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Protein

C-C motif chemokine 20

Gene

Ccl20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.By similarity

GO - Molecular functioni

  1. cytokine activity Source: MGI

GO - Biological processi

  1. cell chemotaxis Source: GOC
  2. cellular response to lipopolysaccharide Source: Ensembl
  3. cellular response to lipoteichoic acid Source: Ensembl
  4. immune response Source: InterPro
  5. inflammatory response Source: UniProtKB-KW
  6. positive regulation of interleukin-1 alpha biosynthetic process Source: Ensembl
  7. positive regulation of nitric-oxide synthase biosynthetic process Source: Ensembl
  8. positive regulation of T cell migration Source: MGI
  9. wound healing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Enzyme and pathway databases

ReactomeiREACT_230248. Chemokine receptors bind chemokines.
REACT_250376. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 20
Alternative name(s):
Beta-chemokine exodus-1
CC chemokine LARC
CC chemokine ST38
Liver and activation-regulated chemokine
Macrophage inflammatory protein 3 alpha
Short name:
MIP-3-alpha
Small-inducible cytokine A20
Gene namesi
Name:Ccl20
Synonyms:Larc, Scya20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1329031. Ccl20.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 9770C-C motif chemokine 20PRO_0000005218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 591 Publication
Disulfide bondi34 ↔ 751 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO89093.

Expressioni

Tissue specificityi

Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues.

Inductioni

By lipopolysaccharide (LPS), TNF-alpha and interleukin-1. IFN-gamma alone showed no effect, but potentiated the effect of TNF.

Gene expression databases

BgeeiO89093.
CleanExiMM_CCL20.
ExpressionAtlasiO89093. baseline and differential.
GenevestigatoriO89093.

Interactioni

Subunit structurei

Binds to and activate CCR6.

Structurei

Secondary structure

1
97
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 374Combined sources
Helixi44 – 463Combined sources
Beta strandi47 – 537Combined sources
Beta strandi55 – 584Combined sources
Beta strandi63 – 7614Combined sources
Helixi81 – 9313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HA6NMR-A28-97[»]
ProteinModelPortaliO89093.
SMRiO89093. Positions 28-97.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO89093.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44269.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiO89093.
KOiK14625.
PhylomeDBiO89093.
TreeFamiTF334888.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: O89093-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACGGKRLLF LALAWVLLAH LCSQAEAASN YDCCLSYIQT PLPSRAIVGF
60 70 80 90
TRQMADEACD INAIIFHTKK RKSVCADPKQ NWVKRAVNLL SLRVKKM
Length:97
Mass (Da):10,826
Last modified:November 1, 1998 - v1
Checksum:i6E0C73C8AC80AB0A
GO
Isoform Short (identifier: O89093-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     28-28: Missing.

Show »
Length:96
Mass (Da):10,755
Checksum:iA5236C18BC0E09DB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei28 – 281Missing in isoform Short. CuratedVSP_001062

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053313 mRNA. Translation: AAC78295.1.
AJ007862 Genomic DNA. Translation: CAA07714.1.
AF099052 mRNA. Translation: AAC78680.1.
AJ222694 mRNA. Translation: CAA10948.1.
AB015136 mRNA. Translation: BAA76955.1.
CCDSiCCDS35632.1. [O89093-1]
RefSeqiNP_001153210.1. NM_001159738.1. [O89093-2]
NP_058656.1. NM_016960.2. [O89093-1]
UniGeneiMm.116739.

Genome annotation databases

EnsembliENSMUST00000113437; ENSMUSP00000109064; ENSMUSG00000026166. [O89093-1]
ENSMUST00000186832; ENSMUSP00000139923; ENSMUSG00000026166. [O89093-2]
GeneIDi20297.
KEGGimmu:20297.
UCSCiuc007bsk.2. mouse. [O89093-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053313 mRNA. Translation: AAC78295.1.
AJ007862 Genomic DNA. Translation: CAA07714.1.
AF099052 mRNA. Translation: AAC78680.1.
AJ222694 mRNA. Translation: CAA10948.1.
AB015136 mRNA. Translation: BAA76955.1.
CCDSiCCDS35632.1. [O89093-1]
RefSeqiNP_001153210.1. NM_001159738.1. [O89093-2]
NP_058656.1. NM_016960.2. [O89093-1]
UniGeneiMm.116739.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HA6NMR-A28-97[»]
ProteinModelPortaliO89093.
SMRiO89093. Positions 28-97.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO89093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113437; ENSMUSP00000109064; ENSMUSG00000026166. [O89093-1]
ENSMUST00000186832; ENSMUSP00000139923; ENSMUSG00000026166. [O89093-2]
GeneIDi20297.
KEGGimmu:20297.
UCSCiuc007bsk.2. mouse. [O89093-1]

Organism-specific databases

CTDi6364.
MGIiMGI:1329031. Ccl20.

Phylogenomic databases

eggNOGiNOG44269.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiO89093.
KOiK14625.
PhylomeDBiO89093.
TreeFamiTF334888.

Enzyme and pathway databases

ReactomeiREACT_230248. Chemokine receptors bind chemokines.
REACT_250376. G alpha (i) signalling events.

Miscellaneous databases

EvolutionaryTraceiO89093.
NextBioi298025.
PROiO89093.
SOURCEiSearch...

Gene expression databases

BgeeiO89093.
CleanExiMM_CCL20.
ExpressionAtlasiO89093. baseline and differential.
GenevestigatoriO89093.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel rat CC chemokine, identified by targeted differential display, is upregulated in brain inflammation."
    Utans-Schneitz U., Lorez H., Klinkert W.E.F., da Silva J., Lesslauer W.
    J. Neuroimmunol. 92:179-190(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  2. Villares R.
    Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
  3. "Cloning and characterization of a murine homolog of macrophage inflammatory protein 3 alpha."
    McColl S.R., Carney T., Neote K.S.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM SHORT).
    Strain: BALB/c.
  4. "Molecular cloning, functional characterization and mRNA expression analysis of the murine chemokine receptor CCR6 and its specific ligand MIP-3alpha."
    Varona R., Zaballos A., Gutierrez J., Martin P., Roncal F., Albar J.P., Ardavin C., Marquez G.
    FEBS Lett. 440:188-194(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), CHARACTERIZATION.
    Tissue: Thymus.
  5. "Selective expression of liver and activation-regulated chemokine (LARC) in intestinal epithelium in mice and humans."
    Tanaka Y., Imai T., Baba M., Ishikawa I., Uehira M., Nomiyama H., Yoshie O.
    Eur. J. Immunol. 29:633-642(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, PROTEIN SEQUENCE OF N-TERMINUS.
    Tissue: Liver.
  6. "NMR solution structure of murine CCL20/MIP-3alpha, a chemokine that specifically chemoattracts immature dendritic cells and lymphocytes through its highly specific interaction with the beta-chemokine receptor CCR6."
    Perez-Canadillas J.M., Zaballos A., Gutierrez J., Varona R., Roncal F., Albar J.P., Marquez G., Bruix M.
    J. Biol. Chem. 276:28372-28379(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 28-97, DISULFIDE BONDS.

Entry informationi

Entry nameiCCL20_MOUSE
AccessioniPrimary (citable) accession number: O89093
Secondary accession number(s): Q9Z1X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.