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Protein

Caspase-14

Gene

Casp14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and is required for cornification (PubMed:18156206). Regulates maturation of the epidermis by proteolytically processing filaggrin (PubMed:21654840). In vitro is equally active on the synthetic caspase substrates WEHD-ACF and IETD-AFC. Involved in processing of prosaposin in the epidermis (PubMed:24872419). May be involved in retinal pigment epithelium cell barrier function (By similarity).By similarity5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931By similarity
Active sitei136 – 1361By similarity

GO - Molecular functioni

  • cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
  • peptidase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Differentiation

Protein family/group databases

MEROPSiC14.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-14 (EC:3.4.22.-)
Short name:
CASP-14
Alternative name(s):
Mini-ICE
Short name:
MICE
Cleaved into the following 4 chains:
Gene namesi
Name:Casp14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1335092. Casp14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show a shiny and lichenified skin with an epidermis containing more alveolar keratohyalin F-granules and an altered profilaggrin processing. The skin is highly sensitive to the formation of cyclobutane pyrimidine dimers after UVB irradiation, leading to increased levels of UVB-induced apoptosis (PubMed:17515931). Mice accumulate incomplete filaggrin breakdown products within the epidermal stratum corneum (SC), leading to reduced levels of natural moisturizing factors (NMFs) and lower SC hydration (PubMed:21654840).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi136 – 1361C → A: Decrease in death-inducing activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 155Caspase-14 subunit p17, mature formBy similarityPRO_0000432798
Chaini1 – 193193Caspase-14 subunit p20, intermediate formBy similarityPRO_0000432797Add
BLAST
Propeptidei1 – ?CuratedPRO_0000004655
Propeptidei156 – 16712By similarityPRO_0000432799Add
BLAST
Chaini168 – 25790Caspase-14 subunit p10, mature formBy similarityPRO_0000004657Add
BLAST
Chaini194 – 25764Caspase-14 subunit p8, intermediate formBy similarityPRO_0000432800Add
BLAST

Post-translational modificationi

Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts the precursor to yield the p17/p10 mature form (By similarity). Initially it was reported that cleavage by granzyme B, caspase-8 and -10 generates the two active subunits, however the physiological relevance has not been established (PubMed:9823333).By similarity1 Publication

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiO89094.
PRIDEiO89094.

Expressioni

Tissue specificityi

Embryo, adult liver and less in adult brain and kidney. Expressed in differentiating keratinocytes of embryonic skin (at protein level). Expressed in keratinocytes of adult skin suprabasal layers (at protein level).2 Publications

Developmental stagei

Expressed at embryonic day 18 specifically in cornified epithelium in the suprabasal layers.1 Publication

Gene expression databases

BgeeiENSMUSG00000005355.
CleanExiMM_CASP14.
ExpressionAtlasiO89094. baseline and differential.
GenevisibleiO89094. MM.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit, both processed from the precursor; the mature active form is a p17/p10 dimer and the intermediate form a p20/p8 dimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005488.

Structurei

3D structure databases

ProteinModelPortaliO89094.
SMRiO89094. Positions 32-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00760000118912.
HOGENOMiHOG000231878.
HOVERGENiHBG050804.
InParanoidiO89094.
KOiK04401.
OMAiLMANTEV.
OrthoDBiEOG091G0KY9.
PhylomeDBiO89094.
TreeFamiTF102023.

Family and domain databases

Gene3Di3.40.50.1460. 1 hit.
InterProiIPR033174. Caspase-14.
IPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
[Graphical view]
PANTHERiPTHR10454:SF131. PTHR10454:SF131. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O89094-1) [UniParc]FASTAAdd to basket
Also known as: C14L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESEMSDPQP LQEERYDMSG ARLALTLCVT KAREGSEVDM EALERMFRYL
60 70 80 90 100
KFESTMKRDP TAQQFLEELD EFQQTIDNWE EPVSCAFVVL MAHGEEGLLK
110 120 130 140 150
GEDEKMVRLE DLFEVLNNKN CKALRGKPKV YIIQACRGEH RDPGEELRGN
160 170 180 190 200
EELGGDEELG GDEVAVLKNN PQSIPTYTDT LHIYSTVEGY LSYRHDEKGS
210 220 230 240 250
GFIQTLTDVF IHKKGSILEL TEEITRLMAN TEVMQEGKPR KVNPEVQSTL

RKKLYLQ
Length:257
Mass (Da):29,458
Last modified:November 1, 1998 - v1
Checksum:iA228D88DFBA0EB84
GO
Isoform 2 (identifier: O89094-2) [UniParc]FASTAAdd to basket
Also known as: C14S

The sequence of this isoform differs from the canonical sequence as follows:
     139-159: EHRDPGEELRGNEELGGDEEL → DHPTYGKHGGDAGRKTKESEP
     160-257: Missing.

Show »
Length:159
Mass (Da):18,224
Checksum:iF678D51FD9A518B4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei139 – 15921EHRDP…GDEEL → DHPTYGKHGGDAGRKTKESE P in isoform 2. CuratedVSP_028925Add
BLAST
Alternative sequencei160 – 25798Missing in isoform 2. CuratedVSP_028926Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092997 mRNA. Translation: AAC63364.1.
AJ007750 mRNA. Translation: CAA07678.1.
CCDSiCCDS23969.1. [O89094-1]
RefSeqiNP_033939.1. NM_009809.5. [O89094-1]
XP_006513222.1. XM_006513159.1. [O89094-1]
XP_006513223.1. XM_006513160.2. [O89094-2]
UniGeneiMm.20940.

Genome annotation databases

EnsembliENSMUST00000005488; ENSMUSP00000005488; ENSMUSG00000005355. [O89094-1]
GeneIDi12365.
KEGGimmu:12365.
UCSCiuc007fyd.2. mouse. [O89094-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092997 mRNA. Translation: AAC63364.1.
AJ007750 mRNA. Translation: CAA07678.1.
CCDSiCCDS23969.1. [O89094-1]
RefSeqiNP_033939.1. NM_009809.5. [O89094-1]
XP_006513222.1. XM_006513159.1. [O89094-1]
XP_006513223.1. XM_006513160.2. [O89094-2]
UniGeneiMm.20940.

3D structure databases

ProteinModelPortaliO89094.
SMRiO89094. Positions 32-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005488.

Protein family/group databases

MEROPSiC14.018.

Proteomic databases

PaxDbiO89094.
PRIDEiO89094.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005488; ENSMUSP00000005488; ENSMUSG00000005355. [O89094-1]
GeneIDi12365.
KEGGimmu:12365.
UCSCiuc007fyd.2. mouse. [O89094-1]

Organism-specific databases

CTDi23581.
MGIiMGI:1335092. Casp14.

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00760000118912.
HOGENOMiHOG000231878.
HOVERGENiHBG050804.
InParanoidiO89094.
KOiK04401.
OMAiLMANTEV.
OrthoDBiEOG091G0KY9.
PhylomeDBiO89094.
TreeFamiTF102023.

Miscellaneous databases

PROiO89094.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005355.
CleanExiMM_CASP14.
ExpressionAtlasiO89094. baseline and differential.
GenevisibleiO89094. MM.

Family and domain databases

Gene3Di3.40.50.1460. 1 hit.
InterProiIPR033174. Caspase-14.
IPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
[Graphical view]
PANTHERiPTHR10454:SF131. PTHR10454:SF131. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF52129. SSF52129. 1 hit.
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCASPE_MOUSE
AccessioniPrimary (citable) accession number: O89094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.