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Protein

Coatomer subunit epsilon

Gene

Cope

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit epsilon
Alternative name(s):
Epsilon-coat protein
Short name:
Epsilon-COP
Gene namesi
Name:Cope
Synonyms:Cope1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1891702. Cope.

Subcellular locationi

GO - Cellular componenti

  • COPI-coated vesicle Source: MGI
  • COPI-coated vesicle membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 308307Coatomer subunit epsilonPRO_0000193852Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by PKA.By similarity
Polyubiquitinated by RCHY1 in the presence of androgen, leading to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO89079.
MaxQBiO89079.
PaxDbiO89079.
PRIDEiO89079.

2D gel databases

REPRODUCTION-2DPAGEO89079.

PTM databases

iPTMnetiO89079.
PhosphoSiteiO89079.
SwissPalmiO89079.

Expressioni

Gene expression databases

BgeeiO89079.
CleanExiMM_COPE.
ExpressionAtlasiO89079. baseline and differential.
GenevisibleiO89079. MM.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.

Protein-protein interaction databases

IntActiO89079. 1 interaction.
MINTiMINT-1865171.
STRINGi10090.ENSMUSP00000071078.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5A1Velectron microscopy21.00Q/Z1-308[»]
5A1Yelectron microscopy21.00X/Z1-308[»]
ProteinModelPortaliO89079.
SMRiO89079. Positions 17-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the COPE family.Curated

Phylogenomic databases

eggNOGiKOG3081. Eukaryota.
ENOG410XS5P. LUCA.
GeneTreeiENSGT00390000003478.
HOGENOMiHOG000205825.
HOVERGENiHBG002201.
InParanoidiO89079.
KOiK17268.
OMAiDAFHIYQ.
OrthoDBiEOG7QK0CC.
PhylomeDBiO89079.
TreeFamiTF313390.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR006822. Coatomer_esu.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR10805. PTHR10805. 1 hit.
PIRSFiPIRSF016478. Coatomer_esu. 1 hit.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O89079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPVPGAVS GGSGEVDELF DVKNAFYIGS YQQCINEAQR VKLSSPEREV
60 70 80 90 100
ERDVFLYRAY LAQRKYGVVL DEIKPSSAPE LQAVRMFAEY LASENQRDSI
110 120 130 140 150
VLELDREMSR SVDVTNTTFL LMAASIYFHD QNPDAALRTL HQGDGLECMA
160 170 180 190 200
MTIQILLKLD RLDLARKELK KMQDQDEDAT LTQLATAWVN LAVGGEKLQE
210 220 230 240 250
AYYIFQELAD KCSPTLLLLN GQAACHSAQG RWETAEGVLQ EALDKDSGHP
260 270 280 290 300
ETLINLIVLS QHLGKPPEVT NRYLSQLKDA HRAHPFIKEY QAKENDFDRL

AMQYAPSA
Length:308
Mass (Da):34,567
Last modified:January 23, 2007 - v3
Checksum:i81331BDDB1F99270
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti291 – 2911Q → R in AAC36533 (PubMed:9798653).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039837 mRNA. Translation: BAA92384.1.
BC009170 mRNA. Translation: AAH09170.1.
BC083336 mRNA. Translation: AAH83336.1.
U89427 mRNA. Translation: AAC36533.1.
CCDSiCCDS22365.1.
RefSeqiNP_067513.1. NM_021538.1.
UniGeneiMm.28668.

Genome annotation databases

EnsembliENSMUST00000066469; ENSMUSP00000071078; ENSMUSG00000055681.
GeneIDi59042.
KEGGimmu:59042.
UCSCiuc009lzx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039837 mRNA. Translation: BAA92384.1.
BC009170 mRNA. Translation: AAH09170.1.
BC083336 mRNA. Translation: AAH83336.1.
U89427 mRNA. Translation: AAC36533.1.
CCDSiCCDS22365.1.
RefSeqiNP_067513.1. NM_021538.1.
UniGeneiMm.28668.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5A1Velectron microscopy21.00Q/Z1-308[»]
5A1Yelectron microscopy21.00X/Z1-308[»]
ProteinModelPortaliO89079.
SMRiO89079. Positions 17-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO89079. 1 interaction.
MINTiMINT-1865171.
STRINGi10090.ENSMUSP00000071078.

PTM databases

iPTMnetiO89079.
PhosphoSiteiO89079.
SwissPalmiO89079.

2D gel databases

REPRODUCTION-2DPAGEO89079.

Proteomic databases

EPDiO89079.
MaxQBiO89079.
PaxDbiO89079.
PRIDEiO89079.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066469; ENSMUSP00000071078; ENSMUSG00000055681.
GeneIDi59042.
KEGGimmu:59042.
UCSCiuc009lzx.1. mouse.

Organism-specific databases

CTDi11316.
MGIiMGI:1891702. Cope.

Phylogenomic databases

eggNOGiKOG3081. Eukaryota.
ENOG410XS5P. LUCA.
GeneTreeiENSGT00390000003478.
HOGENOMiHOG000205825.
HOVERGENiHBG002201.
InParanoidiO89079.
KOiK17268.
OMAiDAFHIYQ.
OrthoDBiEOG7QK0CC.
PhylomeDBiO89079.
TreeFamiTF313390.

Enzyme and pathway databases

ReactomeiR-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiO89079.
SOURCEiSearch...

Gene expression databases

BgeeiO89079.
CleanExiMM_COPE.
ExpressionAtlasiO89079. baseline and differential.
GenevisibleiO89079. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR006822. Coatomer_esu.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR10805. PTHR10805. 1 hit.
PIRSFiPIRSF016478. Coatomer_esu. 1 hit.
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Cope1 gene for nonclathrin coat protein epsilon-COP."
    Hahn Y., Chung J.H.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Mammary tumor.
  3. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
    Chu C.C., Paul W.E.
    Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 155-298.
    Strain: BALB/cJ.
    Tissue: Spleen.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiCOPE_MOUSE
AccessioniPrimary (citable) accession number: O89079
Secondary accession number(s): Q9JM65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.