Reviewed, UniProtKB/Swiss-Prot O89084 (PDE4A_MOUSE)

Names and origin

Protein names

Recommended name:
    cAMP-specific 3',5'-cyclic phosphodiesterase 4A
    EC=3.1.4.17

Gene names

Name:

Pde4a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	844 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Enzyme regulation	Inhibited by rolipram and diazepam. Ref.2
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Subunit structure	Interacts with LYN and ARRB2 By similarity.
Subcellular location	Cytoplasm › perinuclear region By similarity.
Post-translational modification	Proteolytically cleaved by caspase-3 By similarity.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: O89084-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O89084-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     235-256: WCLEQLETMQTYRSVSEMASHK → MPLVDFFCETCSKPWLVGWWDQ

Isoform 3 (identifier: O89084-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: MEPPAAPSER...TGTGGGSSRR → MRSSAAPRAR...LRHPPGRCVS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 844	844	cAMP-specific 3',5'-cyclic phosphodiesterase 4A		PRO_0000198807
Regions
	Region	317 – 710	394	Catalytic By similarity
Sites
	Site	69 – 70	2	Cleavage; by caspase-3 By similarity
Amino acid modifications
	Modified residue	672	1	Phosphoserine By similarity
	Modified residue	674	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 234	234	Missing in isoform 2.		VSP_004563
	Alternative sequence	1 – 102	102	MEPPA…GSSRR → MRSSAAPRARPRPPALALPL GPESLTHFSFSEEDTLRHPP GRCVS in isoform 3.		VSP_004562
	Alternative sequence	235 – 256	22	WCLEQ…MASHK → MPLVDFFCETCSKPWLVGWW DQ in isoform 2.		VSP_004564
Experimental info
	Sequence conflict	516	1	R → K in AAF19201. Ref.2
	Sequence conflict	685	1	S → G in AAH27224. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 2. Checksum: 632396C9F70E0F61	FASTA	844	93,558
	10 20 30 40 50 60 MEPPAAPSER SLSLSLPGPR EGQATLKPPP QHLWRQPRTP IRIQQRGYSD SAERSEPERS 70 80 90 100 110 120 PHRPIERADA VDTGDRPGLR TTRMSWPSSF HGTGTGGGSS RRLEAENGPT PSPGRSPLDS 130 140 150 160 170 180 QASPGLMLHA GAATSQRRES FLYRSDSDYD MSPKTMSRNS SVASEAHGED LIVTPFAQVL 190 200 210 220 230 240 ASLRNVRSNF SLLTNVPIPS NKRSPLGGPP SVCKATLSEE TCQQLARETL EELDWCLEQL 250 260 270 280 290 300 ETMQTYRSVS EMASHKFKRM LNRELTHLSE MSRSGNQVSE YISNTFLDKQ HEVEIPSPTP 310 320 330 340 350 360 RQRPFQQPPP AAVQQAQPMS QITGLKKLVH TGSLNINVPR FGVKTDQEDL LAQELENLSK 370 380 390 400 410 420 WGLNIFCVSE YAGGRSLSCI MYTIFQERDL LKKFHIPVDT MMTYMLTLED HYHADVAYHN 430 440 450 460 470 480 SLHAADVLQS THVLLATPAL DAVFTDLEIL AALFAAAIHD VDHPGVSNQF LINTNSELAL 490 500 510 520 530 540 MYNDESVLEN HHLAVGFKLL QEENCDIFQN LSKRQRQSLR KMVIDMVLAT DMSKHMTLLA 550 560 570 580 590 600 DLKTMVETKK VTSSGVLLLD NYSDRIQVLR NMVHCADLSN PTKPLELYRQ WTDRIMAEFF 610 620 630 640 650 660 QQGDRERERG MEISPMCDKH TASVEKSQVG FIDYIVHPLW ETWADLVHPD AQDILDTLED 670 680 690 700 710 720 NRDWYHSAIR QSPSPTLEEE PGVLSDPALP DKFQFELTLE EEDEEDSLEV PGLPCTEETL 730 740 750 760 770 780 LAPHDTRAQA MEQSKVKGQS PAVVEVAESL KQETASAHGA PEESAEAVGH SFSLETSILP 790 800 810 820 830 840 DLRTLSPSEE AQGLLGLPSM AAEVEAPRDH LAAMRACSAC SGTSGDNSAV ISAPGRWGSG GDPA
	Isoform 2. Checksum: 280D59FE2DFD67A5	FASTA	610	68,190
	10 20 30 40 50 60 MPLVDFFCET CSKPWLVGWW DQFKRMLNRE LTHLSEMSRS GNQVSEYISN TFLDKQHEVE 70 80 90 100 110 120 IPSPTPRQRP FQQPPPAAVQ QAQPMSQITG LKKLVHTGSL NINVPRFGVK TDQEDLLAQE 130 140 150 160 170 180 LENLSKWGLN IFCVSEYAGG RSLSCIMYTI FQERDLLKKF HIPVDTMMTY MLTLEDHYHA 190 200 210 220 230 240 DVAYHNSLHA ADVLQSTHVL LATPALDAVF TDLEILAALF AAAIHDVDHP GVSNQFLINT 250 260 270 280 290 300 NSELALMYND ESVLENHHLA VGFKLLQEEN CDIFQNLSKR QRQSLRKMVI DMVLATDMSK 310 320 330 340 350 360 HMTLLADLKT MVETKKVTSS GVLLLDNYSD RIQVLRNMVH CADLSNPTKP LELYRQWTDR 370 380 390 400 410 420 IMAEFFQQGD RERERGMEIS PMCDKHTASV EKSQVGFIDY IVHPLWETWA DLVHPDAQDI 430 440 450 460 470 480 LDTLEDNRDW YHSAIRQSPS PTLEEEPGVL SDPALPDKFQ FELTLEEEDE EDSLEVPGLP 490 500 510 520 530 540 CTEETLLAPH DTRAQAMEQS KVKGQSPAVV EVAESLKQET ASAHGAPEES AEAVGHSFSL 550 560 570 580 590 600 ETSILPDLRT LSPSEEAQGL LGLPSMAAEV EAPRDHLAAM RACSACSGTS GDNSAVISAP 610 GRWGSGGDPA
	Isoform 3. Checksum: 703D813F13AB5E03	FASTA	787	87,193
	10 20 30 40 50 60 MRSSAAPRAR PRPPALALPL GPESLTHFSF SEEDTLRHPP GRCVSLEAEN GPTPSPGRSP 70 80 90 100 110 120 LDSQASPGLM LHAGAATSQR RESFLYRSDS DYDMSPKTMS RNSSVASEAH GEDLIVTPFA 130 140 150 160 170 180 QVLASLRNVR SNFSLLTNVP IPSNKRSPLG GPPSVCKATL SEETCQQLAR ETLEELDWCL 190 200 210 220 230 240 EQLETMQTYR SVSEMASHKF KRMLNRELTH LSEMSRSGNQ VSEYISNTFL DKQHEVEIPS 250 260 270 280 290 300 PTPRQRPFQQ PPPAAVQQAQ PMSQITGLKK LVHTGSLNIN VPRFGVKTDQ EDLLAQELEN 310 320 330 340 350 360 LSKWGLNIFC VSEYAGGRSL SCIMYTIFQE RDLLKKFHIP VDTMMTYMLT LEDHYHADVA 370 380 390 400 410 420 YHNSLHAADV LQSTHVLLAT PALDAVFTDL EILAALFAAA IHDVDHPGVS NQFLINTNSE 430 440 450 460 470 480 LALMYNDESV LENHHLAVGF KLLQEENCDI FQNLSKRQRQ SLRKMVIDMV LATDMSKHMT 490 500 510 520 530 540 LLADLKTMVE TKKVTSSGVL LLDNYSDRIQ VLRNMVHCAD LSNPTKPLEL YRQWTDRIMA 550 560 570 580 590 600 EFFQQGDRER ERGMEISPMC DKHTASVEKS QVGFIDYIVH PLWETWADLV HPDAQDILDT 610 620 630 640 650 660 LEDNRDWYHS AIRQSPSPTL EEEPGVLSDP ALPDKFQFEL TLEEEDEEDS LEVPGLPCTE 670 680 690 700 710 720 ETLLAPHDTR AQAMEQSKVK GQSPAVVEVA ESLKQETASA HGAPEESAEA VGHSFSLETS 730 740 750 760 770 780 ILPDLRTLSP SEEAQGLLGL PSMAAEVEAP RDHLAAMRAC SACSGTSGDN SAVISAPGRW GSGGDPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Physical mapping and promoter structure of the murine cAMP-specific phosphodiesterase pde4a gene." Olsen A.E., Bolger G.B. Mamm. Genome 11:41-45(2000) [PubMed: 10602991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Tissue: Embryonic stem cell.
[2]	"Diazepam and rolipram differentially inhibit cyclic AMP-specific phosphodiesterases PDE4A1 and PDE4B3 in the mouse." Cherry J.A., Thompson B.E., Pho V. Biochim. Biophys. Acta 1518:27-35(2001) [PubMed: 11267656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ENZYME REGULATION. Strain: Swiss Webster. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: FVB/N. Tissue: Salivary gland.
[4]	"Identification and characterization of the human homologue of the short PDE4A cAMP-specific phosphodiesterase 4A variant RD1 (PDE4A1) by analysis of the human HSPDE4A gene locus located at chromosome 19p13.2." Sullivan M., Rena G., Begg F., Gordon L., Olsen A.S., Houslay M.D. Biochem. J. 333:693-703(1998) [PubMed: 9677330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-355 (ISOFORM 2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF142646 , AF142643, AF142644, AF142645 Genomic DNA. Translation: AAF14519.1. AF142646, AF142644, AF142645 Genomic DNA. Translation: AAF14520.1. AF208021 mRNA. Translation: AAF19201.2. AJ297396 mRNA. Translation: CAB96769.1. BC027224 mRNA. Translation: AAH27224.2. Different initiation. U97586 mRNA. Translation: AAC25681.1.
IPI	IPI00130881. IPI00228051. IPI00316902.
RefSeq	NP_062772.3. NP_899668.1.
UniGene	Mm.191749
3D structure databases
HSSP	HSSP built from PDB template 1F0J based on UniProtKB Q07343.
SMR	O89084. Positions 338-671.
ModBase	Search...
Protein-protein interaction databases
STRING	O89084.
PTM databases
PhosphoSite	O89084.
Proteomic databases
PRIDE	O89084.
Genome annotation databases
Ensembl	ENSMUST00000003395; ENSMUSP00000003395; ENSMUSG00000032177; Mus musculus. [Genome view] ENSMUST00000039413; ENSMUSP00000037025; ENSMUSG00000032177; Mus musculus. [Genome view] ENSMUST00000115458; ENSMUSP00000111118; ENSMUSG00000032177; Mus musculus. [Genome view]
GeneID	18577.
KEGG	mmu:18577.
UCSC	uc009oki.1. mouse. uc009okj.1. mouse. uc009okl.1. mouse.
Organism-specific databases
CTD	18577.
MGI	MGI:99558. Pde4a.
Phylogenomic databases
HOGENOM	O89084.
HOVERGEN	O89084.
OMA	HIPVDTM.
Enzyme and pathway databases
BRENDA	3.1.4.17. 244.
Gene expression databases
ArrayExpress	O89084.
Bgee	O89084.
CleanEx	MM_PDE4A.
Genevestigator	O89084.
GermOnline	ENSMUSG00000032177. Mus musculus.
Family and domain databases
InterPro	IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	294438.
SOURCE	Search...

Entry information

Entry name

PDE4A_MOUSE

Accession

Primary (citable) accession number: O89084
Secondary accession number(s): Q8R078 , Q9JHQ4, Q9QX48, Q9QX49, Q9QXI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)