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O89047 (KCNH3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily H member 3
Alternative name(s):
Brain-specific eag-like channel 1
Short name=BEC1
Ether-a-go-go-like potassium channel 2
Short name=ELK channel 2
Short name=rElk2
Voltage-gated potassium channel subunit Kv12.2
Gene names
Name:Kcnh3
Synonyms:Elk2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1087 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits an outward current with fast inactivation. Channel properties may be modulated by cAMP and subunit assembly.

Subunit structure

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Highly expressed in adult and embryonic brain, in particular in cerebellum, brain stem, hippocampus, cortex and striatum. Detected at slightly lower levels in heart, spinal cord, olfactory bulb, pituitary and medulla. In the hippocampus expression is strongest in the pyramidal cell body layers of the dentate gyrus. Also found in pituitary. Ref.4

Domain

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similarities

Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv12.2/KCNH3 sub-subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10871087Potassium voltage-gated channel subfamily H member 3
PRO_0000054007

Regions

Topological domain1 – 228228Cytoplasmic Potential
Transmembrane229 – 24921Helical; Name=Segment S1; Potential
Topological domain250 – 25910Extracellular Potential
Transmembrane260 – 28021Helical; Name=Segment S2; Potential
Topological domain281 – 30222Cytoplasmic Potential
Transmembrane303 – 32321Helical; Name=Segment S3; Potential
Topological domain324 – 3318Extracellular Potential
Transmembrane332 – 35221Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain353 – 3619Cytoplasmic Potential
Transmembrane362 – 38221Helical; Name=Segment S5; Potential
Topological domain383 – 45674Extracellular Potential
Intramembrane457 – 47721Pore-forming; Name=Segment H5; Potential
Topological domain478 – 4825Extracellular Potential
Transmembrane483 – 50321Helical; Name=Segment S6; Potential
Topological domain504 – 1087584Cytoplasmic Potential
Domain18 – 9073PAS
Domain93 – 14553PAC
Nucleotide binding585 – 700116cNMP
Motif468 – 4736Selectivity filter By similarity
Compositional bias954 – 1061108Pro-rich

Amino acid modifications

Glycosylation4211N-linked (GlcNAc...) Potential
Glycosylation4281N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O89047 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 16B5AFFC3B0A405B

FASTA1,087117,580
        10         20         30         40         50         60 
MPAMRGLLAP QNTFLDTIAT RFDGTHSNFV LGNAQVAGLF PVVYCSDGFC DLTGFSRAEV 

        70         80         90        100        110        120 
MQRGCACSFL YGPDTSELVR QQIRKALDEH KEFKAELILY RKSGLPFWCL LDVIPIKNEK 

       130        140        150        160        170        180 
GEVALFLVSH KDISETKNRG GPDNWKERGG GRRRYGRAGS KGFNANRRRS RAVLYHLSGH 

       190        200        210        220        230        240 
LQKQPKGKHK LNKGVFGEKP NLPEYKVAAI RKSPFILLHC GALRATWDGF ILLATLYVAV 

       250        260        270        280        290        300 
TVPYSVCVST AREPSAARGP PSVCDLAVEV LFILDIVLNF RTTFVSKSGQ VVFAPKSICL 

       310        320        330        340        350        360 
HYVTTWFLLD VIAALPFDLL HAFKVNVYVG AHLLKTVRLL RLLRLLPRLD RYSQYSAVVL 

       370        380        390        400        410        420 
TLLMAVFALL AHWVACVWFY IGQQEIENSE SELPEIGWLQ ELARRLETPY YLVSRSPDGG 

       430        440        450        460        470        480 
NSSGQSENCS SSGGGSEANG TGLELLGGPS LRSAYITSLY FALSSLTSVG FGNVSANTDT 

       490        500        510        520        530        540 
EKIFSICTML IGALMHAVVF GNVTAIIQRM YARRFLYHSR TRDLRDYIRI HRIPKPLKQR 

       550        560        570        580        590        600 
MLEYFQATWA VNNGIDTTEL LQSLPDELRA DIAMHLHKEV LQLPLFEAAS RGCLRALSLA 

       610        620        630        640        650        660 
LRPAFCTPGE YLIHQGDALQ ALYFVCSGSM EVLKGGTVLA ILGKGDLIGC ELPQREQVVK 

       670        680        690        700        710        720 
ANADVKGLTY CVLQCLQLAG LHESLALYPE FAPRFSRGLR GELSYNLGAG GVSAEVDTSS 

       730        740        750        760        770        780 
LSGDNTLMST LEEKETDGEQ GHTISPAPAD EPSSPLLSPG CTSSSSAAKL LSPRRTAPRP 

       790        800        810        820        830        840 
RLGGRGRPSR AGVLKPEAGP SAHPRTLDGL QLPPMPWNVP PDLSPRVVDG IEDGCGSDQH 

       850        860        870        880        890        900 
KFSFRVGQSG PECSSSPSPG TESGLLTVPL VPSEARNTDT LDKLRQAVTE LSEQVLQMRE 

       910        920        930        940        950        960 
GLQSLRQAVQ LILVPQGEGQ CPRVSGEGPC PATASGLLQP LRVDTGASSY CLQPPAGSVL 

       970        980        990       1000       1010       1020 
SGTWPHPRPG HPPPLMAPWP WGPPASQSSP WPRATALWTS TSDSEPPGSG DLCSEPSTPA 

      1030       1040       1050       1060       1070       1080 
SPPPPEEGAR TGTPAPVSQA EATSTGEPPP GSGGRALPWD PHSLEMVLIG CHGPGSVQWT 


QEEGTGV 

« Hide

References

[1]"Cloning and functional expression of rat ether-a-go-go-like K+ channel genes."
Engeland B., Neu A., Ludwig J., Roeper J., Pongs O.
J. Physiol. (Lond.) 513:647-654(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain cortex.
[2]"New ether-a-go-go K+ channel family members localized in human telencephalon."
Miyake A., Mochizuki S., Yokoi H., Kohda M., Furuichi K.
J. Biol. Chem. 274:25018-25025(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia."
Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E.
J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-273.
Tissue: Brain.
[4]"Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ007627 mRNA. Translation: CAA07586.1.
AB022697 mRNA. Translation: BAA83591.1.
AF073892 mRNA. Translation: AAC61522.1.
PIRT31100.
RefSeqNP_058804.1. NM_017108.1.
UniGeneRn.144567.

3D structure databases

ProteinModelPortalO89047.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000047211.

Proteomic databases

PaxDbO89047.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000045515; ENSRNOP00000047211; ENSRNOG00000032075.
GeneID27150.
KEGGrno:27150.
UCSCRGD:71070. rat.

Organism-specific databases

CTD23416.
RGD71070. Kcnh3.

Phylogenomic databases

eggNOGCOG2202.
GeneTreeENSGT00680000099929.
HOGENOMHOG000230794.
HOVERGENHBG052232.
InParanoidO89047.
KOK04906.
OMAPLMAPWP.
OrthoDBEOG7QG43V.
PhylomeDBO89047.
TreeFamTF313130.

Gene expression databases

GenevestigatorO89047.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003950. K_chnl_volt-dep_ELK.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSPR01463. EAGCHANLFMLY.
PR01465. ELKCHANNEL.
SMARTSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608061.
PROO89047.

Entry information

Entry nameKCNH3_RAT
AccessionPrimary (citable) accession number: O89047
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families