEBX-ray1.76A/B265-322[»]
1R1PX-ray1.80A/B/C/D50-147[»]
1R1QX-ray1.80A/B50-147[»]
1R1SX-ray1.90A/C/E/G50-147[»]
1UTIX-ray1.50A265-322[»]
2D0NX-ray1.57A/C267-322[»]
2W10X-ray1.90A/B265-322[»]
ProteinModelPortaliO89100.
SMRiO89100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201466. 9 interactors.
DIPiDIP-41343N.
IntActiO89100. 6 interactors.
MINTiMINT-244269.
STRINGi10090.ENSMUSP00000046532.

PTM databases

iPTMnetiO89100.
PhosphoSitePlusiO89100.

Proteomic databases

EPDiO89100.
PaxDbiO89100.
PRIDEiO89100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.
GeneIDi17444.
KEGGimmu:17444.
UCSCiuc007wvo.2. mouse.

Organism-specific databases

CTDi9402.
MGIiMGI:1333842. Grap2.

Phylogenomic databases

eggNOGiKOG3601. Eukaryota.
ENOG410XR1G. LUCA.
GeneTreeiENSGT00820000126999.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiO89100.
KOiK07366.
OMAiLSSQEEW.
OrthoDBiEOG091G0HWS.
PhylomeDBiO89100.
TreeFamiTF354288.

Enzyme and pathway databases

ReactomeiR-MMU-1433557. Signaling by SCF-KIT.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-2424491. DAP12 signaling.
R-MMU-2871796. FCERI mediated MAPK activation.
R-MMU-2871809. FCERI mediated Ca+2 mobilization.
R-MMU-389356. CD28 co-stimulation.

Miscellaneous databases

EvolutionaryTraceiO89100.
PROiO89100.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042351.
CleanExiMM_GRAP2.
ExpressionAtlasiO89100. baseline and differential.
GenevisibleiO89100. MM.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRAP2_MOUSE
AccessioniPrimary (citable) accession number: O89100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.