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Protein

Caspase-14

Gene

Casp14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and is required for cornification (PubMed:18156206). Regulates maturation of the epidermis by proteolytically processing filaggrin (PubMed:21654840). In vitro is equally active on the synthetic caspase substrates WEHD-ACF and IETD-AFC. Involved in processing of prosaposin in the epidermis (PubMed:24872419). May be involved in retinal pigment epithelium cell barrier function (By similarity).By similarity5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93By similarity1
Active sitei136By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processDifferentiation

Enzyme and pathway databases

ReactomeiR-MMU-6809371 Formation of the cornified envelope

Protein family/group databases

MEROPSiC14.018

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-14 (EC:3.4.22.-)
Short name:
CASP-14
Alternative name(s):
Mini-ICE
Short name:
MICE
Cleaved into the following 4 chains:
Gene namesi
Name:Casp14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1335092 Casp14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show a shiny and lichenified skin with an epidermis containing more alveolar keratohyalin F-granules and an altered profilaggrin processing. The skin is highly sensitive to the formation of cyclobutane pyrimidine dimers after UVB irradiation, leading to increased levels of UVB-induced apoptosis (PubMed:17515931). Mice accumulate incomplete filaggrin breakdown products within the epidermal stratum corneum (SC), leading to reduced levels of natural moisturizing factors (NMFs) and lower SC hydration (PubMed:21654840).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136C → A: Decrease in death-inducing activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000432798? – 155Caspase-14 subunit p17, mature formBy similarity
ChainiPRO_00004327971 – 193Caspase-14 subunit p20, intermediate formBy similarityAdd BLAST193
PropeptideiPRO_00000046551 – ?Curated
PropeptideiPRO_0000432799156 – 167By similarityAdd BLAST12
ChainiPRO_0000004657168 – 257Caspase-14 subunit p10, mature formBy similarityAdd BLAST90
ChainiPRO_0000432800194 – 257Caspase-14 subunit p8, intermediate formBy similarityAdd BLAST64

Post-translational modificationi

Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts the precursor to yield the p17/p10 mature form (By similarity). Initially it was reported that cleavage by granzyme B, caspase-8 and -10 generates the two active subunits, however the physiological relevance has not been established (PubMed:9823333).By similarity1 Publication

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiO89094
PRIDEiO89094

PTM databases

CarbonylDBiO89094
PhosphoSitePlusiO89094

Expressioni

Tissue specificityi

Embryo, adult liver and less in adult brain and kidney. Expressed in differentiating keratinocytes of embryonic skin (at protein level). Expressed in keratinocytes of adult skin suprabasal layers (at protein level).2 Publications

Developmental stagei

Expressed at embryonic day 18 specifically in cornified epithelium in the suprabasal layers.1 Publication

Gene expression databases

BgeeiENSMUSG00000005355
CleanExiMM_CASP14
ExpressionAtlasiO89094 baseline and differential
GenevisibleiO89094 MM

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit, both processed from the precursor; the mature active form is a p17/p10 dimer and the intermediate form a p20/p8 dimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005488

Structurei

3D structure databases

ProteinModelPortaliO89094
SMRiO89094
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiKOG3573 Eukaryota
ENOG410ZQIE LUCA
GeneTreeiENSGT00760000118912
HOGENOMiHOG000231878
HOVERGENiHBG050804
InParanoidiO89094
KOiK04401
OMAiSTMKRDP
OrthoDBiEOG091G0KY9
PhylomeDBiO89094
TreeFamiTF102023

Family and domain databases

CDDicd00032 CASc, 1 hit
InterProiView protein in InterPro
IPR033174 Caspase-14
IPR029030 Caspase-like_dom_sf
IPR033139 Caspase_cys_AS
IPR002138 Pept_C14_p10
IPR001309 Pept_C14_p20
IPR015917 Pept_C14A
PANTHERiPTHR10454:SF131 PTHR10454:SF131, 1 hit
PRINTSiPR00376 IL1BCENZYME
SMARTiView protein in SMART
SM00115 CASc, 1 hit
SUPFAMiSSF52129 SSF52129, 1 hit
PROSITEiView protein in PROSITE
PS01122 CASPASE_CYS, 1 hit
PS50207 CASPASE_P10, 1 hit
PS50208 CASPASE_P20, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O89094-1) [UniParc]FASTAAdd to basket
Also known as: C14L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESEMSDPQP LQEERYDMSG ARLALTLCVT KAREGSEVDM EALERMFRYL
60 70 80 90 100
KFESTMKRDP TAQQFLEELD EFQQTIDNWE EPVSCAFVVL MAHGEEGLLK
110 120 130 140 150
GEDEKMVRLE DLFEVLNNKN CKALRGKPKV YIIQACRGEH RDPGEELRGN
160 170 180 190 200
EELGGDEELG GDEVAVLKNN PQSIPTYTDT LHIYSTVEGY LSYRHDEKGS
210 220 230 240 250
GFIQTLTDVF IHKKGSILEL TEEITRLMAN TEVMQEGKPR KVNPEVQSTL

RKKLYLQ
Length:257
Mass (Da):29,458
Last modified:November 1, 1998 - v1
Checksum:iA228D88DFBA0EB84
GO
Isoform 2 (identifier: O89094-2) [UniParc]FASTAAdd to basket
Also known as: C14S

The sequence of this isoform differs from the canonical sequence as follows:
     139-159: EHRDPGEELRGNEELGGDEEL → DHPTYGKHGGDAGRKTKESEP
     160-257: Missing.

Show »
Length:159
Mass (Da):18,224
Checksum:iF678D51FD9A518B4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028925139 – 159EHRDP…GDEEL → DHPTYGKHGGDAGRKTKESE P in isoform 2. CuratedAdd BLAST21
Alternative sequenceiVSP_028926160 – 257Missing in isoform 2. CuratedAdd BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092997 mRNA Translation: AAC63364.1
AJ007750 mRNA Translation: CAA07678.1
CCDSiCCDS23969.1 [O89094-1]
RefSeqiNP_033939.1, NM_009809.5 [O89094-1]
XP_006513223.1, XM_006513160.3 [O89094-2]
UniGeneiMm.20940

Genome annotation databases

EnsembliENSMUST00000005488; ENSMUSP00000005488; ENSMUSG00000005355 [O89094-1]
ENSMUST00000219237; ENSMUSP00000151657; ENSMUSG00000005355 [O89094-1]
GeneIDi12365
KEGGimmu:12365
UCSCiuc007fyd.2 mouse [O89094-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCASPE_MOUSE
AccessioniPrimary (citable) accession number: O89094
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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