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Protein

Muskelin

Gene

Mkln1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1.1 Publication

GO - Biological processi

  • actin cytoskeleton reorganization Source: UniProtKB
  • cell-matrix adhesion Source: UniProtKB
  • regulation of cell shape Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Muskelin
Gene namesi
Name:Mkln1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1351638. Mkln1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell projectionruffle 1 Publication
  • Cytoplasmcell cortex 1 Publication

  • Note: Colocalizes with actin fibers in the cell cortex.1 Publication

GO - Cellular componenti

  • cell cortex Source: UniProtKB
  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001191392 – 735MuskelinAdd BLAST734

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO89050.
MaxQBiO89050.
PaxDbiO89050.
PeptideAtlasiO89050.
PRIDEiO89050.

PTM databases

iPTMnetiO89050.
PhosphoSitePlusiO89050.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025609.
ExpressionAtlasiO89050. baseline and differential.
GenevisibleiO89050. MM.

Interactioni

Subunit structurei

Interacts with the C-terminal tail of the prostaglandin EP3 receptor alpha isoform (By similarity). Interacts with RANBP9. Part of a complex consisting of RANBP9, MKLN1 and GID8.By similarity

Protein-protein interaction databases

BioGridi205222. 8 interactors.
DIPiDIP-61380N.
IntActiO89050. 6 interactors.
STRINGi10090.ENSMUSP00000026699.

Structurei

Secondary structure

1735
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 24Combined sources7
Helixi32 – 36Combined sources5
Beta strandi45 – 48Combined sources4
Beta strandi52 – 54Combined sources3
Beta strandi58 – 73Combined sources16
Beta strandi83 – 94Combined sources12
Beta strandi97 – 104Combined sources8
Beta strandi108 – 110Combined sources3
Beta strandi113 – 116Combined sources4
Beta strandi128 – 141Combined sources14
Beta strandi150 – 155Combined sources6
Helixi158 – 161Combined sources4
Helixi162 – 164Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PQQX-ray1.55A12-167[»]
ProteinModelPortaliO89050.
SMRiO89050.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 204LisHPROSITE-ProRule annotationAdd BLAST33
Domaini206 – 258CTLHPROSITE-ProRule annotationAdd BLAST53
Repeati284 – 330Kelch 1Add BLAST47
Repeati339 – 391Kelch 2Add BLAST53
Repeati400 – 458Kelch 3Add BLAST59
Repeati469 – 515Kelch 4Add BLAST47
Repeati526 – 578Kelch 5Add BLAST53
Repeati597 – 651Kelch 6Add BLAST55

Sequence similaritiesi

Contains 1 CTLH domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated
Contains 1 LisH domain.PROSITE-ProRule annotation

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG2437. Eukaryota.
ENOG410YJKC. LUCA.
GeneTreeiENSGT00390000001702.
HOGENOMiHOG000047912.
HOVERGENiHBG052481.
InParanoidiO89050.
OMAiNFHKLAS.
OrthoDBiEOG091G07E6.
PhylomeDBiO89050.
TreeFamiTF323659.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR006595. CTLH_C.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR008979. Galactose-bd-like.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR006594. LisH.
IPR010565. Muskelin_N.
[Graphical view]
PfamiPF01344. Kelch_1. 1 hit.
PF06588. Muskelin_N. 1 hit.
[Graphical view]
SMARTiSM00667. LisH. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50965. SSF50965. 1 hit.
PROSITEiPS50897. CTLH. 1 hit.
PS50896. LISH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O89050-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGGAVAVA PECRLLPYAL HKWSSFSSTY LPENILVDKP NDQSSRWSSE
60 70 80 90 100
SNYPPQYLIL KLERPAIVQN ITFGKYEKTH VCNLKKFKVF GGMNEENMTE
110 120 130 140 150
LLSSGLKNDY NKETFTLKHK IDEQMFPCRF IKIVPLLSWG PSFNFSIWYV
160 170 180 190 200
ELSGIDDPDI VQPCLNWYSK YREQEAIRLC LKHFRQHNYT EAFESLQKKT
210 220 230 240 250
KIALEHPMLT DMHDKLVLKG DFDACEELIE KAVNDGLFNQ YISQQEYKPR
260 270 280 290 300
WSQIIPKSTK GDGEDNRPGM RGGHQMVIDV QTETVYLFGG WDGTQDLADF
310 320 330 340 350
WAYSVKENQW TCISRDTEKE NGPSARSCHK MCIDIQRRQI YTLGRYLDSS
360 370 380 390 400
VRNSKSLKSD FYRYDIDTNT WMLLSEDTAA DGGPKLVFDH QMCMDSEKHM
410 420 430 440 450
IYTFGGRILT CNGSVDDSRA SEPQFSGLFA FNCQCQTWKL LREDSCNAGP
460 470 480 490 500
EDIQSRIGHC MLFHSKNRCL YVFGGQRSKT YLNDFFSYDV DSDHVDIISD
510 520 530 540 550
GTKKDSGMVP MTGFTQRATI DPELNEIHVL SGLSKDKEKR EENVRNSFWI
560 570 580 590 600
YDIVRNSWSC VYKNDQATKD NLSKSLQEEE PCPRFAHQLV YDELHKVHYL
610 620 630 640 650
FGGNPGKSCS PKMRLDDFWS LKLCRPSKDY LLRHCKYLIR KHRFEEKAQM
660 670 680 690 700
DPLSALKYLQ NDLYITVDHS DPEETKEFQL LASALFKSGS DFTALGFSDV
710 720 730
DHTYAQRTQL FDTLVNFFPD SMTPPKGNLV DLITL
Length:735
Mass (Da):84,878
Last modified:November 1, 1998 - v1
Checksum:i7B0C8D2CB8C242AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72194 mRNA. Translation: AAC63849.1.
BC013703 mRNA. Translation: AAH13703.1.
CCDSiCCDS19982.1.
RefSeqiNP_038819.1. NM_013791.2.
UniGeneiMm.238038.
Mm.392838.
Mm.440531.

Genome annotation databases

EnsembliENSMUST00000026699; ENSMUSP00000026699; ENSMUSG00000025609.
GeneIDi27418.
KEGGimmu:27418.
UCSCiuc009bgf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72194 mRNA. Translation: AAC63849.1.
BC013703 mRNA. Translation: AAH13703.1.
CCDSiCCDS19982.1.
RefSeqiNP_038819.1. NM_013791.2.
UniGeneiMm.238038.
Mm.392838.
Mm.440531.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PQQX-ray1.55A12-167[»]
ProteinModelPortaliO89050.
SMRiO89050.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205222. 8 interactors.
DIPiDIP-61380N.
IntActiO89050. 6 interactors.
STRINGi10090.ENSMUSP00000026699.

PTM databases

iPTMnetiO89050.
PhosphoSitePlusiO89050.

Proteomic databases

EPDiO89050.
MaxQBiO89050.
PaxDbiO89050.
PeptideAtlasiO89050.
PRIDEiO89050.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026699; ENSMUSP00000026699; ENSMUSG00000025609.
GeneIDi27418.
KEGGimmu:27418.
UCSCiuc009bgf.1. mouse.

Organism-specific databases

CTDi4289.
MGIiMGI:1351638. Mkln1.

Phylogenomic databases

eggNOGiKOG2437. Eukaryota.
ENOG410YJKC. LUCA.
GeneTreeiENSGT00390000001702.
HOGENOMiHOG000047912.
HOVERGENiHBG052481.
InParanoidiO89050.
OMAiNFHKLAS.
OrthoDBiEOG091G07E6.
PhylomeDBiO89050.
TreeFamiTF323659.

Miscellaneous databases

ChiTaRSiMkln1. mouse.
PROiO89050.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025609.
ExpressionAtlasiO89050. baseline and differential.
GenevisibleiO89050. MM.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR006595. CTLH_C.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR008979. Galactose-bd-like.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR006594. LisH.
IPR010565. Muskelin_N.
[Graphical view]
PfamiPF01344. Kelch_1. 1 hit.
PF06588. Muskelin_N. 1 hit.
[Graphical view]
SMARTiSM00667. LisH. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50965. SSF50965. 1 hit.
PROSITEiPS50897. CTLH. 1 hit.
PS50896. LISH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMKLN1_MOUSE
AccessioniPrimary (citable) accession number: O89050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.