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Reviewed, UniProtKB/Swiss-Prot O89084 (PDE4A_MOUSE)

Last modified November 3, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    cAMP-specific 3',5'-cyclic phosphodiesterase 4A
    EC=3.1.4.17
Gene names
Name: Pde4a
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length844 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Enzyme regulation

Inhibited by rolipram and diazepam. Ref.2

Pathway

Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Subunit structure

Interacts with LYN and ARRB2 By similarity.

Subcellular location

Cytoplasmperinuclear region By similarity.

Post-translational modification

Proteolytically cleaved by caspase-3 By similarity.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandcAMP
   Molecular functionHydrolase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processsignal transduction

Inferred from electronic annotation. Source: InterPro

   Molecular function3',5'-cyclic-nucleotide phosphodiesterase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: O89084-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O89084-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     235-256: WCLEQLETMQTYRSVSEMASHK → MPLVDFFCETCSKPWLVGWWDQ
Isoform 3 (identifier: O89084-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: MEPPAAPSER...TGTGGGSSRR → MRSSAAPRAR...LRHPPGRCVS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 844844cAMP-specific 3',5'-cyclic phosphodiesterase 4A
PRO_0000198807

Regions

Region317 – 710394Catalytic By similarity

Sites

Site69 – 702Cleavage; by caspase-3 By similarity

Amino acid modifications

Modified residue6721Phosphoserine By similarity
Modified residue6741Phosphoserine By similarity

Natural variations

Alternative sequence1 – 234234Missing in isoform 2.
VSP_004563
Alternative sequence1 – 102102MEPPA…GSSRR → MRSSAAPRARPRPPALALPL GPESLTHFSFSEEDTLRHPP GRCVS in isoform 3.
VSP_004562
Alternative sequence235 – 25622WCLEQ…MASHK → MPLVDFFCETCSKPWLVGWW DQ in isoform 2.
VSP_004564

Experimental info

Sequence conflict5161R → K in AAF19201. Ref.2
Sequence conflict6851S → G in AAH27224. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 2.
Checksum: 632396C9F70E0F61

FASTA84493,558
        10         20         30         40         50         60 
MEPPAAPSER SLSLSLPGPR EGQATLKPPP QHLWRQPRTP IRIQQRGYSD SAERSEPERS 

        70         80         90        100        110        120 
PHRPIERADA VDTGDRPGLR TTRMSWPSSF HGTGTGGGSS RRLEAENGPT PSPGRSPLDS 

       130        140        150        160        170        180 
QASPGLMLHA GAATSQRRES FLYRSDSDYD MSPKTMSRNS SVASEAHGED LIVTPFAQVL 

       190        200        210        220        230        240 
ASLRNVRSNF SLLTNVPIPS NKRSPLGGPP SVCKATLSEE TCQQLARETL EELDWCLEQL 

       250        260        270        280        290        300 
ETMQTYRSVS EMASHKFKRM LNRELTHLSE MSRSGNQVSE YISNTFLDKQ HEVEIPSPTP 

       310        320        330        340        350        360 
RQRPFQQPPP AAVQQAQPMS QITGLKKLVH TGSLNINVPR FGVKTDQEDL LAQELENLSK 

       370        380        390        400        410        420 
WGLNIFCVSE YAGGRSLSCI MYTIFQERDL LKKFHIPVDT MMTYMLTLED HYHADVAYHN 

       430        440        450        460        470        480 
SLHAADVLQS THVLLATPAL DAVFTDLEIL AALFAAAIHD VDHPGVSNQF LINTNSELAL 

       490        500        510        520        530        540 
MYNDESVLEN HHLAVGFKLL QEENCDIFQN LSKRQRQSLR KMVIDMVLAT DMSKHMTLLA 

       550        560        570        580        590        600 
DLKTMVETKK VTSSGVLLLD NYSDRIQVLR NMVHCADLSN PTKPLELYRQ WTDRIMAEFF 

       610        620        630        640        650        660 
QQGDRERERG MEISPMCDKH TASVEKSQVG FIDYIVHPLW ETWADLVHPD AQDILDTLED 

       670        680        690        700        710        720 
NRDWYHSAIR QSPSPTLEEE PGVLSDPALP DKFQFELTLE EEDEEDSLEV PGLPCTEETL 

       730        740        750        760        770        780 
LAPHDTRAQA MEQSKVKGQS PAVVEVAESL KQETASAHGA PEESAEAVGH SFSLETSILP 

       790        800        810        820        830        840 
DLRTLSPSEE AQGLLGLPSM AAEVEAPRDH LAAMRACSAC SGTSGDNSAV ISAPGRWGSG 


GDPA 

« Hide

Isoform 2.

Checksum: 280D59FE2DFD67A5
Show »

FASTA61068,190
Isoform 3.

Checksum: 703D813F13AB5E03
Show »

FASTA78787,193

References

« Hide 'large scale' references
[1]"Physical mapping and promoter structure of the murine cAMP-specific phosphodiesterase pde4a gene."
Olsen A.E., Bolger G.B.
Mamm. Genome 11:41-45(2000) [PubMed: 10602991] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Tissue: Embryonic stem cell.
[2]"Diazepam and rolipram differentially inhibit cyclic AMP-specific phosphodiesterases PDE4A1 and PDE4B3 in the mouse."
Cherry J.A., Thompson B.E., Pho V.
Biochim. Biophys. Acta 1518:27-35(2001) [PubMed: 11267656] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ENZYME REGULATION.
Strain: Swiss Webster.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: FVB/N.
Tissue: Salivary gland.
[4]"Identification and characterization of the human homologue of the short PDE4A cAMP-specific phosphodiesterase 4A variant RD1 (PDE4A1) by analysis of the human HSPDE4A gene locus located at chromosome 19p13.2."
Sullivan M., Rena G., Begg F., Gordon L., Olsen A.S., Houslay M.D.
Biochem. J. 333:693-703(1998) [PubMed: 9677330] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-355 (ISOFORM 2).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF142646 expand/collapse EMBL AC list , AF142643, AF142644, AF142645 Genomic DNA. Translation: AAF14519.1.
AF142646, AF142644, AF142645 Genomic DNA. Translation: AAF14520.1.
AF208021 mRNA. Translation: AAF19201.2.
AJ297396 mRNA. Translation: CAB96769.1.
BC027224 mRNA. Translation: AAH27224.2. Different initiation.
U97586 mRNA. Translation: AAC25681.1.
IPIIPI00130881.
IPI00228051.
IPI00316902.
RefSeqNP_062772.3.
NP_899668.1.
UniGeneMm.191749

3D structure databases

HSSPHSSP built from PDB template 1F0J based on UniProtKB Q07343.
SMRO89084. Positions 338-671.
ModBaseSearch...

Protein-protein interaction databases

STRINGO89084.

PTM databases

PhosphoSiteO89084.

Proteomic databases

PRIDEO89084.

Genome annotation databases

EnsemblENSMUST00000003395; ENSMUSP00000003395; ENSMUSG00000032177; Mus musculus. [Genome view]
ENSMUST00000039413; ENSMUSP00000037025; ENSMUSG00000032177; Mus musculus. [Genome view]
ENSMUST00000115458; ENSMUSP00000111118; ENSMUSG00000032177; Mus musculus. [Genome view]
GeneID18577.
KEGGmmu:18577.
UCSCuc009oki.1. mouse.
uc009okj.1. mouse.
uc009okl.1. mouse.

Organism-specific databases

CTD18577.
MGIMGI:99558. Pde4a.

Phylogenomic databases

HOGENOMO89084.
HOVERGENO89084.
OMAHIPVDTM.

Enzyme and pathway databases

BRENDA3.1.4.17. 244.

Gene expression databases

ArrayExpressO89084.
BgeeO89084.
CleanExMM_PDE4A.
GenevestigatorO89084.
GermOnlineENSMUSG00000032177. Mus musculus.

Family and domain databases

InterProIPR003607. Met-dep_phosphohydro_HD.
IPR002073. PDEase.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio294438.
SOURCESearch...

Entry information

Entry namePDE4A_MOUSE
AccessionPrimary (citable) accession number: O89084
Secondary accession number(s): Q8R078 expand/collapse secondary AC list , Q9JHQ4, Q9QX48, Q9QX49, Q9QXI8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: March 1, 2002
Last modified: November 3, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents