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O89100 (GRAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GRB2-related adaptor protein 2
Alternative name(s):
Adapter protein GRID
GADS protein
GRB-2-like protein
Short name=GRB2L
GRB-2-related monocytic adapter protein
Short name=MONA
Short name=Monocytic adapter
GRBLG
Growth factor receptor-binding protein
Hematopoietic cell-associated adaptor protein GrpL
Gene names
Name:Grap2
Synonyms:Gads, Grb2l, Grid, Mona
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Subunit structure

Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23 By similarity. Interacts with phosphorylated LIME1 upon TCR activation. Ref.7

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Endosome By similarity.

Sequence similarities

Belongs to the GRB2/sem-5/DRK family.

Contains 1 SH2 domain.

Contains 2 SH3 domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LCP2Q130943EBI-642151,EBI-346946From a different organism.
PTPN23Q9H3S72EBI-642151,EBI-724478From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322GRB2-related adaptor protein 2
PRO_0000088209

Regions

Domain1 – 5656SH3 1
Domain58 – 14992SH2
Domain263 – 32260SH3 2

Amino acid modifications

Modified residue451Phosphotyrosine By similarity
Modified residue1061N6-acetyllysine By similarity
Modified residue2541Phosphothreonine By similarity

Secondary structure

................................. 322
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O89100 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 736311D0640CD3D0

FASTA32236,810
        10         20         30         40         50         60 
MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF IDIEFPEWFH 

        70         80         90        100        110        120 
EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD TKGNYFLWTE 

       130        140        150        160        170        180 
KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS 

       190        200        210        220        230        240 
VNRKLSDHLP LGPQQFHPHQ QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL 

       250        260        270        280        290        300 
GSEVNATLMH RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW 

       310        320 
WTGRLHNKLG LFPANYVAPM MR 

« Hide

References

« Hide 'large scale' references
[1]"Mona, a novel hematopoietic-specific adaptor interacting with the macrophage colony-stimulating factor receptor, is implicated in monocyte/macrophage development."
Bourette R.P., Arnaud S., Myles G.M., Blanchet J.P., Rohrschneider L.R., Mouchiroud G.
EMBO J. 17:7273-7281(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc."
Liu S.K., McGlade C.J.
Oncogene 17:3073-3082(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation."
Law C.-L., Ewings M.K., Chaudhary P.M., Solow S.A., Yun T.J., Marshall A.J., Hood L., Clark E.A.
J. Exp. Med. 189:1243-1253(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28."
Ellis J.H., Ashman C., Burden M.N., Kilpatrick K.E., Morse M.A., Hamblin P.A.
J. Immunol. 164:5805-5814(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Cloning of the human and mouse growth factor receptor binding protein like genes."
Kedra D., Dumanski J.P.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Thymus.
[7]"LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y.
J. Exp. Med. 198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LIME1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.
CCDSCCDS27663.1.
RefSeqNP_001276371.1. NM_001289442.1.
NP_034945.1. NM_010815.3.
UniGeneMm.12947.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-A263-322[»]
1OEBX-ray1.76A/B265-322[»]
1R1PX-ray1.80A/B/C/D50-147[»]
1R1QX-ray1.80A/B50-147[»]
1R1SX-ray1.90A/C/E/G50-147[»]
1UTIX-ray1.50A265-322[»]
2D0NX-ray1.57A/C267-322[»]
2W10X-ray1.90A/B265-322[»]
ProteinModelPortalO89100.
SMRO89100. Positions 1-150, 265-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201466. 10 interactions.
DIPDIP-41343N.
IntActO89100. 6 interactions.
MINTMINT-244269.
STRING10090.ENSMUSP00000046532.

PTM databases

PhosphoSiteO89100.

Proteomic databases

MaxQBO89100.
PaxDbO89100.
PRIDEO89100.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351.
GeneID17444.
KEGGmmu:17444.
UCSCuc007wvo.1. mouse.

Organism-specific databases

CTD9402.
MGIMGI:1333842. Grap2.

Phylogenomic databases

eggNOGNOG272892.
HOGENOMHOG000251625.
HOVERGENHBG005404.
InParanoidO89100.
KOK07366.
OMAQFPEWFH.
OrthoDBEOG75F4F6.
PhylomeDBO89100.
TreeFamTF354288.

Gene expression databases

ArrayExpressO89100.
BgeeO89100.
CleanExMM_GRAP2.
GenevestigatorO89100.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO89100.
NextBio292080.
PROO89100.
SOURCESearch...

Entry information

Entry nameGRAP2_MOUSE
AccessionPrimary (citable) accession number: O89100
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot